A preparation method of uricase-modified titanium diboride composite paste electrode sensor

Abstract

The invention discloses a preparation method of a uricase-modified titanium diboride composite paste electrode sensor. The preparation method is characterized by comprising the following steps: firstly preparing a nano-TiB2 / carbon nanotube composite paste electrode from nano-TiB2, oxidized carbon nanotubes and 1-propenyl-3-methylimidazolium tetrafluoroborate; carrying out surface modification on the nano-TiB2 / carbon nanotube composite paste electrode by virtue of 3-chloropropyltrimethoxysilane, so as to obtain a 3-chloropropyltrimethoxysilane-modified nano-TiB2 / carbon nanotube composite pasteelectrode; and modifying the 3-chloropropyltrimethoxysilane-modified nano-TiB2 / carbon nanotube composite paste electrode by virtue of uricase stationary liquid prepared from a Tris buffer solution, gelatin and bovine serum albumin, so as to obtain the uricase-modified titanium diboride composite paste electrode sensor, wherein the pH value of the Tris buffer solution is 8.5. The electrode sensor prepared by virtue of the preparation method has higher conductivity than a common carbon paste electrode, has specific recognition ability to uric acid and is high in sensitivity.

Description

technical field

[0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of a uricase-modified titanium diboride composite paste electrode sensor. It belongs to the field of electrochemical analysis. Background technique

[0002] Under normal circumstances, there are about 1,200 mg of uric acid in the body, about 600 mg is newly generated every day, and 600 mg is excreted at the same time, which is in a state of balance. However, if there is too much uric acid in the body to excrete or the mechanism of uric acid excretion degrades, the body will retain too much uric acid. When the concentration of uric acid in the blood is high, the body fluids will become acidic and affect the normal function of human cells. If left alone for a long time, it will cause gout. In addition, excessive fatigue or lack of rest can also lead to relatively slow metabolism and lead to gout. Too high or too low uric aci...