Silver vanadate, preparation method thereof and application of silver vanadate in detection of uric acid as peroxidase-like enzyme

Abstract

The invention discloses silver vanadate, a preparation method thereof and application of silver vanadate in detection of uric acid as a peroxidase-like enzyme. The molecular formula of silver vanadateis Ag2V4O11. The preparation method of silver vanadate is characterized by comprising the following steps: (1) respectively dissolving 0.002mol of NH4VO3 and 0.001mol of AgNO3 in 50mL and 20mL of deionized water under the condition of magnetic stirring; (2) dropwise adding the AgNO3 solution obtained in the step (1) into the NH4VO3 solution under the condition of magnetic stirring; (3) adjustingthe pH value of the solution obtained in the step (2) to 3 by using a 1mol / L HNO3 solution; (4) continuing to stir the reaction solution at room temperature for 1 hour and cure the reaction solution for 4 hours; (5) transferring the reaction solution into a 80mL hydrothermal kettle, and carrying out hydrothermal treatment at 150 DEG C for 24 hours; and (6) cooling the reaction solution to room temperature, carrying out vacuum filtration to obtain a product, cleaning the product with deionized water for many times, and drying the product in a drying oven at 70 DEG C for 12 hours to obtain the silver vanadate with the molecular formula of Ag2V4O11. The preparation method has the advantages and beneficial effects that the synthesized Ag2V4O11 nanobelt is proved to have the activity of a peroxidase-like enzyme for the first time, and the cost is reduced compared with the cost of detection by simply adopting noble metal as mimic enzyme.

Description

Technical field

[0001] The invention relates to the technical field of biological detection and the field of composite oxides, and specifically refers to a preparation method of silver vanadate and its application as a peroxidase-like detection of uric acid. Background technique

[0002] Horseradish peroxidase (HRP) is a type of oxidoreductase produced by microorganisms or plants. It uses hydrogen peroxide as an electron acceptor to catalyze the oxidation of substrates. HRP is one of the most commonly used tool enzymes in immunoenzyme labeling technology, and is often used to label antibodies. HRP is a kind of protein with a high degree of biocatalysis. Therefore, its structure is susceptible to environmental changes, leading to enzyme inactivation. In addition, HRP has the disadvantages of being difficult to purify, high cost, difficult to store, and not conducive to antibody and antigen binding. These shortcomings also limit its wide range of applications.

[0003] In the prio...

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