Alkaline urate oxidase and applications thereof to detection kit and reduction of uric acid in foods

A uric acid oxidase and detection kit technology, applied in the biological field, can solve the problems of narrow pH applicable range, narrow temperature applicable range, poor storage stability, etc., to reduce enzyme usage, improve work efficiency, and high substrate affinity. sexual effect
CN109554376AActive Publication Date: 2019-04-02GUANGXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI UNIV
Publication Date
2019-04-02

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Abstract

The invention relates to an alkaline urate oxidase and applications thereof to a detection kit and the reduction of uric acid in foods. The nucleotide sequence is as shown in the sequence SEQ ID No.1,and the amino acid sequence is as shown in the sequence SEQ ID No.2. A yeast urate oxidase is recombined into the highly-activity alkaline urate oxidase which is capable of degrading, measuring and detecting samples in an alkaline environment, has a wider alkaline measurement range and higher catalytic efficiency than previously-reported enzymes, and is advantageous for improving production efficiency, reducing costs and facilitating industrial application better. According to the kit which utilizes the alkaline urate oxidase and detects uric acid content by a colorimetric method, the kit hasstrong specificity, high sensitivity and simple method. The alkaline urate oxidase is used to develop low-purine foods, and has high application value in low-purine foods.
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Description

technical field

[0001] The invention relates to the fields of biotechnology and food, in particular to an alkaline urate oxidase and its application in a detection kit and reducing uric acid in food. Background technique

[0002] Urate oxidase, Urate oxidase, referred to as UOX; EC.1.7.3.3, is an enzyme in the purine metabolic pathway in organisms, which catalyzes the decomposition of uric acid, water and oxygen into allantoin and hydrogen peroxide. UOX has important commercial application value. UOX has strong specificity and high catalytic efficiency. It is used for clinical detection of serum uric acid, uric acid sensor detection based on immobilized urate oxidase, uric acid lowering treatment for gout patients, and thrombolysis for cerebral infarction. Treatment, etc. (Kai Lei, Yue Ke, Ma Xiaohang. Research and Application of Urate Oxidase. Science and Technology Bulletin, 2008(02):183-187).

[0003] Uric acid is the final product of purine metabolism in the human body ...

Claims

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