Preparation method of nanogold cluster and sensitization detection method of nanogold cluster in small biological molecules
A gold nano-cluster and gold nano-cluster technology, applied in the field of nano-gold clusters, can solve the problems of expensive enzyme, inconvenient storage, complicated electrochemical method operation, etc., and achieve the effect of sensitizing detection of uric acid content
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Embodiment 1
[0020] Preparation of gold nanoclusters
[0021] Prepare 100mmol / L HAuCl4 and 2mol / L NaOH solution. Add 200mg of BSA to 10mL of ultrapure water, stir in a water bath at 40°C until dissolved, quickly add 10mL of 100mmol / L HAuCl4 aqueous solution to the BSA solution under vigorous stirring, mix for 10min, then add 0.5mL of 2mol / L NaOH solution , stirred gently at 37°C for 40h.
[0022] Nanocluster Separation and Purification
[0023] The prepared nano-gold clusters were stored in the dark for 48 hours, and then poured into a 20,000MWCO dialysis bag for dialysis for 48 hours (the water was changed every 8 hours). After dialysis, the mixed solution was filtered through a 0.22 μm microporous membrane with a syringe to remove excess impurities with large particle sizes. The finally obtained solution was stored at 4°C in the dark.
[0024] Experimental Materials
[0025] sample:
[0026] The BSA-AuNCs produced in Example 1 above. Blood samples were taken from 3 healthy adults....
Embodiment 2
[0033] Preparation of gold nanoclusters
[0034] Prepare 1mmol / L HAuCl4 and 0.5mol / L NaOH solution. Add 10mg of GSH into 1mL of ultrapure water, stir in a water bath at 25°C until dissolved, quickly add 1mL of 1mmol / L HAuCl4 aqueous solution to the GSH solution under vigorous stirring and mix for 3 minutes, then add 0.5mol / L of NaOH solution 0.1 mL, stirred gently at 37°C for 10h.
[0035] Nanocluster Separation and Purification
[0036] The prepared nano-gold clusters were stored in the dark for 12 hours, and then poured into a 20000MWCO dialysis bag for dialysis for 12 hours (the water was changed every 4 hours). After dialysis, the mixed solution was filtered through a 0.22 μm microporous membrane with a syringe to remove excess impurities with large particle sizes. The finally obtained solution was stored at 0°C in the dark.
[0037] The GSH-AuNCs produced in Example 1 above. Blood samples were taken from 3 healthy adults.
[0038] experimental method:
[0039] Esta...
Embodiment 3
[0044] Preparation of gold nanoclusters
[0045] Prepare 10mmol / L HAuCl4 and 1mol / L NaOH solution. Add 125mg CEW to 2.5mL ultrapure water, stir in a water bath at 37°C until dissolved, quickly add 2.5mL 10mmol / L HAuCl4 aqueous solution to the CEW solution under vigorous stirring, mix for 5 minutes, then add 1mol / L NaOH solution 0.25mL, stirred gently at 37°C for 24h.
[0046] Nanocluster Separation and Purification
[0047] The prepared nano-gold clusters were stored in the dark for 24 hours, and then poured into a 20,000MWCO dialysis bag for dialysis for 24 hours (the water was changed every 8 hours). After dialysis, the mixed solution was filtered through a 0.22 μm microporous membrane with a syringe to remove excess impurities with large particle sizes. The finally obtained solution was stored at 4°C in the dark.
[0048] Experimental Materials
[0049] CEW-AuNCs produced in Examples 1, 2, and 3 above. Blood samples were taken from 3 healthy adults.
[0050] experime...
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