Application of acacetin extracted from Huai flos chrysanthemums in preparation of estrogenic medicines

Abstract

The invention relates to application of acacetin extracted from Huai flos chrysanthemums in preparation of estrogenic medicines, which aims at extracting acacetin form Huai flos chrysanthemums and preparing the estrogenic medicines by using the extracted acacetin. The acacetin of the flos chrysanthemums is obtained by the steps of performing tissue breaking on Huai flos chrysanthemums with acetonetwice, performing extraction twice, combining filtrates, and performing lower-temperature decompressed concentration to obtain a total extract; dispersing and dissolving the total extract by water, performing extraction with petroleum ether, ethyl acetate and n-butyl alcohol, taking dried ethyl acetate portions, performing dissolving with ethyl acetate, uniformly blending silica gel, then performing evaporation to dryness, performing silica gel column chromatography, performing spin drying on an eluent, performing dissolving in water, adding Toyopearl HW-40, performing gradient eluting with water and aqueous methanol in different proportions sequentially, sequentially treating the eluted part with sephadexLH-20, ODS, Silica gel and MCL Gel CHP-20 columns, and performing separation and purification in combination with methods for preparing high-efficient liquid phases and recrystallization. For the first time, the invention finds that the acacetin of the Huai flos chrysanthemum has anestrogen effect in bodies and plays a role in an estrogen receptor way, and a new purpose of the acacetin is opened up.

Description

technical field [0001] The invention relates to medicine, in particular to the application of acacetin extracted from Huai chrysanthemum in the preparation of estrogen-like drugs. Background technique [0002] Phytoestrogens exist in some natural plants and have estrogen-like effects. Phytoestrogens can bind to estrogen receptors and estrogen-related receptors in the body to regulate estrogen receptors bidirectionally, showing estrogen-like activity. Compared with synthetic estrogen, natural phytoestrogens have lower estrogen-like activity, but cause fewer side effects such as breast cancer and endometrial cancer, and have high safety. Therefore, the research on phytoestrogens has gradually become a hot spot. [0003] Huai chrysanthemum has high medicinal value, belongs to medicinal chrysanthemum, tastes sweet and bitter, cool in nature, contains about 0.13% volatile oil, inulin, adenine, choline, stachydrine, trace vitamin A-like substances, vitamin B, amino acids and lo...

AI Technical Summary

Problems solved by technology

However, there is no public report on proposing acacetin from Huai chrysanthemum and using it to prepare drugs with estrogen-like effects

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