8results about How to "Develop medicinal value" patented technology

The preparation method comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone compound extracted from epimedium sagittatum, thereby obtaining the isopentenyl flavone compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and two compounds are respectively obtained, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The preparation method of the isopentenyl flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, respectively extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone enantiomer compound, namely the isopentenyl flavone enantiomer compound. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

A method for extracting flavonoid compounds from epimedium sagittatum comprises the following steps: crushing epimedium sagittatum, carrying out reflux extraction, carrying out reduced pressure recovery to obtain an extract, suspending with distilled water, and extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol at room temperature to respectively obtain each part; the dichloromethane part is separated, eluted and combined, and components Fr.A, Fr.B, Fr.C, Fr.D, Fr.E, Fr.F, Fr.G and Fr.H are obtained; eluting the component Fr.G, and combining to obtain components Fr.G1,..., Fr.G17 and Fr.G18; eluting the component Fr.G17, and combining the eluted component Fr.G17 to obtain components Fr.G 17.1,..., Fr.G 17.15,..., Fr.G 17.19; eluting a component Fr.G 17.15, recovering under reduced pressure, and merging to obtain components Fr.G 17.15. 1,..., Fr.G 17.15. 9, Fr.G 17.15. 10,..., Fr.G 17.15. 12; the preparation method comprises the following steps: carrying out semi-preparative HPLC (High Performance Liquid Chromatography) purification on the component Fr.G 17.15. 10 to obtain a compound Epimesatine P; the preparation method comprises the following steps: carrying out semi-preparative HPLC (High Performance Liquid Chromatography) purification on a component Fr.G 17.15. 9, and collecting components with different retention times, so as to respectively obtain the compounds Epimesatine Q, Epimesatine R and Epimesatine S. The preparation method comprises the following steps of: preparing the compound Fr.G 17.15. 9; the flavonoid compound can be effectively extracted from epimedium sagittatum, can be effectively used for treating breast cancer, can be applied to preparation of anti-breast cancer drugs, and has remarkable economic and social benefits.

A preparation method of the triterpenoid A2 comprises the following steps: drying and crushing the oriental oriental waterplantain rhizome, dipping the oriental oriental waterplantain rhizome in acetone at room temperature, crushing and extracting, filtering, merging filtrate, and concentrating under reduced pressure to obtain an extract; sequentially extracting the extract with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol with the same amount, concentrating and drying to obtain a petroleum ether part, a dichloromethane part, an ethyl acetate part, an n-butyl alcohol part and a water part; dissolving the dichloromethane part with methanol, stirring silica gel, packing and eluting for four times to obtain a target fraction; and loading the target fractions on a chromatographic column, collecting fractions within the retention time, concentrating and drying to obtain the target fractions. The raw materials are rich, a unique preparation method is adopted, and the compound A2 can improve airway inflammation and airway hyperresponsiveness of OVA-induced allergic asthma, can be used for preparing drugs for treating allergic asthma, develops the medicinal value and economic value of oriental rhizoma alismatis, and has huge economic and social benefits.

The invention relates to a compound which is extracted from belladonna roots and has anti-inflammatory activity as well as a preparation method and application of the compound, the compound with a novel structure is found from the belladonna roots, the application problem of the novel compound in preparation of anti-inflammatory drugs is realized, and the technical scheme for solving the problem is that the compound is (2S)-1, 2-trigoyl-3-beta-D-glauronide-glycinol, the molecular formula is C19H27O11, the unsaturation degree is 6, 7, 8, 9, 10, 12, 13, 14, 16, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17, 17 According to the present invention, the raw materials are rich, the preparation method is easy to operate, the guidance quality is strong, the product purity is high, and the compound has significant inhibition effect on the generation of RAW264.7 cell nitric oxide with LPS-induced inflammatory reaction, has anti-inflammatory activity, can be effectively used for preparing anti-inflammatory drugs, develops the medicinal value of the belladonna root, and has significant economic and social benefits.

A method for extracting two tocopherol derivatives A and B from Epimedium sagittatum and application thereof can effectively solve the problem of preparing tocopherol derivatives A and B from Epimedium sagittatum and realize the application in preparing anti-breast tumor drugs. The method is as follows: the aboveground part of Epimedium sagittatum is crushed, extracted by refluxing with ethanol, recovered under reduced pressure, extracted, separated by chromatography, eluted by gradient, recovered under reduced pressure, identified by thin layer spotting, combined, purified, collected in the retention time, dried, and obtained. The raw material is abundant, the preparation method is easy to operate, the obtained product is good in quality and good in use effect, can be effectively used for treating breast cancer, realizes the application in preparing anti-breast cancer drugs, develops the medicinal value and commercial value of Epimedium sagittatum, and has remarkable economic and social benefits.

This invention relates to the field of medical pharmaceutical technology, and in particular to the application of peony oil in the preparation of a drug for treating benign prostatic hyperplasia (BPH). The preparation method of the peony oil includes the following steps: selecting peony plants, peeling peony seeds from the plants as raw materials, crushing and grinding the dried peony seeds using a crushing device to obtain granular raw materials, placing the granular raw materials in a container and adding an organic solution, soaking them in a cool place, then heating and refluxing for extraction, collecting the volatile oil to obtain peony oil, and filtering the peony oil through multiple layers to form peony oil. The application of this peony oil in the preparation of a drug for treating BPH utilizes abundant raw materials for peony oil extraction, and the preparation method is simple and low-cost. Peony flowers are used as the sole active ingredient in the extraction of peony oil, which can be effectively used to treat BPH, expanding the medicinal value of peony flowers and alleviating the suffering of patients during their illness.

The invention relates to a preparation method of anthriscus sylvestris lactone nanoparticles, which comprises the following steps: chopping dry roots of anthriscus sylvestris, extracting at room temperature to obtain an extract, suspending, and distributing by using petroleum ether, ethyl acetate and n-butyl alcohol respectively to obtain corresponding components; separating ethyl acetate components by silica gel column chromatography (CC), carrying out gradient elution, detecting and combining the components to respectively obtain subfractions, purifying the subfractions, eluting, and collecting fractions with retention time to obtain anthricin; preparing anthricin into a solution, and diluting; and dispersing, carrying out ultrasonic emulsification, stirring, centrifuging, removing unencapsulated medicines and impurities, and drying to obtain the anthricin nanoparticles. The method is novel and unique, anthriscus sylvestris lactone is effectively prepared from anthriscus sylvestris, the attenuated anthriscus sylvestris lactone nanoparticles are prepared through modification, and the attenuated anthriscus sylvestris lactone nanoparticles have an intervention effect on pulmonary arterial hypertension and can be used for preparing the medicine for treating pulmonary arterial hypertension, so that the medicinal value of anthriscus sylvestris and a new way for preparing the medicine for treating pulmonary arterial hypertension are developed, and the application prospect is wide. The medicine is an innovation of medicines for treating pulmonary arterial hypertension.