5results about How to "Low cost processing" patented technology

A microfluidic chip and detection method for nucleic acid amplification detection, the chip includes a base layer, a cover layer and a reversing valve, the cover layer is provided with an inlet, a purification and concentration chamber, a waste liquid outlet, a premixing chamber through hole and an outlet through hole, the inlet is connected to the waste liquid outlet and the premixing chamber through hole respectively after passing through the purification and concentration chamber through a flow channel, a porous material embedded with immunomagnetic beads is pre-set in the purification and concentration chamber, the reversing valve is arranged on the outlet side flow channel of the purification and concentration chamber to control the liquid in the purification and concentration chamber to flow to the waste liquid outlet or the premixing chamber through hole, the premixing chamber through hole is used to add reactants to mix with the liquid from the purification and concentration chamber, the base layer is provided with a mixing flow channel and a constant temperature amplification chamber with a transparent area, and the premixing chamber through hole is connected to the constant temperature amplification chamber through the mixing flow channel. The use of this chip can realize the purification, concentration, constant temperature amplification and real-time fluorescence detection of nucleic acids in one step, and obtain one or more target detection results quickly, simply and reliably.

To address the issues of insufficient performance and high cost associated with integral casting and additive manufacturing methods for parts with small-entry elliptical gradient deep cavities, and the difficulties, low efficiency, and high cost of metal removal methods, this invention proposes a machining method for elliptical gradient deep cavities with small entry points. The method involves first rough drilling and rough turning to obtain a deep hole. Then, a step-by-step machining approach is adopted, dividing the part into regions and machining them axially and radially. First, the part is tilted to machine the regions on both sides of the major axis of the ellipse. Then, the part is turned upright to machine the regions on both sides of the minor axis of the ellipse. During the upright machining, a radial layering and thin-cutting fast-running method is used. Several layers are machined using the first toolpath until the machining residue in the undercut and triangular regions is removed. Then, a second toolpath is used for contour machining to obtain a gradient deep cavity with an elliptical cross-section. Finally, the gradient deep cavity is precision milled until its internal dimensions and surface roughness meet the design requirements.

An asymmetric cross-shaped microstructure surface capable of regulating and controlling fluid multidirectional wetting comprises a substrate and a surface micro-texture on the substrate, each micro-texture unit comprises an asymmetric cross-shaped microstructure body, specifically comprises a cross-shaped body and pointed tails in different directions, is of a relatively simple plane structure and is convenient to manufacture on a large scale; and the plurality of asymmetric cross-shaped microstructure bodies are arranged in a linear array. A plurality of narrow channels with sharp edges are formed by the sharp tail parts of every two adjacent asymmetric cross-shaped microstructures which are reasonably arranged, and the structures play a role in hindering or promoting fluid guiding in different directions, so that pinning, one-way, two-way, three-way or four-way wettability regulation and control of fluid are achieved, and meanwhile, the structure is simple, and low-cost machining is facilitated.

The utility model provides a large-specification stainless steel pipe fitting outer circle machining tool which comprises a lead screw, a fixed end cover and a locking nut. Central holes are formed in two ends of the screw rod body; the length of the lead screw body is larger than the axial length of the workpiece, and the two ends of the lead screw body extend out of the two ends of the workpiece. The two ends of the workpiece are concentrically positioned and adaptively provided with the fixed end covers, end cover center holes formed in the fixed end covers and the lead screw are concentrically positioned and adaptively installed, the end cover center holes transfer the center of the workpiece to the centers of the fixed end covers, and the outer threads at the two ends of the lead screw are screwed and adaptively provided with the locking nuts which clamp the fixed end covers of the workpiece. Therefore, the workpiece and the lead screw are concentrically locked, fastened and connected into a whole. According to the utility model, the technical problem that a large-specification stainless steel pipe fitting workpiece cannot be clamped and machined on an ME1332A cylindrical grinding machine at high precision is solved, the machining precision grade can reach IT5 and IT7, the surface roughness is less than 0.2 mu m, and high-precision, high-efficiency and low-cost machining of the outer circle of the large-specification stainless steel pipe fitting workpiece is realized.

The utility model discloses a modular planting flower box based on metal plate buckle assembly, belongs to the technical field of metal gardening facilities, and realizes welding-free and screw-free quick assembly through a U-shaped clamping groove and an elastic locking mechanism which are formed by precise bending. The innovatively-designed four-layer composite stand column structure provides the bearing capacity larger than or equal to 150 kg / m, and the volume compression rate after folding reaches 85%. The method is suitable for various scenes such as household gardening and municipal greening.