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Denitrification acinetobacters and use thereof

A bacillus, denitrification technology, applied in the field of denitrification Acinetobacter and its application

Inactive Publication Date: 2012-10-24
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The denitrification method of aureomycin-containing sewage mainly adopts traditional aerobic and anaerobic graded treatment, but the application of SND denitrification method to remove nitrogen in aureomycin-containing sewage has not been reported yet.

Method used

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  • Denitrification acinetobacters and use thereof
  • Denitrification acinetobacters and use thereof
  • Denitrification acinetobacters and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The screening of embodiment 1 LN5 bacterial strain

[0047] 1. Separation

[0048] Collect nitrogen-containing sewage samples containing aureomycin from the environment, and dilute them by volume concentration gradient for 10 6 、10 7 、10 8 、10 9 and 10 10 times, the diluted samples were evenly spread on the bromothymol blue (BTB) solid medium plate, and placed in a 37 ° C incubator for 2 days to obtain a blue halo on the BTB solid medium plate Circle of colonies.

[0049] The BTB solid medium ( / L): agar 10g, KNO 3 1g, KH 2 PO 4 1g, FeCl 3 ·6H 2 O0.5g, CaCl 2 ·7H 2 O 0.2g, MgSO 4 ·7H 2 O 1g, sodium succinate 8.5g, BTB ethanol solution (0.1g bromothymol blue dissolved in 10mL ethanol) 1mL, dilute to volume with distilled water, initial pH value is 7.0, sterilize at 121°C for 20min to make BTB solid medium flat.

[0050] 2. Purification

[0051] Pick each single colony showing a blue halo from the cultured BTB solid medium plate, and streak continuously on...

Embodiment 2

[0068] Example 2 Identification of Morphological Characteristics and Physiological and Biochemical Properties of LN5 Bacterial Strain

[0069] 1. Identification of Morphological Characters

[0070] The colony and microscope morphology observations of the LN5 strain are as follows:

[0071] The LN5 strain was inoculated in beef extract peptone liquid medium for activation for 24 h, and then diluted to 10 with sterile water 9 Double-fold and evenly spread on the beef extract peptone solid medium plate, put it in a constant temperature incubator at 37°C for 24 hours, observe and obtain the morphological characteristics of the colony as follows: the colony is round, small and raised, with smooth surface, neat edges and moist , white opaque, easy to pick, such as figure 1 shown.

[0072] Activate the LN5 strain in beef extract peptone liquid medium for 24 hours, then dilute it 100 times with sterile water, drop 1 drop of the diluted bacterial solution on a clean glass slide, and...

Embodiment 3

[0082] Example 3 Molecular biological identification of LN5 bacterial strain

[0083] 1. Genomic DNA Extraction

[0084] ① Bacteria culture: inoculate LN5 strain in 50ml beef extract peptone liquid medium, culture at 30℃, 220rpm for 24h, take 5ml of bacterial suspension at 10000rpm, centrifuge for 1min, collect the bacteria in a 1.5mL centrifuge tube .

[0085] ② Genomic DNA extraction: Genomic DNA of LN5 strain was extracted using the Bacterial Genomic DNA Extraction Kit produced by Tiangen Biochemical Technology Co., Ltd.

[0086] ③Preservation: Store the extracted genomic DNA of the LN5 strain at -20°C.

[0087] ④ Detection: After the extracted genomic DNA of the LN5 strain was subjected to 1% agarose gel electrophoresis, it was observed and photographed in a gel imaging system, and the electrophoresis image was obtained as follows: image 3 As shown, the M lane is Marker, and the 1 and 2 lanes are electrophoresis products.

[0088] 2. PCR Amplification and Detection of...

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Abstract

The invention relates to denitrification acinetobacters and a use thereof, and belongs to the field of environmental microbiology. The denitrification acinetobacters have a category name of Acinetobacter schindleri LN5, are preserved in the China general microbiological culture collection center of the China microbiological preservation management committee on May 30th, 2012, and has a preservation number of CGMCC No.6165. The denitrification acinetobacters have heterotrophic nitrification-aerobic denitrification functions, have high aureomycin resistance, can be used for simultaneous nitrification and denitrification (SND) treatment on nitrogen-containing sewage and especially on nitrogen and aureomycin-containing sewage, realize removal of a large part of ammonia nitrogen in nitrogen-containing sewage, realize small accumulation amounts of nitrite and nitrate nitrogen, realize nitrification-denitrification and COD removal in the same reactor, and realize environmentally-friendly, low-consumption and high-efficiency treatment on nitrogen-containing sewage.

Description

technical field [0001] The present invention relates to a denitrification Acinetobacter and its application, in particular to a denitrification Acinetobacter LN5 (Acinetobacter schindleri LN5), the denitrification Acinetobacter LN5 has good tolerance to aureomycin ability, and has the function of heterotrophic nitrification-aerobic denitrification, which can be used for biological denitrification treatment of nitrogen-containing sewage and aureomycin-containing sewage, and belongs to the field of environmental microorganisms. Background technique [0002] Nitrogen is one of the main factors causing eutrophication of water bodies, and the substandard discharge of nitrogen-containing sewage aggravates the eutrophication of water bodies. Nitrogen removal methods in sewage are divided into physical and chemical denitrification methods and biological denitrification methods. Physicochemical denitrification method is expensive and difficult to popularize and apply. Biological de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 李艳菊袁聪颖梁凌飞董晓霞
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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