The invention discloses a novel PLA cotton blended fabric. The novel PLA cotton blended fabric is prepared through the steps of 1 preprocessing; 2, complex enzyme processing, wherein after preprocessing is performed, the polylactic acid/cotton blended fabric is subjected to dip processing in a complex enzyme, and according to the enzyme processing technological conditions, bromelain, phytase and catechol oxidase mixed processing is adopted; 3, aicd dyeing, wherein the polylactic acid/cotton blended fabric obtained after enzyme processing is performed in the step 2 is subjected to dyeing processing through acid dyes, and according to the dye liquor formula and conditions, the concentration of the acid dyes is 2-3 g/L, the concentration of anhydrous sodium sulphate is 1-2 g/L, peregal O/%(omf) is 0.3%, pH is 4, the temperature is kept to be 50 DEG C, the temperature is increased to 70-80 DEG C after 10 min and kept constant for 60-70 min, and the bath ratio is 1:25; 4, processing performed after washing, wherein the polylactic acid/cotton blended fabric dyed in the step 3 is washed under the temperature of 60-80 DEG C for 5-20 min, and the bath ratio is 1:(5-30).