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Extraction process of tea polysaccharide

An extraction process and technology of tea polysaccharides, applied in the direction of anti-toxic agents, drug combinations, etc., can solve the problems of reducing bioavailability, inactivation of tea polysaccharides, easy to destroy polysaccharide structures, etc., to improve yield and antioxidant activity, and promote dissolution , The effect of shortening the extraction time

Active Publication Date: 2015-11-18
GUANGXI DONGXING DINGKANG PLASTIC IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional tea polysaccharides are extracted using cold or warm water, polysaccharides are difficult to diffuse out of the cells, high temperature can easily inactivate tea polysaccharides, and acid and alkali methods can easily destroy the structure of polysaccharides and reduce their bioavailability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A process for extracting tea polysaccharides, comprising the following steps:

[0026] (1) Pretreatment: first wash the fresh tea leaves with clear water, then send the fresh tea leaves into a microwave vacuum dryer, control the pressure of the microwave vacuum dryer to 12KPa, and the temperature is 60°C, and dry the fresh tea leaves until the moisture content is 4%, then the tea leaves are pulverized to a fine powder of 60 meshes to obtain tea powder;

[0027] (2) Enzymolysis: add water to tea powder, the weight ratio of fresh tea leaves and water is 1:6, and add hydrochloric acid to adjust the pH value of the solution to 7, then add cellulase and polyphenol oxidase, polyphenol oxidase The weight ratio of polyphenol oxidase and cellulase is 1:8, and the total amount of polyphenol oxidase and cellulase is 0.05% of the solution, and hydrolyzed at 60°C for 60 minutes to obtain an enzymatic hydrolyzate;

[0028] (3) Ultrasonic treatment: send the enzymatic hydrolysis solu...

Embodiment 2

[0035] A process for extracting tea polysaccharides, comprising the following steps:

[0036] (1) Pretreatment: first wash the fresh tea leaves with clear water, then send the fresh tea leaves into a microwave vacuum dryer, control the pressure of the microwave vacuum dryer to 24KPa, and the temperature is 40°C, and dry the fresh tea leaves until the moisture content is 6%, then the tea leaves are pulverized to fineness of 200 mesh fine powder to obtain tea powder;

[0037] (2) Enzymolysis: add water to tea powder, the weight ratio of fresh tea leaves and water is 1:6, and add hydrochloric acid to adjust the pH value of the solution to 6.2, then add cellulase and polyphenol oxidase, polyphenol oxidase The weight ratio of polyphenol oxidase and cellulase is 1:8, and the total amount of polyphenol oxidase and cellulase is 0.03% of the solution, and hydrolyzed at 40°C for 40 minutes to obtain an enzymatic hydrolyzate;

[0038] (3) Ultrasonic treatment: send the enzymatic hydroly...

Embodiment 3

[0045] A process for extracting tea polysaccharides, comprising the following steps:

[0046] (1) Pretreatment: first wash the fresh tea leaves with clear water, then send the fresh tea leaves into a microwave vacuum dryer, control the pressure of the microwave vacuum dryer to 16KPa, and the temperature is 50°C, and dry the fresh tea leaves until the moisture content is 5%, then the tea leaves are pulverized to a fine powder with a fineness of 100 mesh to obtain tea powder;

[0047](2) Enzymolysis: add water to tea powder, the weight ratio of fresh tea leaves and water is 1:6, and add hydrochloric acid to adjust the pH value of the solution to 6.5, then add cellulase and polyphenol oxidase, polyphenol oxidase The weight ratio of polyphenol oxidase and cellulase is 1:7, and the total amount of polyphenol oxidase and cellulase is 0.04% of the solution, hydrolyze at a temperature of 50°C for 50 minutes, and obtain an enzymatic hydrolyzate;

[0048] (3) Ultrasonic treatment: send...

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PUM

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Abstract

The invention discloses an extraction process of tea polysaccharide. The process includes: firstly washing fresh tea leaves with clean water, then performing microwave vacuum drying, then crushing the tea leaves, adding water into the obtained tea powder, and adding acid to adjust the pH value of the solution, then adding cellulase and polyphenol oxidase, carrying out hydrolysis under certain temperature, sending the obtained enzymolysis liquid into ultrasonic equipment to conduct ultrasonic treatment, then performing filtering and sending the filtrate into ultrafiltration equipment to conduct ultrafiltration treatment, then putting the ultrafiltrate into a centrifugal machine to conduct centrifugal separation, concentrating the obtained centrifugate, adding ethanol into the concentrated solution, carrying out centrifugal separation, and drying the tea polysaccharide, thus obtaining tea polysaccharide. By adopting the microwave low temperature vacuum drying and ultrasonic wave synergistic treatment technology, the process provided by the invention ensures effective wall breaking, and significantly improves the yield and antioxidant activity of polysaccharide.

Description

technical field [0001] The invention relates to the technical field of sugar processing technology, in particular to a tea polysaccharide extraction technology. Background technique [0002] Tea polysaccharide is an acidic glycoprotein combined with a large amount of mineral elements, called tea polysaccharide complex, referred to as tea polysaccharide or tea polysaccharide (TeaPolysaccharide). Among them, the protein part is mainly composed of about 20 kinds of common amino acids, the sugar part is mainly composed of arabinose, xylose, fucose, glucose, galactose, etc., and the mineral elements are mainly composed of calcium, magnesium, iron, manganese, etc. and a small amount of trace elements, such as rare earth elements. It has the effects of lowering blood sugar, lowering blood fat, enhancing immunity, lowering blood pressure, slowing heart rate, increasing coronary flow, anticoagulant, antithrombotic and hypoxia resistance. In recent years, tea polysaccharides have bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
Inventor 邱峰
Owner GUANGXI DONGXING DINGKANG PLASTIC IND
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