Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone
A technology of pueraria starch and isoflavones, which is applied in the direction of fermentation, can solve the problems of affecting product quality, phenolic substances are prone to browning, difficulty, etc., and achieves the effects of improving the utilization rate of raw materials, avoiding the phenomenon of browning, and promoting rapid degradation.
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Embodiment 1
[0028] The process for efficiently extracting Pueraria starch and Pueraria total isoflavones of the present embodiment comprises the following steps:
[0029] a. After mixing 10 kg of kudzu root with 20 kg of water, carry out initial crushing and filing to obtain the mixed material, then apply a high pressure of 130 MPa to the mixed material, and the pressure application time is 10 minutes. After testing, after applying the high pressure, The activity of polyphenol oxidase in the mixed material was reduced to 34%;
[0030] b. adjust the temperature of the mixed material obtained in step a to 33-35° C. and add cellulase, and after 1 hour, file the mixed material again to obtain a slurry mixture, and the weight of cellulase is 1% of the weight of the mixed material;
[0031] c. adjust the pH value of the slurry mixture obtained in step b to be 8, adjust the pH value to 5 after 1 hour, and separate the slurry mixture after 0.5 hour to obtain slurry and kudzu root residue;
[003...
Embodiment 2
[0041] The process of efficiently extracting Pueraria starch and Pueraria total isoflavones of the present embodiment: comprises the following steps:
[0042] a. After mixing 10 kg of kudzu root with 30 kg of water, carry out initial crushing and filing to obtain the mixed material, then apply high pressure to the mixed material, the size of the high pressure is 300MPa, and the number of times of application is 2 times, each 15mins, After an interval of 3 minutes, after applying high pressure, the activity of polyphenol oxidase in the mixed material was detected to be reduced to 28% of the original;
[0043] b. adjust the temperature of the mixed material obtained in step a to 35-38° C. and add cellulase. After 1.5 hours, the mixed material is again ground to obtain a slurry mixture. The weight of the cellulase is the weight of the mixed material 3%;
[0044] c. adjust the pH value of the slurry mixture obtained in step b to be 9, adjust the pH value to 6 after 1.5 hours, and...
Embodiment 3
[0054] The difference between the technology of the present embodiment and embodiment 2 is:
[0055] In step a, the high pressure is 260MPa, and the number of times of application is 3 times, 20 minutes each time, with an interval of 3 minutes in between. After the high pressure is applied, the activity of polyphenol oxidase in the mixed material is reduced to 15% of its original value;
[0056] In step b, the temperature of the mixed material is adjusted to 33-36°C, and the weight of cellulase is 2% of the weight of the mixed material;
[0057] In step c, the pH adjustment method is: first adjust the pH value of the slurry mixture to 8, and then adjust the pH value to 5 after 1.5 hours;
[0058] In step d, the weight of water added to the slurry obtained in step c is 2 times of its own weight;
[0059] In step i, pueraria slag is immersed in 3 times the weight of ethanol solution and subjected to intermittent microwave treatment at 65° C., the mass percentage of ethanol in t...
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