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Method and apparatus for measuring methanol concentration in alcohols solution

A measuring device, methanol concentration technology, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems that cannot be used in the screening of counterfeit wine, cannot distinguish the coexistence of methyl alcohol and ethanol, and achieve an improvement Convenience, the effect of avoiding poisoning incidents

Inactive Publication Date: 2008-12-24
DEV CENT FOR BIOTECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is that alcohol oxidase can not only act on methanol, but also oxidize ethanol at the same time, that is, it cannot distinguish the coexistence of formazan and ethanol, so it cannot be used for the screening of fake wine

Method used

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  • Method and apparatus for measuring methanol concentration in alcohols solution
  • Method and apparatus for measuring methanol concentration in alcohols solution
  • Method and apparatus for measuring methanol concentration in alcohols solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 SPE electrode preparation and enzyme immobilization

[0047] In terms of electrode preparation, electrode materials that can be used in the present invention include: indium oxide, glass, gold, platinum, palladium, graphite, and carbon black. In terms of electrode structure, no matter it is a flat plate electrode, a solid acicular electrode or a hollow acicular electrode, as long as it can achieve the purpose of the present invention without bad results, it can be applied to the present invention; on the electrode surface treatment, preferably with acid, Alkali, physical grinding or ultrasonic treatment to get a clean electrode surface. Embodiment 1 of this study uses a screen printing electrode (SPE) as an exemplary embodiment.

[0048] The SPE electrode used in this study was purchased from Taibo (Qiaoying) Technology Co., Ltd., and its structure is shown in Figure 2. The SPE electrode 200 comprises a substrate 202 of PVC material; a reference electrode 20...

Embodiment 2

[0051] Example 2 Effect of MB High Water Solubility and MB-RS Ratio on Current Output Signal

[0052] FIG. 3A is a situation of continuous measurement of methanol content using SPE electrodes for a fixed concentration of methanol according to the present invention. It can be clearly seen in Figure 3A that the oxidation current signal continued to decrease as the number of measurements increased, and at the same time the color of the solution in the measuring tube gradually changed from colorless to blue, that is, the color presented by MB dissolved in the measuring solution. These results show that MB has high water solubility and has a great influence on the stability of the electrode.

[0053] In order to overcome the problem of high water solubility of MB, the present invention mixes 0.1M MB with 0.1M Reinecke salt to react to generate MB-RS complex precipitates with low water solubility, which are collected by centrifugation Finally, after drying for about 30 minutes, gri...

Embodiment 3

[0055] Example 3 Optimum ratio of enzymes

[0056] In this example, for electrodes prepared with different ratios of AOX / FDH, in the same NAD + The current output signal was measured under the condition of methanol concentration, and the results are shown in Figure 5. The current output signal 502 has a volume ratio of AOX:FDH of 1:20. The current output signal 504 has a volume ratio of AOX:FDH of 1:10. The current output signal 506 has a volume ratio of AOX:FDH of 1:5. The current output signal 508 has a volume ratio of AOX:FDH of 1:1. In this example NAD + The added amount is 1.3 mg, and the active concentration of AOX is about 1020 U / ml, and the active concentration of FDH is about 550 U / ml. The active concentrations of different volume ratios are listed in Table 1. When the activity ratio of AOX:FDH is between about 1U:0.5-11U, the claimed effect of the present invention can be effectively achieved. It should be noted that even when the activity ratio of AOX:FDH is a...

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Abstract

The invention provides a method and device used for measuring carbinol content in alcoholic solutions. The device of the invention is a biological micro-sensor which is used for electrochemical carbinol measurement and based on enzyme reaction, can carry out quick and sensitive detection aiming at the content of the carbinol in real alcohol samples, achieve the effect of saving time and cost, and greatly improve the convenience in use. The method and device of the invention can be directly used for the screening and checking of the false wine, thus achieving the aim of avoiding the generation of false wine poisoning events.

Description

【Technical field】 [0001] The invention relates to a method and a device for detecting methanol content in alcohol solutions, and in particular to a method and a device for detecting methanol content in alcohol solutions using a dual-enzyme system. 【Background technique】 [0002] Incidents of counterfeit wine poisoning have been heard from time to time. The main reason is that the wine is mixed with high concentrations of methanol, so that some people become blind or even die after drinking it. Methanol, also known as wood spirit or industrial alcohol, is often used as a solvent or in many other industrial applications; methanol can also be used as fuel, making moonshine, paint remover and denaturant, but real wine also contains trace amounts of methanol. [0003] Methanol is gradually oxidized to formaldehyde and formic acid in the liver due to the catalytic reaction of alcohol dehydrogenase. However, the toxicity of formaldehyde is about 33 times that of methanol, and form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416
Inventor 巫鸿章钱瑞龙陈建孝林毕修平
Owner DEV CENT FOR BIOTECHNOLOGY
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