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Method for detecting adrenaline by metal-terbium fluorescent probe and use of method

An epinephrine and probe detection technology, used in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., can solve the problems of poor stability modified electrodes, short electrode service life, etc., to achieve an effective detection method, improve stability performance, convenient for measuring the effect of operation

Inactive Publication Date: 2017-05-31
JIANGSU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the most reported electrochemical analysis method is the chemically modified electrode method. This method has poor stability and short service life for the determination of biological samples. The method needs to be further improved.

Method used

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  • Method for detecting adrenaline by metal-terbium fluorescent probe and use of method
  • Method for detecting adrenaline by metal-terbium fluorescent probe and use of method
  • Method for detecting adrenaline by metal-terbium fluorescent probe and use of method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Fluorescence detection of epinephrine ternary complex

[0036] 1. Solution preparation

[0037] (1) Tb(III)-EDTA stock solution (0.05mol / L): Accurately weigh 1.8612g Na 2 EDTA·2H 2 O in a 100mL beaker, add 2mL0.1mol / L NaOH, add water to dissolve, add 1.5966g TbCl 3 2H 2 O, after dissolving, transfer to a 100mL volumetric flask and dilute to the scale constant volume. When used, it was diluted step by step according to the required concentration.

[0038] (2) Epinephrine stock solution (1.0×10 -3 mol / L): Accurately weigh 0.0183g of epinephrine standard substance (Nanjing Drug Control Institute) into a 50mL beaker, add 5mL of 0.1mol / L HCl, add a small amount of water and 1mL of 4.0×10 -3 mol / LNa 2 SO 3 , and then transferred to a 100mL volumetric flask to dilute to the volume and store in the refrigerator. When used, it was diluted step by step according to the required concentration.

[0039] 2. Fluorescence Assay

[0040] Take three 10mL colorimetric tubes and ...

Embodiment 2

[0046] Optimization of Experimental Conditions, Establishment of Fluorescence Analysis Method for Determination of Epinephrine

[0047] (1) Solution acidity optimization

[0048] Solution acidity is the key factor for forming ternary complexes, and the optimization of solution acidity follows the steps below:

[0049] In nine 10mL colorimetric tubes, add 1mL 5.0×10 -5 mol / L epinephrine solution, 1mL 1.0×10 -2 mol / L Tb(III)-EDTA solution, a certain amount of Tris buffer solution and adjusted to the following required pH values ​​with dilute hydrochloric acid and sodium hydroxide: 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, 13, Finally, add 2 mL of 1.0×10 -3 mol / LCTAC, dilute to the mark; prepare complex solutions with pH values ​​of 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, and 13 different acidities, and then use a 1cm cuvette at an excitation wavelength of 302nm, the emission wavelength is 545nm to measure the fluorescence intensity of the system. The acidity corresponding to the ...

Embodiment 3

[0058] Selectivity of Epinephrine Assay

[0059] Add the solution in a 10mL colorimetric tube as follows: 1mL 5.0×10 -5 mol / L epinephrine standard solution, 1mL 1.0×10 -2 mol / L Tb(III)-EDTA solution, 1mL0.1mol / L NaOH solution, 2mL 1.0×10 -3 mol / LCTAC and a certain concentration of an interfering substance, diluted to the mark;

[0060] Shake well, and measure the relative fluorescence intensity after standing for 20 min. Compared with no interfering substances, determine whether the interfering substances interfere with the change of the fluorescence intensity of the system not exceeding ±5%. According to the above-mentioned experimental method, the interference of common metal ions and pharmaceutical excipients in the determination of epinephrine was experimentally analyzed, and the determination results are shown in Table 1.

[0061] The impact of table 1 interfering substances on the determination of epinephrine (C E =5.0×10 -6 mol / L)

[0062]

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Abstract

The invention relates to a method for detecting adrenaline by a metal-terbium fluorescent probe and use of the method. According to the method, the adrenaline, disodium edetate and a rare-earth element terbium form a stable ternary complex in an alkaline solution, and the ternary complex can emit characteristic fluorescence of Tb(III). Through adding hexadecyltrimethylammonium chloride, which serves as a sensitizing agent, into the ternary complex, the fluorescence intensity of the ternary complex can be remarkably enhanced. The relative fluorescence intensity of the ternary complex is proportional to adrenaline concentration in the adrenaline concentration range of 7.0*10<-8>mol / L to 5.0*10<-5>mol / L, and the detection limit is 2.1*10<-8>mol / L. According to the method, the interference resulting from common metal ions and drug excipients can be eliminated; the method is high in sensitivity, simple in operation and low in detection limit; the result is good when the method is applied to the detection on adrenaline injections.

Description

technical field [0001] The invention relates to the technical fields of drug analysis and biological analysis, in particular to a method for detecting adrenaline with a metal terbium fluorescent probe and its application. Background technique [0002] Adrenaline (E) is an important neurotransmitter in the central nervous system of mammals (including humans), and it is one of the naturally occurring catecholamines. Its concentration in the body affects a variety of physiological processes, and its metabolic disorder can lead to occurrence of certain diseases. Epinephrine hydrochloride drugs are commonly used in medicine to treat acute heart failure, anaphylactic shock, asthma, nasal congestion, hypotension and other diseases. Epinephrine was the first hormone to obtain a crystalline form. Since adrenaline has a catechol structure, it has a strong reducing property, is easily oxidized in air or aqueous solution, and has poor stability. In the human body, epinephrine is form...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 连宁李蕾刘丹王欣张国华
Owner JIANGSU UNIV OF TECH
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