Method for detecting phthalic acid ester compound concentration based on optical fiber immunosense

A technology of phthalic acid and ester compounds, applied in measuring devices, fluorescence/phosphorescence, and material analysis through optical means, can solve the problem of low sensitivity, inability to simultaneously detect multiple PAEs, and narrow linear range of immunoassay methods and other issues to achieve the effect of wide recognition range

Inactive Publication Date: 2016-03-23
SOUTHWEST UNIVERSITY
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Problems solved by technology

[0004] The existing immunoassay methods have a narrow linear range and low sensitivity, and the prepared antibod

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  • Method for detecting phthalic acid ester compound concentration based on optical fiber immunosense
  • Method for detecting phthalic acid ester compound concentration based on optical fiber immunosense
  • Method for detecting phthalic acid ester compound concentration based on optical fiber immunosense

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Embodiment Construction

[0030]At present, most of the ELISA methods can only detect a single PAEs, and there are no research reports on the simultaneous identification and rapid detection of multiple PAEs. For this reason, the present invention utilizes PAEs universal hapten and specific antibody to establish a method for detecting the concentration of phthalates based on optical fiber immunosensing. The specific processing steps are as follows.

[0031] 1) Construction of optical fiber immune sensing detection system;

[0032] The optical fiber immunosensing detection system constructed by the present invention includes a laser emitting device, an asymmetric optical fiber coupler for laser transmission and fluorescence reception, an optical fiber probe, a sample pool, a fluorescence filter, a photodiode, a lock-in amplifier and a computer, and an asymmetric optical fiber Couplers consist of single-mode fiber and multimode fiber. One end of the multimode fiber of the asymmetric fiber coupler is con...

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Abstract

The invention discloses a method for detecting the phthalic acid ester compound concentration based on optical fiber immunosense. The method includes the steps that firstly, an optical fiber immunosense detection system is established; secondly, a standard inhibition curve is established, wherein (2.1), a fluorescently-labeled antibody solution and a phthalic acid ester compound standard solution with the known concentration are mixed, data obtained after pre-reaction are input to the optical fiber immunosense detection system, and a response signal is obtained; (2.2), the concentration of phthalic acid ester compounds is changed, the step of (2.1) is repeated, and response signals under different concentrations are obtained; (2.3) the standard inhibition curve is drawn according to the concentration logarithm value and the inhibition ratio; (3.1), the step of (2.1) is repeated on the phthalic acid ester compounds to be detected, and a response signal and the inhibition ratio are obtained; (3.2) according to the standard inhibition curve, the concentration of the phthalic acid ester compounds to be detected can be obtained. The method can detect various kinds of PAEs at the same time and is high in sensitivity and precision and fast in detection.

Description

technical field [0001] The invention relates to the detection of phthalate compounds, in particular to a method for detecting the concentration of phthalate compounds based on optical fiber immune sensing, and belongs to the technical field of biological detection. Background technique [0002] Phthalate esters (phthalateesters, PAEs), also known as phthalates, are widely used as plasticizers in industrial production and plastic products, which can improve the plasticity and strength of plastic products. PAEs are generally colorless and transparent oily viscous liquids, hardly soluble in water, easily soluble in organic solvents such as methanol, ethanol, and ether, and belong to low-volatility, medium-polarity, and high fat-soluble substances. In plastic products, PAEs have good compatibility with plastic molecules, and the two cannot form effective chemical bonds, but are connected by hydrogen bonds or van der Waals forces, maintaining their independent chemical properti...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 张进忠吴永烽汤民邓冬莉杨大成彭远义
Owner SOUTHWEST UNIVERSITY
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