The invention belongs to the technical field of organic substance extraction, and discloses a novel extraction method of total flavonoids in Senecio scandens. The method comprises the steps of conducting cleaning, disinfection, drying and pulverizing on the above part of fresh Senecio scandens root, conducting sieving by a 20 mesh sieve to obtain a Senecio scandens powder; putting the Senecio scandens powder in a 60% ethanol solution, conducting immersing for 1 hour, and conducting ultrasonic extraction for 40 min under conditions of 350 W and 80 DEG C; after ultrasonication, taking out the solution, conducting suction filtration, retaining the filtrate, immersing the filter residue in 70% ethanol for 1 hour and then conducting vacuum suction filtration; merging the two filtrates, conducting centrifugation to obtain a supernatant, conducting concentration to volatilize off remaining ethanol to obtain a flavonoid extract concentrate; and conducting lyophilization to obtain a lyophilizedpowder, that is, the total flavonoids in Senecio scandens. The method of the invention adopts an ultrasonic extraction method to extract total flavonoids in Senecio scandens, the detection process issimple, the extraction rate is high, the extraction speed is fast, and the problem of long-time reflux extraction in a traditional extraction process is avoided.