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Staining agent combination for tumor tissue cell and preparation method thereof

A tumor tissue and tumor cell technology, applied in the preparation of test samples, organic dyes, etc., can solve the problems of difficult to achieve methylene blue reduction, unstable color change, etc., achieve simple positioning and detection, short inspection time, and facilitate promotion Effect

Active Publication Date: 2014-01-01
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our research found that under the experimental conditions, sugar is a weak reducing agent, and it is difficult to achieve the reduction of methylene blue; using weakly alkaline conditions to prepare reduced methylene blue, when adding acidic reagents, such as acetic acid, reduced methylene blue will show Blue, which in turn leads to unstable color changes when dyeing

Method used

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  • Staining agent combination for tumor tissue cell and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolving medical-grade folic acid in an aqueous solvent, adding the chromogenic agent methylene blue and stirring to dissolve, adding the reducing agent ascorbic acid, stirring for 1 hour, adding analytically pure acetic acid, stirring and mixing to prepare a dye composition. Dip the detection agent composition with a large cotton swab, apply it evenly on the surface of the tissue, and observe the color of the cotton swab and the color change of the tissue.

Embodiment 2

[0022] Dissolving medical-grade folic acid in an aqueous solvent, adding the color developing agent methylene blue and stirring to dissolve, adding the reducing agent zinc powder, stirring for 1 hour, adding analytically pure acetic acid, stirring and mixing to prepare a dye composition. Dip the detection agent composition with a large cotton swab, apply it evenly on the surface of the tissue, and observe the color of the cotton swab and the color change of the tissue.

Embodiment 3

[0024] Repeat the method of Example 1 according to the percentage content of each component specified in Table 1 to make a dye composition. Dip the detection agent composition with a large cotton swab, apply it evenly on the surface of the tissue, and observe the color of the cotton swab and the color change of the tissue.

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Abstract

The invention relates to a staining agent combination for tumor tissue cells, in particular to a staining agent combination for the tumor tissue cells. The combination comprises 0.01%-5% of a color developing agent, less than or equal to 6.5% of folic acid, 0.1%-10% of a leuco agent, less than or equal to 10% of a pH modifier and 3%-95% of water, wherein the color developing agent adopts methylene blue and / or dichlorophenolindophenol; the leuco agent adopts a reducing reagent; a targeted drug preparation adopts the folic acid; and the solution pH modifier and the water are adopted. The coloring agent for staining tumor cell can also be used for detecting the tumor cells. The invention also relates to a method of the staining agent combination used for detecting the tumor cells, a kit for detecting the tumor cells and a detection carrier.

Description

technical field [0001] The invention relates to a stain composition for tumor tissue cells, in particular to a color change-based stain composition for tumor cells. The present invention also relates to the composition used for detecting tumor cells and its carrier. technical background [0002] At present, two kinds of dyes for tumor cells are commonly used in the world, Ji's and Wright's dyes. They are mixtures of acid-base chemicals after heating, and their ratios are likely to be out of balance due to humidity and other reasons. In addition, their effect on Cell staining is not specific. [0003] The methods used to stain tissue tumor cells in vivo mainly include acetic acid whitening reaction, iodine experiment, toluidine blue experiment, and methylene blue experiment, among which methylene blue staining is more extensive. During the acetic acid whitening reaction, apply 3-5% acetic acid solution to the tissue cells, and wait for a period of time to observe whether th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B67/42C09B67/22G01N1/30
Inventor 李建忠单虎
Owner QINGDAO AGRI UNIV
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