ELISA reagent kit for testing Asial type foot-and-mouth disease virus

The technology of a foot-and-mouth disease virus and a kit is applied in the field of ELISA kit and preparation for detecting Asia1 type foot-and-mouth disease virus, which can solve the problems of long duration and complicated epidemic situation, and achieve the effect of simple detection process and preparation process.

Inactive Publication Date: 2007-12-05
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The outbreak not only lasted for a long time, but also was complicated

Method used

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  • ELISA reagent kit for testing Asial type foot-and-mouth disease virus
  • ELISA reagent kit for testing Asial type foot-and-mouth disease virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] 1. Preparation of monoclonal antibody

[0046] (1) Use the RNeasy Mini Kit (Qiagen) kit to extract the total mRNA from the Asia1 type foot-and-mouth disease virus (note: the strain is stored in the National Foot-and-Mouth Disease Reference Laboratory of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) as a template, and use the Asia1 type The specific primers of VP1 were reverse-transcribed and PCR-amplified to obtain the VP1 gene of Asia1 type foot-and-mouth disease virus. The primers used in the present invention are:

[0047] Upstream primer 5′-CCG GAATTC GCTTACCAGTGGATGCTCG-3′

[0048] Downstream primer 5-′CCC AAGCTT CGCTAGCTTGAGCAGGTC-3′

[0049] Transform the ligation product into Escherichia coli DH5a competent cells by the usual method, construct the Escherichia coli strain containing the recombinant cloning vector, and use an agarose plate (amp + ) and blue-white-white screened positive clones, and picked white-white clones f...

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Abstract

The invention discloses an ELISA agent box to test Asial foot-and-mouth disease virus and making method of the agent box, which comprises the following parts: solid support to connect the detected sample, mono-clone antibody to clad Asial foot-and-mouth disease virus on the solid support connected with the detected sample, multi-clone antibody of guinea pig as dianti-Asial foot-and-mouth disease virus, rabbit anti-guinea pig lgG marked by enzyme, standard serum, colorless substrate, terminal liquid.

Description

technical field [0001] The invention relates to a kit for detecting foot-and-mouth disease virus and a preparation method thereof, in particular to an ELISA kit and a preparation method for detecting Asia1 type foot-and-mouth disease virus. Background technique [0002] Foot-and-mouth disease is a major animal disease. All countries in the world attach great importance to the inspection, quarantine, prevention and control of the disease. At present, the main method for detecting and diagnosing FMD is to detect the FMD virus and its antibody level in the diseased or infected animals, such as the method of isolating the virus. Isolating the virus is the most reliable diagnosis of FMD, but a longer cycle will delay the optimal control time of this severe infectious disease; such experimental methods require expensive instruments and professional technicians. Therefore, the development of a fast, convenient, and field-use detection kit for direct detection of FMD virus antigens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/543
Inventor 邵军军林彤常惠芸周广青独军政丛国正李克生杜惠芬谢庆阁
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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