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Method for extracting and detecting polyphenol in bamboo shoots

A detection method and technology for bamboo shoots, which are applied in pharmaceutical formulations, color/spectral property measurement, medical preparations containing active ingredients, etc., can solve the problem of the lack of systematic research reports on the extraction and detection methods of bamboo shoots polyphenols, and the undetectable content and content. Low problems, to achieve the effect of optimizing the detection system and detection conditions, strong antioxidant activity, and simple operation

Active Publication Date: 2014-02-19
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development and research on the health effects of bamboo shoots is still blank; at the same time, we have found in many studies that another important plant health-care substance - flavonoids, has a very low content in bamboo shoots, and the content can hardly be detected by conventional detection methods. Therefore, it is speculated that polyphenols may be the main health-care substances in bamboo shoots
In addition, there is a lack of systematic research reports on the extraction and detection methods of bamboo shoot polyphenols

Method used

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  • Method for extracting and detecting polyphenol in bamboo shoots
  • Method for extracting and detecting polyphenol in bamboo shoots
  • Method for extracting and detecting polyphenol in bamboo shoots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The composition of the polyphenol detection system includes: polyphenol extract 0.25mL, water 1.75mL, Folin-ciocalteu color reagent 1mL, Na 2 CO 3 1mL. Detection conditions include reaction temperature, reaction time, and detection wavelength.

[0037] Optimization of Na2CO3 concentration and reaction temperature in polyphenol detection system:

[0038] The fresh bamboo shoots of Chinese rose and bamboo stalks were collected, chopped and combined, and the polyphenols were extracted with acetone as the solvent as the detection material. The dilution factor of Folin-ciocalteu chromogen is set to 3 times and 4 times, Na 2 CO 3 The concentration was set to 5%, 10% and 15%, and the reaction temperature was set to 20°C, 25°C, 30°C, 35°C. Experiments show that when Na 2 CO 3 When the concentration is 20% and 30%, a precipitate is formed after the water bath reaction, so Na 2 CO 3 The upper limit of the concentration setting is 15%.

[0039] After each reaction sample...

Embodiment 2

[0045] Fresh bamboo shoots of May rose and bamboo stalks were collected, cut into pieces, and polyphenols were extracted with acetone as a solvent for detection. The dilution factor of Folin-ciocalteu chromogenic reagent is set as 1 times, 2 times, 3 times, 4 times, 5 times, 6 times, 7 times. The reaction time is set to 0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h. The detection wavelength is set to 755nm, 760nm, 765nm, 770nm. In the reaction system with 15%Na 2 CO 3 It is an alkaline component, and the reaction temperature is a water bath at 25°C.

[0046] Each reaction combination was reacted at 25°C for a corresponding time, and then the absorbance values ​​at 755nm, 760nm, 765nm, and 770nm were simultaneously measured in an ultraviolet spectrophotometer. The results are shown in Table 2-Table 5.

[0047] Table 2 The detection effect when the dilution factor of Folin-ciocalteu chromogen is 1 times and 2 times

[0048]

[0049] Table 3 The detection effect when the dilution f...

Embodiment 3

[0061] The inventor has studied for many years and through many experiments, and found that under the same conditions, polyphenols in bamboo shoots were extracted with distilled water, various acetone and ethanol of the same concentration, and the light absorption value of the acetone extract in the same detection system was often Higher than distilled water and the extract of the same concentration of ethanol, it shows that the extraction effect of acetone is better than that of distilled water and ethanol. Therefore, it was decided to use acetone as the solvent for extracting polyphenols from bamboo shoots. Next, optimize the appropriate acetone concentration.

[0062] The acetone concentration was set to 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%.

[0063] Collect fresh bamboo shoots of May rose and tea stalk bamboo, freeze them in liquid nitrogen, smash them into pieces, mix the shoots and meat and store them at -70°C. When extracting, take out a certain amount of bamboo ...

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Abstract

The invention discloses a method for extracting and detecting polyphenol in bamboo shoots. The method comprises the following steps: (1) preparing materials, namely, selecting fresh bamboo shoots, freezing in liquid nitrogen, knocking to shell, and preserving the bamboo shoot meat at minus 70 DEG C for later use; (2) selecting acetone as an extraction solvent, and extracting polyphenol in the bamboo shoots through an ultrasonic low-temperature auxiliary solvent; (3) detecting by using a Folin-ciocalteu method, wherein the detection system comprises 0.25ml of polyphenol extracting solution, 1.75ml of water, 1ml of Folin-ciocalteu color developing agent which is diluted for 6 times and 1ml of 5-15% Na2CO3; the detection condition is that the reaction temperature is 25-35 DEG C in water bath, the reaction time is 0.5-3.5 hours, and the detection wavelength is 755-770nm. Due to adoption of the method, a system for detecting the polyphenol in fresh bamboo shoots and a system for extracting the polyphenol in the bamboo shoots are optimized and established, the detection is rapid and convenient, the extraction efficiency is high, and theoretical foundation can be provided for bamboo shoot development and utilization.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for extracting and detecting polyphenols in bamboo shoots. Background technique [0002] Polyphenols are compounds in plants that have health-promoting properties; found in some common plant foods such as cocoa beans, tea, soybeans, red wine, vegetables and fruits. Polyphenols have a strong antioxidant effect. Oxidative damage is an important cause of many chronic diseases, such as cardiovascular disease, cancer and aging. The antioxidant function of polyphenols can play a preventive role in these chronic diseases. For example, polyphenols in chocolate are strong antioxidants, which can inhibit the oxidation of low-density lipoprotein (LDL) cholesterol. In addition to the antioxidant effect of polyphenols, according to the report of FEDERATION of AMERICAN SOCIETIES for EXPERIMENTAL BIOLOGY (FASEB) in January 2008, Israeli researchers discovered for the first time that taking polypheno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899G01N21/31
Inventor 刘长国
Owner ZHEJIANG FORESTRY UNIVERSITY
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