O type foot-and-mouth disease 146S antigen quantitative ELISA detection kit and method for using same

Abstract

The invention discloses an O type foot-and-mouth disease 146S antigen quantitative ELISA (enzyme-linked immuno sorbent assay) detection kit and a method for using the same. The kit comprises an ELISA plate, an O type foot-and-mouth disease standard reference antigen, a demulsifier, an O type foot-and-mouth disease rabbit antiserum, an O type foot-and-mouth disease guinea pig antiserum, a rabbit anti-guinea pig-horse radish peroxidase conjugate, a guinea pig antiserum dilute solution, a 25-fold PBST (phosphate buffer solution tween) concentrated solution, a carbonate buffer solution capsule, a citric acid-phosphate buffer solution tablet, an OPD (o-phenylenediamine) tablet, a stop solution, a plate sealing membrane, a moving liquid tank and a 96-mesh U-shaped dilution plate. The kit is an organic combination of a sucrose density gradient centrifugation method and an indirect sandwich ELISA method, integrates the advantages of the sucrose density gradient centrifugation method and the indirect sandwich ELISA method, is simple to operate and good in stability, is suitable for batch detection, can be used for distinguishing serum types, and is an ideal substitution method for antigen quantitative and vaccine efficacy detection.

Description

technical field [0001] The invention relates to a kit, in particular to a type O foot-and-mouth disease 146S antigen quantitative ELISA detection kit and a use method thereof. Background technique [0002] Foot and mouth disease (FMD) is an acute, febrile and highly contagious infectious disease common to major domestic animals such as pigs, cattle, sheep, and other cloven-hoofed animals, with more than 70 susceptible animals. The clinical feature is a vesicular eruption on the oral mucosa, hooves, and breast skin. The disease has many transmission routes and fast speed, and has repeatedly broken out in the world, causing huge political and economic losses. The World Organization for Animal Health (OIE) lists it as an infectious disease that must be declared, and my country also lists it as the first class of animal infectious diseases. [0003] Foot and mouth disease virus (FMDV) belongs to the genus FMDV of the picornaviridae family, and has seven serotypes: O, A, Asian ...

AI Technical Summary

Problems solved by technology

However, this method also has its disadvantages: complex operation, expensive equipment, poor repeatability, not suitable for the detection of a large number of samples, and cannot distinguish serotypes

According to reports, the detection results of this method are in good agreement with the sucrose density gradient centrifugation method, and the operation is simpler than the latter; but like the latter, it cannot distinguish serotypes

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