O type foot-and-mouth disease 146S antigen quantitative ELISA detection kit and method for using same

A detection kit and technology for foot-and-mouth disease, which are applied in the field of O-type foot-and-mouth disease 146S antigen quantitative ELISA detection kits, can solve the problems of poor repeatability, complicated operation, expensive instruments, etc.
CN103076451AActive Publication Date: 2013-05-01LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2013-05-01

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Abstract

The invention discloses an O type foot-and-mouth disease 146S antigen quantitative ELISA (enzyme-linked immuno sorbent assay) detection kit and a method for using the same. The kit comprises an ELISA plate, an O type foot-and-mouth disease standard reference antigen, a demulsifier, an O type foot-and-mouth disease rabbit antiserum, an O type foot-and-mouth disease guinea pig antiserum, a rabbit anti-guinea pig-horse radish peroxidase conjugate, a guinea pig antiserum dilute solution, a 25-fold PBST (phosphate buffer solution tween) concentrated solution, a carbonate buffer solution capsule, a citric acid-phosphate buffer solution tablet, an OPD (o-phenylenediamine) tablet, a stop solution, a plate sealing membrane, a moving liquid tank and a 96-mesh U-shaped dilution plate. The kit is an organic combination of a sucrose density gradient centrifugation method and an indirect sandwich ELISA method, integrates the advantages of the sucrose density gradient centrifugation method and the indirect sandwich ELISA method, is simple to operate and good in stability, is suitable for batch detection, can be used for distinguishing serum types, and is an ideal substitution method for antigen quantitative and vaccine efficacy detection.
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Description

technical field

[0001] The invention relates to a kit, in particular to a type O foot-and-mouth disease 146S antigen quantitative ELISA detection kit and a use method thereof. Background technique

[0002] Foot and mouth disease (FMD) is an acute, febrile and highly contagious infectious disease common to major domestic animals such as pigs, cattle, sheep, and other cloven-hoofed animals, with more than 70 susceptible animals. The clinical feature is a vesicular eruption on the oral mucosa, hooves, and breast skin. The disease has many transmission routes and fast speed, and has repeatedly broken out in the world, causing huge political and economic losses. The World Organization for Animal Health (OIE) lists it as an infectious disease that must be declared, and my country also lists it as the first class of animal infectious diseases.

[0003] Foot and mouth disease virus (FMDV) belongs to the genus FMDV of the picornaviridae family, and has seven serotypes: O, A, Asian ...

Claims

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