Method for extracting plastocyte of mouse

Abstract

The invention relates to an extraction method of mouse platelet. Blood drawn from orbita vein of mouse is placed into an anticoagulation centrifugal tube, centrifugated at the speed of 800rmp for 10min after standing for 30min, and the extracted supernatant fluid is plasma rich in platelet. The supernatant fluid is placed in a clean test tube and centrifugated at the speed of 3500rpm for 10min. After filtering the supernatant fluid, the sediments at the bottom of the tube are the platelets. 50ul to 100ul of ammonium oxalate solution with a mass concentration of 1 percent is added into the test tube and slightly stirred with a glass rod, and then 2ml to 3ml of ammonium oxalate solution with a mass concentration of 1 percent is added, and stood for 5min, to make sure the hematid is dissolved; the hematid is centrifugated at the speed of 3500rpm for 10min, after the supernatant fluid is abandoned, the test tube is added with a few platelet scrub solution and blown and beaten to turn the platelet into a platelet suspension. The suspension is centrifugated at the speed of 3300rpm for 10min, removed the supernatant in the suspension, the test tube is added with a few platelet scrub solution and blown and beaten to turn the platelet into a platelet suspension. The platelet is used as an antigen to immunify guinea pigs, and the serum of such guines pigs is injected into the mouse to prepare allergic purpura. The preparation method has simple operation, can obtain high concentration platelet, and can comparatively better solve the problem of impure platelet.

Description

Technical field:

[0001] The invention relates to a method for extracting mouse platelets. The extracted platelets are used as antigens to immunize guinea pigs, and the immune serum of the guinea pigs will be used to prepare mouse idiopathic thrombocytopenic purpura animal models. Background technique:

[0002] The pathogenesis of idiopathic thrombocytopenic purpura (ITP) is closely related to immunization, and the ITP model induced by immunization is highly similar to human ITP in terms of pathological characteristics and healing mechanism, so the experimental research mainly uses immunization to establish animal models, small The mouse ITP model is highly sensitive to various anti-ITP drugs, so the successful preparation of the mouse ITP model is one of the key technologies for the research of anti-ITP drugs. However, a significant problem with this modeling method is that the purity of the antigen is not enough, that is, the purity of the platelets isolated from the whole ...