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Polypeptide vaccine of anti Asiatic I virus of foot-and-mouth disease and its preparing method

A technology of foot-and-mouth disease virus and polypeptide vaccine, which is applied in the field of polypeptide vaccine against Asian type-1 foot-and-mouth disease virus and its preparation, which can solve the problems of incomplete inactivation and achieve strong protection, strong immunogenicity and good safety

Inactive Publication Date: 2004-01-28
FUDAN UNIV
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AI Technical Summary

Problems solved by technology

Inactivated vaccines are currently widely used in most countries and regions, but there are also safety problems, mainly due to incomplete inactivation or improper protection during processing, which allows the virus to escape from the processing plant and cause the outbreak and prevalence of FMD

Method used

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  • Polypeptide vaccine of anti Asiatic I virus of foot-and-mouth disease and its preparing method

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Experimental program
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Embodiment Construction

[0012] Select the 133-158 amino acid peptide segment of the Asian-type FMDV VP1 protein B cell epitope and the 20-34 amino acid peptide segment of the VP4 T cell epitope, and combine the two peptide segments into a tandem form of 133-158-20-34-133-158 To enhance the immunogenicity, a total of 13 amino acids were introduced as linkers between the peptides of the above tandem structure. An EcoRI restriction site and a start codon were introduced at the 5' end of the tandem structure, and a stop codon and a BamHI restriction site were introduced at the 3' end. The structure of the tandem gene is shown in the appendix figure 1 .

[0013] The structural characteristics of the tandem gene are as follows: at both ends of the tandem antigen gene, there is a copy of the DNA sequence of VP1133-158 amino acids encoding the B-cell epitope, and a copy of the VP4 amino acid 20-34 encoding the T-cell epitope in the middle. DNA sequences; the two linkers connecting these sequences are "Pro-...

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Abstract

The invention is a kind of polypeptide vaccine and the manufacturing method which can resist Asia one foot and mouth disease virus. The vaccine is prepared by that DNA array of B , T cell form location amino acid on VP1 and VP4 contact with coding form location albumen alone, or link with macromolecule of the carrier coding schedule location merged protein. The invention uses chemical method to synthesize the peptide gene of B cell and T cell form location on coding Asia1 type VP1 and VP4, and they are contacted, the gene is inserted with particle carrier alone or connecting with the carrier molecular, and transferred into bacteria express, and yeasted and gets the product.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a polypeptide vaccine against Asian type I foot-and-mouth disease virus and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is one of the most serious livestock infectious diseases in the world today. It mainly harms occasional animals such as pigs, cattle, and sheep. Huge economic losses, and thus by the International Bureau of Veterinary Medicine class A infectious diseases first. [0003] The causative agent of foot-and-mouth disease is foot-and-mouth disease virus (FMDV). FMDV belongs to the genus Foot-and-Mouth Disease Virus of the family Picornaviridae, and its structure is relatively simple. The complete virion is composed of a single-stranded positive-strand RNA wrapped in a capsid composed of 60 copies of each of the four structural proteins VP1, VP2, VP3, and VP4. is the main antigenic protein. According to diffe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/135A61P31/14
Inventor 郑兆鑫严维耀张青张震宇
Owner FUDAN UNIV
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