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Recombinant bacterium of brucella abortus with immunity labeling and use thereof

A technology of Brucella abortus and recombinant bacteria, which is applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of high cost, threat to human and animal health, and hindrance to the eradication and purification of brucellosis

Inactive Publication Date: 2009-03-18
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, the global strategy for the prevention and control of brucellosis has been mainly quarantine-culling (in developed countries) or immunization (in developing countries). Difficult to operate; the latter can prevent the spread of the disease in the animal population through immunization and auxiliary culling measures, but it is difficult to distinguish the immune animals from the clinically diseased animals, making the diseased animals exist in the natural population for a long time, which seriously threatens human beings and fauna health, impeding the eradication and decontamination of brucellosis in animals

Method used

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  • Recombinant bacterium of brucella abortus with immunity labeling and use thereof
  • Recombinant bacterium of brucella abortus with immunity labeling and use thereof
  • Recombinant bacterium of brucella abortus with immunity labeling and use thereof

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Embodiment 1

[0032] Embodiment 1, the construction of the recombinant bacterium with the Brucella abortus of immune labeling

[0033] According to the perosamine synthetase (perosamine synthetase) sequence (GenBank Accession Number: BAB1-0542) in the Brucella gene, two pairs of primers were designed, one pair of primers was used to amplify the homology arm DA14-1, and the other pair of primers used for the amplification of DA14-2. Homology arm DA14-1 and homology arm DA14-2 are connected to form sequence DA14, and sequence DA14 is inserted into pEX18AP plasmid to obtain recombinant vector pEX18AP-DA14. The recombinant vector pEX18AP-DA14 undergoes homologous recombination with the genome of Brucella abortus to obtain recombinant bacteria of Brucella abortus inactivating the gene encoding perosamine synthetase.

[0034] 1. Construction of recombinant vector pEX18AP-DA14

[0035] For a schematic diagram of the construction process, see Figure 5 .

[0036] 1. Extraction of Brucella genom...

Embodiment 2

[0078] Embodiment 2, the identification of the Brucella recombinant bacterium of inactivating perosamine synthetase (perosamine synthetase)

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Abstract

The invention discloses a recombinant strain for Brucella abortus provided with an immune label. The recombinant strain for the Brucella melitensis provided with the immune label is a strain obtained by killing encoding genes of perosamine synthetase in the Brucella abortus. Compared with an original strain, the obtained strain has lower virulence, and can be used as a vaccine to immunize animals without killing the genes. By utilization of the strain to immunize a mouse, immunoreaction states different from those of the standard strain and the prior vaccine strain can be generated in the body of the mouse, and sicken animals can be identified from immune animal groups by immunological detection of serum of the animals. The modification of the brucelliasis vaccine and the research of a diagnosis and identification agent have important significance in developing a brucelliasis gene deletion marker vaccine and a matched diagnosis and identification agent and promoting the elimination and purification of brucelliasis of the animals all over the world.

Description

technical field [0001] The invention relates to a recombinant bacterium of Brucella abortus with immune marker and application thereof. Background technique [0002] Brucellosis is a zoonotic infectious disease, second only to foot-and-mouth disease among large animal infectious diseases, and has important political and economic influences. The epidemic situation in countries around the world is very serious. For a long time, the global strategy for the prevention and control of brucellosis has been mainly quarantine-culling (in developed countries) or immunization (in developing countries). Difficult to operate; the latter can prevent the spread of the disease in the animal population through immunization and auxiliary culling measures, but it is difficult to distinguish the immune animals from the clinically diseased animals, making the diseased animals exist in the natural population for a long time, which seriously threatens human beings and the health of the fauna, hin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21A61K39/10A61P31/04C12R1/01
Inventor 吴清民牛建蕊任婕赵茜王晓夏王真张春燕李娜刘文娟杨羽刘文晓
Owner CHINA AGRI UNIV
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