Recombinant bacterium of brucella abortus with immunity labeling and use thereof
A technology of Brucella abortus and recombinant bacteria, which is applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of high cost, threat to human and animal health, and hindrance to the eradication and purification of brucellosis
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Embodiment 1
[0032] Embodiment 1, the construction of the recombinant bacterium with the Brucella abortus of immune labeling
[0033] According to the perosamine synthetase (perosamine synthetase) sequence (GenBank Accession Number: BAB1-0542) in the Brucella gene, two pairs of primers were designed, one pair of primers was used to amplify the homology arm DA14-1, and the other pair of primers used for the amplification of DA14-2. Homology arm DA14-1 and homology arm DA14-2 are connected to form sequence DA14, and sequence DA14 is inserted into pEX18AP plasmid to obtain recombinant vector pEX18AP-DA14. The recombinant vector pEX18AP-DA14 undergoes homologous recombination with the genome of Brucella abortus to obtain recombinant bacteria of Brucella abortus inactivating the gene encoding perosamine synthetase.
[0034] 1. Construction of recombinant vector pEX18AP-DA14
[0035] For a schematic diagram of the construction process, see Figure 5 .
[0036] 1. Extraction of Brucella genom...
Embodiment 2
[0078] Embodiment 2, the identification of the Brucella recombinant bacterium of inactivating perosamine synthetase (perosamine synthetase)
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