52results about How to "Strong throughput" patented technology

The invention discloses a Hive platform oriented user safety management system. The Hive platform oriented user safety management system comprises an access control and safety audition unit, a data query unit and an identify authentication unit, wherein the access control and safety audition unit comprises a safety audition server and an access control server, the data query unit comprises an HDFS server, a NameNode server and a Hive server, and the identify authentication unit comprises an LDAP server. A Hive platform oriented user safety management method comprises four steps, including user registration, user login, user query and audition storage. According to the Hive platform oriented user safety management system and method, an exceed authority access action of the traditional user to a large-scale storage system is prevented, data leakage due to the fact that a data storage device is stolen can be safely and effectively prevented, and problems that data packets are captured illegally and private data is obtained after the traditional storage system is invaded by external personnel are prevented.

The invention provides a non-key signature infrastructure building method based on a block chain. The method comprises the steps that a core layer node is used to embed a root hash value of an aggregation layer into an OP_RETURN transaction script through identity authentication; broadcasting is carried out in the block chain network; packaging into the block is carried out to form a KSI Calendar hash account book; KSI release layer architecture based on the block chain is provided; a miner plays the role of release node in KSI; transaction information in the block chain network is collected; a Merkle tree is built; the release node simply needs to extract contents in OP_RETURN in transaction for Merkle tree building, so as to finally form CRH; and finally CRH is written into the block head. According to the invention, without changing the current non-key signature infrastructure architecture, the block chain is used to complete identity authentication of the KSI core layer node; the public transparency of the Calendar hash account book is protected; and the problems of long CRH release cycle, poor release media credibility and difficult recognition and processing in the prior art are solved.

The invention discloses a preparation method of an anti-pollution polyamide film composite film. The preparation method comprises following steps: a water-soluble divalent metal salt and a water phasemonomer are added into water respectively so as to obtain a water phase monomer solution containing divalent metal ions; a porous support layer surface is immersed in the water phase monomer solutioncontaining divalent metal ions, is taken out from the water phase monomer solution containing divalent metal ions, and is immersed in an organic phase monomer solution for immersion; and at last, slaking treatment is carried out so as to obtain the anti-pollution polyamide film composite film. The preparation method possesses following advantages: the pertinence is high, cost is low, the obtainedproduct is nontoxic, the technology is simple, operation is convenient, and the preparation method is convenient for large scale industrialized production.

The invention discloses a CYP2C19 gene polymorphyism detection kit and a detection method thereof. The kit comprises ultrapure water, a solution X, a 10*PCR (polymerase chain reaction) buffer solution, PCR primers, a 25mM magnesium chloride solution, DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the PCR primers include a different genotypic forward and reverse amplification primer, an internal DNA reference forward and reverse amplification primer and an internal reaction reference forward and reverse amplification primer on three SNP (single nucleotide polymorphism) sites of a CYP2C19 gene; and gene sequences of the primers are shown as SEQ ID NO. 1-NO. 13 (sequence identifier number 1-number 13). The detection method comprises the steps of collecting a sample, extracting nucleic acid, taking nucleic acid of a patient as a template to conduct PCR reaction, and using a GeXP genetic analyzer to conduct capillary electrophoretic separation on the sample. The detection kit and the detection method have the advantages of high specificity, high sensitivity, high flux, high reliability, low cost and no false negative results.

The invention discloses a kit for detecting helicobacter pylori drug resistance gene polymorphism by a multiplex fluorescence PCR melting curve method. The helicobacter pylori drug resistance gene comprises the following three genes: a 23S rRNA gene, a 16S rRNA gene and a gyr A gene, the kit comprises a nucleic acid extraction reagent and a nucleic acid amplification reagent, the nucleic acid extraction reagent comprises superparamagnetic silicon oxide nano magnetic beads, a lysis solution, a washing solution and an eluent; the nucleic acid amplification reagent comprises a primer pair and a probe which respectively correspond to the helicobacter pylori drug resistance gene 23S rRNA gene, the helicobacter pylori 16S rRNA gene, the gyr A gene and an internal standard gene human housekeepinggene beta-globin. According to the invention, three drug-resistant sites and one internal standard gene can be simultaneously detected in a single tube, so that the detection flux of a sample is improved; meanwhile, interference between multiple pairs of primer probes in a detection system is improved, and the sensitivity and specificity of reagent detection are effectively improved.

The invention discloses a primer composition for guiding nitroglycerin medication and healthy drinking, a multiple gene detection kit and a use method of the kit, and the kit comprises the primer composition, a PCR buffer solution and a positive reference substance, and the PCR buffer solution comprises ultrapure water, an X solution, a 10*PCR (polymerase chain reaction) buffer solution, a PCR primer, a 25mM magnesium chloride solution and DNA polymerase, the primer composition comprise two forward and reverse amplification primers of different gene types on the 2 SNP sites of genes related to the nitroglycerin medication and healthy drinking and forward and reverse amplification primers capable of reflecting internal reference, the gene sequences of the primers are represented as SEQ ID NO.1-NO.8; the use method comprises the step of acquiring a sample and extracting nucleic acid, the step of performing the PCR reaction by using extracted nucleic acid as a template, and the final step of separating the sample through capillary electrophoresis. The primer composition has the advantages of being strong in specificity, high in accuracy, high in flux, strong in reliability, low in cost and free from false negative result.

The invention relates to the technical field of security detection of agricultural and sideline products, in particular to a primer for simultaneously detecting five food-borne pathogenic bacteria through a liquid chip and a detection method. The detection method comprises steps as follows: a specific primer and a gene probe for five foodborne pathogenic bacteria are designed; DNA templates of the detected five foodborne pathogenic bacteria are prepared; PCR (polymerase chain reaction) amplification is performed on the five foodborne pathogenic bacteria; coupling is performed on the gene probe for the five foodborne pathogenic bacteria and microspheres, and a microsphere probe is obtained and verified; an obtained PCR amplification product and the microsphere probe have a hybridization reaction; a fluorescence signal of a hybridized product is detected, and quantitative analysis is performed on the five foodborne pathogenic bacteria. A method capable of simultaneously detecting five common foodborne pathogenic bacteria is successfully established, the variable coefficient is within 2%, the specificity is 100%, the sensitivity can reach 100 CFU/mL, and the method is equivalent to a fluorescent PCR technology and can be applied to high-throughput rapid detection of clinic and environment specimens of foodborne diseases.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for a sterile gene pms3 (photoperiod-sensitive male sterility 3) of paddy rice. The molecular marker is K-120522, wherein the polymorphism of the K-120522 is C or G. By utilizing the molecular marker, the gene pms3 can be quickly and precisely detected; the selection efficiency, in the breeding of the paddy rice, of the gene is greatly improved. The enzyme digestion, the electrophoresis, the sequencing and the like are not needed in a detection process; the operation is simple and convenient; the commercialized high-throughput quick detection can be realized; the aerosol pollution of a PCR (Polymerase Chain Reaction) product, the pollution of EB (Ethidium Bromide) to an environment and the harm of formaldehydeto a human body are thoroughly avoided.

The invention provides a preparation method of a composite film. By the preparation method, an MOF material is combined with an organosilicone precursor material to form an MOF-organosilicone composite film on the surface of a carrier through high-temperature roasting. The composite film is continuous and compact, integrates the advantages of high throughput and a strong carrier binding force of organosilicone and the advantage of strong molecular sieving capacity of the MOF material, solves the problem of a weak bonding force between the composite film prepared based on MOF material and the carrier, and shows relatively high molecular sieving capacity and H2 permeation flux, and has a relatively good application prospect in the field of gas separation, gas purification and the like.

The invention discloses an extraction method of trace metal elements in coke and an application. The extraction method comprises the following steps: taking coke to prepare a sample, uniformly stirring the sample, weighing the sample, putting the sample into a beaker, adding deionized water, performing uniform stirring, and standing for a plurality of hours; filtering the soaked coke soak solutionto ensure that no visible particulate matters exist in the filtrate; adding the filtrate into a volumetric flask, fixing the volume, adding a nitric acid solution, and performing uniform mixing to obtain a first to-be-detected solution; and drying the filter residues, performing ashing, carrying out mixed acid digestion treatment to obtain a second to-be-detected solution; and finally, respectively measuring the content of the target metal element by adopting an EDTA method and an inductively coupled plasma atomic emission spectrometry. The method can extract the metal elements in the coke, has the advantages of simple steps, short time consumption and high extraction efficiency, and can eliminate experiment interference by adopting deionized water.

A process for extracting useful material from the rejected ore powder by high-intensity gradient magentic field includes such steps as creating a strong magnetic field (at least 6X10 to the power 5 A/m), arranging a secondary magnetic pole unit in said magnetic field, making the rejected ore powder flow through said magnetic field, and recovering said useful material from the secondary magnetic pole unit.

The invention relates to a method for detecting the residual iron content in a degreasing agent. In the prior art, the residual iron content in the used degreasing agent cannot be accurately detected.A purpose of the present invention is mainly to solve the technical problem in the prior art. The technical scheme is that the method comprises: preparing a to-be-detected solution, wherein a certainamount of a to-be-detected degreasing agent sample solution is sucked and placed into a conical flask, a certain proportion of a hydrochloric acid solution is injected into the conical flask, the to-be-detected degreasing agent sample solution is subjected to acidification treatment, the acidified to-be-detected degreasing agent sample solution is transferred into a 100 ml volumetric flask, volume metering is performed to achieve a certain scale, and uniform shaking is performed to obtain the to-be-detected solution; determining the spectral intensity of iron in the to-be-detected solution byusing an inductively coupled plasma emission spectrometer; and calculating the residual iron content in the to-be-detected degreasing agent sample solution. According to the present invention, the method has advantages of high detection data accuracy, simple operation and low labor intensity, and can perform the detection and judgment on the actual cleaning effect of the cold rolling degreasing agent used in the production line.

The invention discloses a vehicle speed monitoring method based on a flink architecture, and the method comprises the steps of collecting data through a radar tachymeter, carrying out the data cleaning through a Flink platform, analyzing the data that the vehicle speed is greater than a speed limiting threshold value and the distance between vehicles is less than 200 meters through the flink platform, and obtaining the vehicle driving information; performing message consumption by using the flink platform, feeding overspeed vehicle information in the driving safety range back to the vehicle-mounted system, and enabling the vehicle-mounted system of the vehicle to report the overspeed vehicle condition to a driver; and meanwhile, performing data storage, so that historical data can be read,an overspeed accident investigation basis can be provided for traffic polices, and overspeed accidents can be simulated.

The invention relates to a solid flux culture device and application thereof, and belongs to the technical field of solid culture fermentation. The solid flux culture device comprises a culture plate and a plate cover arranged on the culture plate, wherein the culture plate comprises a culture plate body; a plurality of culture holes are formed in the culture plate body; multilayer gauze matched with the dimension of the culture hole is arranged at the inner bottom of each culture hole; a first through hole is formed in the middle of the bottom of each culture hole; a Luer joint matched with the first through hole is inserted in the first through hole in a penetrating way; a connector of the Luer joint is positioned at the outer part of the culture plate; a plurality of second through holes are formed in the plate cover; the distribution position of the second through hole is consistent with that of the culture holes. The solid flux culture device uses a porous culture plate; the culture flux can be realized on the solid culture medium; the flux screening culture is performed on products with advantages; the obvious directivity is realized.

The invention discloses an organic-inorganic composite film and a preparation method thereof. The organic-inorganic composite membrane is prepared by the following steps: (1) preparing a film formingliquid from an organic film-forming polymer, a polar copolymer and a solvent, (2) transferring the film forming liquid into a film forming coagulation bath to obtain an organic ultrafiltration film, and (3) loading the surface and interior of the organic ultrafiltration film with inorganic particles through an alternate impregnation method to obtain the organic-inorganic composite separation film.The composite film has the characteristics of high hydrophilicity, high flux and high rejection rate of the dye. The preparation method has simple and easy processes and is suitable for continuous and stable production.

The invention discloses a high-throughput quantitative detection kit for food-borne pathogenic bacteria. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.36, of 9 kinds of food-borne pathogenic bacteria; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilledwater. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.

The invention provides primers and a method for detecting Chediak-Higashi syndrome LYST gene mutation based on a high-throughput sequencing technology, wherein the used primer composition comprises the primers for specifically amplifying a LYST gene and specifically amplifying the closely linked SNP sites within a 2 Mb range from the upstream to the downstream of the LYST gene. According to the present invention, the method has advantages of versatility, multi-site SNP sequencing, high throughput, low cost, high sensitivity and strong specificity, can perform embryo pre-transplantation detection on all Chediak-Higashi syndrome gene mutation, and can achieve the rapid detection process and the accurate detection result.

The invention discloses a super all-weather automatic diagnosis and treatment system, and belongs to the field of high and new technology medical treatment. According to the system, the contemporary mature and under mature high and new computer technology and all the links including medical field reception, diagnosis, treatment and nursing are combined with the personal lifetime information of thecitizens to be implemented to design a large system so as to integrate into a truly ultra-modern hospital. All-weather, automatic and standardized diagnosis and treatment of all the diseases incorporated into the system can be realized. With application of the system, the old medical mode can be completely broken through and the medical field can enter a brand-new era. The throughput is high, thepracticability is high, the cure rate is high, the accident rate is low, the hospital management can be strengthened, the medical procedures can be standardized, the human error accident can be reduced, the medical quality can be enhanced, the corruption can be eliminated, the situation of rushing about see a doctor can be omitted for the patient, the working efficiency can be enhanced, the medical environment can be purified, the medical cost can be reduced, the burden of the country can be reduced, the major social problem of medical difficulties can be truly solved and thus the system hasgreat social and economic significance.

The invention provides a primer probe combination for detecting bovine respiratory viruses, a kit and application. According to the primer probe combination for detecting the bovine respiratory viruses, the kit and the application provided by the invention, four main bovine respiratory viruses (BHV-1, BRSV, BPIV-3 and BVDV) can be rapidly, accurately and simultaneously identified on the basis of multiple fluorescent PCR; the detection sensitivity is high; the specificity is high; and the flux is high. The kit for detecting the bovine respiratory virus based on the multiple fluorescent PCR technology can be used for detecting pathogen nucleic acid molecules with high sensitivity and specificity. Compared with a common PCR detection method, the detection method of the kit is more sensitive and specific; and nucleic acid pollution in detection is reduced.

The invention discloses a high-throughput quantitative detection kit for pathogenic vibrio. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.32, of 8 kinds of pathogenic vibrio; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilled water. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.

The invention discloses a high-throughput quantitative detection kit for diarrhea pathogenic bacteria. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.36, of 9 kinds of diarrhea pathogenic bacteria; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilled water. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.