Ultrasonic extraction atomization auxiliary carbon fiber ionization device and method of using same to realize ionization

An atomization device and ionization technology, applied in the field of mass spectrometry, can solve the problems of low ionization efficiency of polypeptide compounds, inability to perform rapid and direct in-situ analysis, poor accuracy of quantitative analysis, etc., achieving significant progress and practical value , Strong practicability and convenient sampling effect

Pending Publication Date: 2019-11-01
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the ionization efficiency of the carbon fiber ion source used in the existing carbon fiber ion mass spectrometry is not high or even impossible for the ionization of polypeptide compounds, so that the carbon fiber ion mass spectrometry cannot realize the specific analysis of polypeptide compounds. In addition, the current carbon fiber When ion mass spectrometry is used for quantitative analysis of complex samples, the matrix effect is high, which will lead to poor accuracy of quantitative analysis. Therefore, the sample needs to be pre-treated, and rapid and direct in-situ analysis cannot be achieved.

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  • Ultrasonic extraction atomization auxiliary carbon fiber ionization device and method of using same to realize ionization
  • Ultrasonic extraction atomization auxiliary carbon fiber ionization device and method of using same to realize ionization
  • Ultrasonic extraction atomization auxiliary carbon fiber ionization device and method of using same to realize ionization

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Embodiment 1

[0071] Using the ultrasonic extraction and atomization-assisted carbon fiber ionization device described in the present invention and a mass spectrometer (mass analyzer is a triple quadrupole) to carry out mass spectrometry analysis of polypeptides (glutathione) and macromolecular compounds (angiotensin II):

[0072] Dissolve solid glutathione and angiotensin II in the extraction solvent methanol solution respectively, and prepare sample solutions with a concentration of 10 μg / mL for use; introduce the auxiliary solvent methanol through the metal hollow tube 6 (flow rate 5 μL / min), Turn on the high-voltage power supply 3, and apply a high voltage of +3000V to the carbon fiber 4; respectively take 3 μL of 10 μg / mL sample solution, and drop them on the ultrasonic extraction and atomization device 2 (ultrasonic atomization sheet), and the ultrasonic extraction and atomization device 2 is The sample solution is first extracted and then atomized. The atomized sample is collected and...

Embodiment 2

[0075] Adopt ultrasonic extraction atomization auxiliary carbon fiber ionization device described in the present invention and mass spectrometer (mass analyzer is a triple quadrupole) to the actual sample without any pretreatment: Chinese medicine cinnamon slices, Chinese medicine Angelica dahurica powder and compound sulfamethoxine azole tablets for mass spectrometry analysis:

[0076] The traditional Chinese medicine cinnamon slices, traditional Chinese medicine Angelica dahurica powder and compound sulfamethoxazole tablets purchased from the market are used as spare samples; the auxiliary solvent methanol (flow rate 5 μL / min) is introduced through the metal hollow tube 6, and the high-voltage power supply 3 is connected. 4 Apply a high voltage of +3000V; directly place the traditional Chinese medicine cinnamon tablets, traditional Chinese medicine Angelica dahurica powder and compound sulfamethoxazole tablets without any pretreatment on the ultrasonic extraction atomization ...

Embodiment 3

[0079] Adopt ultrasonic extraction and atomization auxiliary carbon fiber ionization device described in the present invention and mass spectrometer (mass analyzer is a triple quadrupole) to use ESI poor solvent n-hexane and sherwood oil to carry out mass spectrometry analysis to traditional Chinese medicine Cortex Cortex Phellodendri and Salvia Miltiorrhiza:

[0080] The Chinese medicine Phellodendron Phellodendron and Chinese medicine Salvia miltiorrhiza purchased from the market are used as spare samples; the auxiliary solvent n-hexane or petroleum ether (flow rate 5 μL / min) is introduced through the metal hollow tube 6, the high-voltage power supply 3 is connected, and +3000V is applied to the carbon fiber 4. High voltage; respectively place Chinese medicine Cortex Cortex Phellodendri and Chinese medicine Salvia Miltiorrhiza directly on the ultrasonic extraction atomization device 2 (ultrasonic atomization sheet), and then add an appropriate amount of ESI poor solvent n-hexa...

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Abstract

The invention discloses an ultrasonic extraction atomization auxiliary carbon fiber ionization device and a method of using the same to realize ionization. The device includes a carbon fiber ion source and a mass spectrometry sample input channel. A sampling and ionization end of the carbon fiber ion source is located in front of a port of the mass spectrometry sample input channel. An ultrasonicextraction atomization device is arranged near the sampling and ionization end of the carbon fiber ion source. The method of using the ultrasonic extraction atomization auxiliary carbon fiber ionization device of the invention to realize ionization includes the operations of: placing a sample, which contains an extraction solvent, on the ultrasonic extraction atomization device, then sequentiallycarrying out extraction and atomization on the sample by the ultrasonic extraction atomization device, and then ionizing the atomized sample by the carbon fiber ion source. The ultrasonic extraction atomization auxiliary carbon fiber ionization device provided by the invention has good an ionization effect on peptide compounds, and can qualitatively and quantitatively analyze complex samples without the need for preprocessing the to-be-tested samples.

Description

technical field [0001] The invention relates to an ultrasonic extraction atomization-assisted carbon fiber ionization device and a method for realizing ionization using the device, belonging to the technical field of mass spectrometry. Background technique [0002] Mass spectrometry (MS) is characterized by fast analysis speed, strong specificity and high sensitivity. It is a powerful tool for analyzing complex mixtures, providing information about molecules, weight and chemical structure of analytes. In recent years, with the continuous improvement and innovation of related technologies, mass spectrometry has become one of the fastest-growing analytical techniques, and it has also played a decisive role in promoting the rapid development of other disciplines, especially life sciences, and opened up many emerging or interdisciplinary fields. At present, mass spectrometry is more and more widely used in the fields of chemistry and chemical engineering, biology and life scien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/28
CPCG01N27/62G01N1/28
Inventor 郭寅龙王政永曹宇奇陆颖洁朱苏珍张立张菁
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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