Solid flux culture device and application thereof

A culture device and flux technology, which is applied in the directions of biochemical cleaning devices, solid-phase fermentation bioreactors, enzymology/microbiology devices, etc. , The effect of powerful flux and high coincidence

Active Publication Date: 2017-12-08
FOSHAN HAITIAN FLAVOURING & FOOD CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A solid fermentation culture device and method (201510012258.8), a microbial solid-state seed production and fermentation device (201310466186.5), a method for microbial solid-state culture using a microbial solid-state culture device (201310717824.6), a solid-state nutrient matrix suspension The method for cultivating filamentous fungi (00103542.8) is a relatively large-scale solid-state culture device. These devices can realize solid-state culture or suspension culture of filamentous fungi with large volume and capacity, but cannot realize the flux of solid-state culture and detection.

Method used

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  • Solid flux culture device and application thereof
  • Solid flux culture device and application thereof
  • Solid flux culture device and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Such as figure 1 , figure 2 and image 3 As shown, a solid-state flux culture device according to an embodiment of the present invention includes a culture plate 24 and a plate cover 27 arranged on the culture plate 24; the culture plate 24 includes a culture plate body 25, and the culture plate body 25 is provided with multiple Culture hole 28, the inner bottom of each culture hole 28 is all provided with the multilayer gauze that matches with culture hole 28 sizes; A luer connector 29 matched with a through hole, the interface 26 of the luer connector 29 is located outside the culture plate body 25; the plate cover 27 is provided with a plurality of second through holes 30, and the distribution positions of the second through holes 30 are all in line with the Culture hole 28 is identical.

[0040] The solid-state flux culture device of the present invention adopts a porous culture plate 24, which can realize the flux of culture on the solid-state medium, and condu...

Embodiment 2

[0057] In this embodiment, the solid-state flux culture device of the present invention is used to ferment the koji material used in the fermentation of Aspergillus oryzae soy sauce.

[0058] (1) Experimental device

[0059] The structure of the solid-state flux culture device used in this example is the same as in Example 1, and the specifications of the components in the solid-state flux culture device are as follows:

[0060] Air delivery unit: The air delivery unit is a commercially available oxygen pump, which can adjust the size of the air flow, and the maximum flow can reach 5m 3 / h, with 4 air outlets at the same time, the ventilation flow control of this test is: the ventilation volume is 0.3m before 11 hours 3 / h, the ventilation volume is 0.8m between 11 hours and 17 hours 3 / h, the aeration volume is controlled at 1m from 17 hours to the end of fermentation 3 / h.

[0061] Air filter: It is a commercially available PP plastic air filter. The pore diameter of th...

Embodiment 3

[0079] In this example, the inventive solid-state flux culture device was applied to the screening of high-production protease strains of Aspergillus oryzae soy sauce fermentation. In this embodiment, Aspergillus oryzae Huyao 3.042 is used as the starting bacterium, and the spore suspension is subjected to mutagenesis treatment (the spore lethality rate is 80%) by using ARTP, and the spores treated with mutagenesis are diluted and applied to the cheese according to a certain ratio. Protein plate medium (medium formula: casein 1%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, disodium hydrogen phosphate 0.1%, agar powder 1.5%, pH 6.7.) for culture and screening. Select the colony with good colony growth, vigorous mycelium growth and large diameter of transparent circle on the medium plate as the target colony, transfer to slant culture, and store the strain after maturity. Pick 24 bacteriums with stable shape (comprising Aspergillus oryzae Huyao 3.042 as the co...

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Abstract

The invention relates to a solid flux culture device and application thereof, and belongs to the technical field of solid culture fermentation. The solid flux culture device comprises a culture plate and a plate cover arranged on the culture plate, wherein the culture plate comprises a culture plate body; a plurality of culture holes are formed in the culture plate body; multilayer gauze matched with the dimension of the culture hole is arranged at the inner bottom of each culture hole; a first through hole is formed in the middle of the bottom of each culture hole; a Luer joint matched with the first through hole is inserted in the first through hole in a penetrating way; a connector of the Luer joint is positioned at the outer part of the culture plate; a plurality of second through holes are formed in the plate cover; the distribution position of the second through hole is consistent with that of the culture holes. The solid flux culture device uses a porous culture plate; the culture flux can be realized on the solid culture medium; the flux screening culture is performed on products with advantages; the obvious directivity is realized.

Description

technical field [0001] The invention relates to a solid-state flux culture device and its application, in particular to a solid-state flux culture device suitable for filamentous fungi and its application, and belongs to the technical field of solid-state culture and fermentation. Background technique [0002] Filamentous fungi are aerobic microorganisms that are widely used in the production of fermented foods, enzymes, pigments, antibiotics, organic acids and flavor compounds due to their ability to secrete abundant extracellular proteins and secondary metabolites. For the utilization of the products of filamentous fungi, solid-state fermentation and liquid submerged fermentation technologies are mainly used. Solid-state fermentation is a kind of fermentation close to the natural growth state of filamentous fungi, which can tolerate high concentrations of nutrient salts, grow rapidly in substrates with low water content, have a low probability of bacterial contamination, a...

Claims

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Application Information

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IPC IPC(8): C12M1/16C12M1/04C12N1/14C12R1/645
CPCC12M21/16C12M25/06C12M29/04C12M29/06C12N1/14
Inventor 周斌王静
Owner FOSHAN HAITIAN FLAVOURING & FOOD CO LTD
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