Primer and probe for RNA-isothermal-amplification detection on brucella abortus, kit and detection method

A constant temperature amplification detection technology for Brucella, applied in the direction of microorganism-based methods, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem of high requirements for culture conditions and the inability to monitor the concentration of Brucella and bacteria in time low level problem

Pending Publication Date: 2020-09-11
张家口富熙生物科技有限公司
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Problems solved by technology

[0004] Due to the low concentration of bacteria and high requirements for culture conditions, the traditional detection method of Brucella

Method used

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  • Primer and probe for RNA-isothermal-amplification detection on brucella abortus, kit and detection method
  • Primer and probe for RNA-isothermal-amplification detection on brucella abortus, kit and detection method
  • Primer and probe for RNA-isothermal-amplification detection on brucella abortus, kit and detection method

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[0149] According to some embodiments of the present invention, the probe sequence comprises: CCGGAUCCAACAAAAGAAAGAAAUCGCG, or at least 10 nucleotides in a homologous sequence thereof.

[0150] According to some embodiments of the present invention, the homologous sequence refers to a nucleotide sequence that is at least 70% identical to the sequence.

[0151] According to some embodiments of the present invention, the homologous sequence has at least 70% identical nucleotide sequence, for example at least 75%, at least 80%, at least 85%, at least 88%, at least 90%, at least 93%, A nucleotide sequence that is at least 95%, at least 97%, at least 98%, or at least 99% identical.

[0152] According to some embodiments of the present invention, the homologous sequence has substantially the same activity as the sequence disclosed in the present invention.

[0153] According to some embodiments of the present invention, the probe sequence comprises at least 10 nucleotide sequences, for examp...

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Abstract

The invention discloses a primer-probe for RNA-isothermal-amplification detection on brucella abortus, a kit and a detection method. The specific detection primer-probe, the detection kit containing the detection primer-probe and the detection method employing RNA isothermal amplification by utilizing the detection kit have the characteristics of high sensitivity, high specificity, low pollution (an amplification product RNA is easy to degrade in a natural environment) and rapidness, have low requirements on instruments and are simple in operation process.

Description

Technical field [0001] The invention relates to the technical field of detection of pathogenic bacteria and microorganisms, in particular to a rapid detection technology for detecting Brucella based on RNA constant temperature amplification, and in particular to a specific detection primer probe, kit and detection method for detecting Brucella. Background technique [0002] Brucella is a gram-negative aerobic bacterium. It is classified as Brucella. Bacteria of this genus are non-acid resistant, without spores, without capsules, without flagella, and have a ball-shaped rod shape. Brucella infects livestock first. The clinical manifestations of livestock are not obvious. However, pregnant female animals are very easy to cause miscarriage or stillbirth. The amniotic fluid, placenta, and secretions that are discharged contain a large amount of Brucella, which is particularly infectious. The bacteria can be found in its fur, urine, feces, and milk. Bacteria excretion can last fo...

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6844C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689C12Q1/6844
Inventor 刘喜富乌日琴郑思晴
Owner 张家口富熙生物科技有限公司
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