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Mass spectrometric detection method for vitamin D in peripheral trace blood

A mass spectrometry detection and vitamin technology, which is applied in the field of peripheral trace blood vitamin D mass spectrometry detection, can solve the problem of inability to detect 25-hydroxyvitamin D and the like

Inactive Publication Date: 2020-11-24
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0005] However, due to the influence of the depth of the blood collection site, interstitial fluid interference and other factors, the micro blood collection is different from the normal blood test results. At present, it is not possible to use the micro blood method to detect 25-hydroxyvitamin D

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  • Mass spectrometric detection method for vitamin D in peripheral trace blood
  • Mass spectrometric detection method for vitamin D in peripheral trace blood
  • Mass spectrometric detection method for vitamin D in peripheral trace blood

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[0036] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0037] The terms "first", "second", "third", "fourth" and the like in the description and claims of the present invention are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence .

[0038] The purpose of the present invention is to provide a mass spectrometry detection method for vitamin D in peripheral trace blood, which is based on the traditional determination of 25(OH)D in venous constant serum by HPLC-MS / MS, and establishes an HPLC-MS / MS method for determination A new method for 25(OH)D in finger trace blood; simple, fast, high sensitivity, less interference, good accuracy and reproducibility; provides a reliable...

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Abstract

The invention relates to a mass spectrometric detection method for vitamin D in peripheral trace blood. The method comprises the steps of serum specimen collection, preparing a detection material anda chemical product; calibration standard, venous blood cleaner, a trace serum 25-hydroxyvitamin D detection method, analyzing data, evaluating the analysis performance of an HPLC-MS / MS method, verifying the result of a vein and trace blood HPLC-MS / MS analysis method, comparing the level of 25-hydroxyvitamin D in vein and trace blood, and comparing the concentration result of 25-hydroxyvitamin D invein and trace blood specimens. The method has the advantages that trace blood and constant blood of the same object are collected at the same time, the 25-hydroxyvitamin D levels of the constant blood and the trace blood are detected at the same time through the high performance liquid chromatography-tandem mass spectrometry, the correlation and conversion coefficient of 25-hydroxyvitamin D of the constant blood and the trace blood are analyzed through the linear regression analysis method, and therefore a conversion formula is obtained; the concentrations of 25-hydroxyvitamin D in constantblood and trace blood can be mutually converted through a formula; and a reliable basis is provided for clinical VitD detection by replacing constant blood with trace blood.

Description

technical field [0001] The invention relates to the technical field of vitamin D detection in peripheral trace blood, in particular to a mass spectrometry detection method for vitamin D in peripheral trace blood. Background technique [0002] Vitamin D is a fat-soluble vitamin that functions as a steroid hormone. Vitamin D (VitD) deficiency is a major public health problem facing children's health. [0003] Studies have shown that: 6-12 months old infants VitD deficiency deficiency rate as high as 35% -40%. The diagnostic criteria for vitamin D deficiency are based on the concentration of 25-hydroxyvitamin D in serum. At present, liquid chromatography-tandem mass spectrometry is internationally recognized as the most reliable method for detecting 25-hydroxyvitamin D, which has high sensitivity, specificity and accuracy, and can simultaneously measure serum 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 concentration. [0004] Clinical determination of serum VitD usually r...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7233G01N30/8675
Inventor 余晓丹焦先婷
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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