Method for jointly detecting sFlt-1/PLGF and HLA-G for detecting pre-eclampsia

Abstract

The invention relates to a method for jointly detecting sFlt-1 / PLGF and HLA-G for predicting pre-eclampsia. The method comprises the following steps: collecting serum specimen: respectively collecting5-10 ml of venous blood from pregnant women in a pre-eclampsia group and normal pregnant women with same gestational weeks, centrifuging all specimens in a low temperature centrifuge at 3000 rpm for10 min within 4 h, taking the serum and subpackaging in EP tubes, and storing in a refrigerator at minus 80 DEG C for testing; coating: using a 0.05 M PH9.0 carbonate coated buffer solution to diluteantibodies to the protein content of 1-10[mu]g / ml, adding 0.1ml of the diluted antibodies into reaction holes of each polystyrene board, staying overnight at 4 DEG C, discarding the solution in the holes the next day, and washing for 3 times; setting up standard holes and sample holes, washing, and setting up blank holes, negative control holes and positive control holes at the same time; adding enzyme-labeled antibodies; adding 100 [mu]L of horseradish peroxidase-labeled detection antibodies into each of the standard holes and the sample holes except for the blank holes, sealing the reactionholes with microplate sealers, and incubating in a water bath kettle or an incubator at 37 DEG C for 60 min; performing board-washing; adding 50 [mu]L of a substrate A and a substrate B into each of the holes, and measuring OD values of each hole. The method is high in detection specificity and sensitivity.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a method for combined detection of sFlt-1 / PLGF and HLA-G to detect preeclampsia. Background technique [0002] Pre-eclampsia is a syndrome of hypertension, edema, and proteinuria that occurs in 7-15% of all pregnancies and is the leading cause of maternal morbidity and mortality. Worldwide, at least 200,000 maternal deaths are caused each year. Symptoms of preeclampsia usually appear after the 20th week of pregnancy and are usually detected by routine monitoring of a woman's blood pressure and urine. However, these monitoring methods cannot effectively diagnose early syndrome. If preeclampsia can be detected early and effective treatment can be taken, the risk of the subjects or the developing fetus can be reduced. [0003] The pathogenesis of preeclampsia is not yet fully understood, and its etiology may involve multiple factors such as maternal, placental, and fetal factors, i...

AI Technical Summary

Problems solved by technology

There are many factors involved in the pathogenesis, and the pathogenesis is more complicated, so the markers reported in the literature are also more and more mixed

[0009] Due to its complex pathogenesis, most of the commonly used markers in the current literature are single markers and combined detection of two markers. The combined detection of three markers is currently rare, and as far as reports are known, the selected markers Sensitivity and specificity need to be further improved after combined detection

In the selection of markers, there are also shortcomings such as poor representation and single selection.

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