Sequence and application of oligonucleotide ligand V4-2 for specifically recognizing Vasorin (VASN) protein

A technology of oligonucleotide ligands and nucleotide sequences, which is applied in the field of recognition of Vasorin protein, can solve problems such as nucleic acid aptamers that have not yet been seen, and achieve the goal of short cycle time, low preparation cost and broad market prospects Effect

Active Publication Date: 2015-03-25
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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Problems solved by technology

It has been identified that V4-2 can specifically recognize Vasorin (VASN) protein, but not bind to other proteins. The control nucleic acid sequence has no binding to the above proteins. There is no related research on nucleic acid aptamers that can specifically bind Vasorin (VASN) protein to report

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  • Sequence and application of oligonucleotide ligand V4-2 for specifically recognizing Vasorin (VASN) protein
  • Sequence and application of oligonucleotide ligand V4-2 for specifically recognizing Vasorin (VASN) protein
  • Sequence and application of oligonucleotide ligand V4-2 for specifically recognizing Vasorin (VASN) protein

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Embodiment Construction

[0012] The present invention will be further described below through the recognition of Vasorin (VASN) protein by V4-2.

[0013] The present invention is realized through the following technical solutions:

[0014] 1. The present invention is further described by the specific recognition of Vasorin (VASN) protein by isotope and biotin-labeled V4-2.

[0015] 1.1 synthesize V4-2 (SEQ ID No.1 in the sequence listing), GP35 (SEQ ID No.2 in the sequence listing) and GP45 (SEQ ID No.3 in the sequence listing) sequence, make V4-2, GP35 by displacement reaction and the 5' end of GP45 is tagged with γ- 32 P-ATP.

[0016] γ- 32 P-V4-2:

[0017] 5'-AGCAGCACAGAGGTCAGATGGTGGCTGGTGATGGGGGGCTGTATGCTGGTGTTGTATTGCCCTATGCGTGCTACCGTGAA-3'

[0018] γ- 32 P-GP35:

[0019] 5'-GCAATGGTACGGTACTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAGTGCACGCTACTTTGCTAA-3'

[0020] γ- 32 P-GP45:

[0021] 5’-GCAATGGTACGGTACTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAGTGCACGCTACTTTGCTAA-3’

[0022] Note: N...

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Abstract

The invention discloses a sequence and application of an oligonucleotide ligand V4-2 for specifically recognizing Vasorin (VASN) protein, belonging to the technical field of molecular biomedicine. The V4-2 capable of specifically recognizing the Vasorin (VASN) protein is detected by designing the specific sequence of V4-2 and adopting a 5'-end or 3'-end modification and marking (e.g. FITC (fluorescein isothiocyanate), biotin or digoxin) method. The V4-2 serving as a composition part of a kit or a detection index is used for auxiliary diagnosis and targeted therapy of liver cancer or basic research related with occurrence, development and progress of liver cancer diseases and the like. The method is simple, fast and sensitive, is suitable for auxiliary diagnosis of clinical tumor serum specimens and targeted biotherapy of tumors, and has a wide clinical application and basic application prospect.

Description

Field of invention: [0001] The invention relates to the sequence and modification of oligonucleotide ligand V4-2 specifically recognizing Vasorin (VASN) protein, and the application of the modified V4-2 in recognizing Vasorin (VASN) protein. The invention relates to the application of V4-2 in biomedicine. As a component or detection index of the kit, V4-2 is used for auxiliary diagnosis of liver cancer or basic research related to the occurrence, development and process of liver cancer. Application of V4-2 as a carrier to deliver drugs targeting liver cancer cells. Background technique: [0002] SELEX (Systematic Evolution of Ligands by EXponential Enrichment) technology is a high-throughput biological library screening technology that emerged and developed rapidly in the past ten years. Using large-capacity random oligonucleotide libraries (ssDNA library and RNA library), combined with PCR in vitro amplification technology, to exponentially enrich oligonucleotides that sp...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115G01N33/68
Inventor 邵宁生李少华李慧王玮丁红梅李洁黄皑雪赵强
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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