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Preparation method of bone peptide solution

A solution and osteopeptide technology, applied in the field of bioengineering, can solve the problems of low extraction rate of osteopeptide and rising production cost, and achieve the effects of significant curative effect, improved quality and improved degradation efficiency.

Inactive Publication Date: 2015-05-27
NANJING XINBAI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the production of osseopeptide by enzymatic hydrolysis, the active ingredients in the bone cannot be extracted to the maximum extent, resulting in a low extraction rate of osseopeptide and increased production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 20kg of healthy and fresh pig limb bones, wash them with warm water, crush them into bone pieces with a diameter of 0.5cm, put them in a container, add distilled water, add distilled water 3 times the weight of the bones, cook, and remove the upper layer of fat after standing for 1 hour. Move it into a high-pressure reactor, raise the temperature to 110°C, and maintain the pressure at 2.7kg / cm 2 , kept for 1.5 hours, and then decompressed to make the pressure in the kettle from 2.7kg / cm 2 Reduced to 0.5kg / cm 2 , the decompression process takes 2 hours, and then it is subjected to ultrasonic treatment, ultrasonic conditions: ultrasonic frequency is 26kHz, ultrasonic power is 300w, temperature is 40°C, and time is 20min. The purpose is to destroy the bone cell structure, dissolve the protein in the bone cell, and increase the yield. The filtrate was obtained by solid-liquid separation using a pipeline filter. Concentrate the filtrate to 50% of the original volume ...

Embodiment 2

[0027] Take 40kg of healthy and fresh bovine limb bones, wash them with warm water, crush them into bone pieces with a diameter of 1.5cm, put them in a container, add distilled water, add distilled water 4 times the weight of the bones, cook, and remove the upper layer of fat after standing for 1.5 hours. Move it into a high-pressure reactor, raise the temperature to 120°C, and maintain the pressure at 2.9kg / cm 2 , kept for 1.7 hours, and then decompressed to make the pressure in the kettle from 2.7kg / cm 2 Reduced to 0.8kg / cm 2 , the decompression process takes 2 hours, and then it is subjected to ultrasonic treatment, ultrasonic conditions: ultrasonic frequency is 30kHz, ultrasonic power is 290w, temperature is 42°C, and time is 15min. , using a pipeline filter for solid-liquid separation to obtain a filtrate. Concentrate the filtrate to 50% of the original volume to obtain a concentrate.

[0028] Keep the temperature of the concentrated solution at a constant temperature ...

Embodiment 3

[0031] Take 15kg each of healthy and fresh limb bones of pigs and sheep, 30kg in total, wash with warm water, crush them into 1.0cm in diameter bone pieces, put them in a container, add distilled water, add distilled water 5 times the weight of the bone, cook, let stand for 2.0 hours, then remove top layer of grease. Move it into a high-pressure reactor, raise the temperature to 120°C, and maintain the pressure at 3.0kg / cm 2 , kept for 2 hours, and then decompressed to make the pressure in the kettle from 3.0kg / cm 2 Reduced to 0.5kg / cm 2 , the decompression process takes 2.5 hours, and then it is subjected to ultrasonic treatment, ultrasonic conditions: ultrasonic frequency is 28kHz, ultrasonic power is 330w, temperature is 45°C, and time is 18min. The solid-liquid separation was carried out using a pipeline filter to obtain the filtrate. Concentrate the filtrate to 60% of the original volume to obtain a concentrate.

[0032] Keep the temperature of the concentrated soluti...

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PUM

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Abstract

The invention discloses a preparation method of a bone peptide solution and belongs to the field of biological engineering. The preparation method comprises the following steps: (1) crushing fresh limb bones of an animal, performing decompression extraction and ultrasonic treatment in a reaction kettle, extracting a concentrated solution and separating solid from liquid to obtain filtrate; (2) digesting by enzymolysis, namely respectively adding protease into the concentrated solution to perform enzymolysis; (3) heating the enzymolysis solution to realize enzyme deactivation, performing ultrafiltration and collecting ultrafiltrate to obtain the bone peptide solution. By crushing cells by adopting decompression extraction with assistance of ultrasonic wave, the extraction rate is increased; the method disclosed by the invention has the advantages of improving the degradation efficiency by adopting an enzymolysis method for production, reducing use of a great amount of acid, alkali and the like in a general extraction method, retaining active polypeptide in the bone peptide better and improving the product quality. The bone peptide product prepared by the method disclosed by the invention is good in quality, high in rate of finished products, remarkable in treatment effect, easy to operate and suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of osseopeptide solution, which specifically uses decompression, ultrasonic extraction and enzymatic hydrolysis to extract osseopeptide from limb bones of mammals, and belongs to the field of bioengineering. Background technique [0002] Osteopeptide is a kind of polypeptide mixture extracted from animal bones, containing inorganic elements such as calcium, phosphorus and iron, amino acid fragments and various bone growth factors, including bone protein peptide, bone blood protein peptide and bone collagen peptide and free amino acids, etc. Element. Due to the reasonable ratio of calcium, phosphorus and iron and the existence of various bone growth factors, osteopeptide has the functions of regulating bone metabolism, stimulating the proliferation of osteoblasts, promoting new bone formation, regulating calcium and phosphorus metabolism, increasing bone calcium deposition, and preventing osteoporosis, etc. ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 于辉沈飞杨文革李栋芸冯旌李向东程丽民朱文俊殷晶晶
Owner NANJING XINBAI PHARMA
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