A new method for making dog bone specimens

Abstract

The invention belongs to the technical field of specimen preparation, and in particular relates to a method for removing soft tissue from a canine bone specimen. The method includes removing the large muscle attached to the skeleton, dividing the skeleton into four parts: skull, spine, limb bones, sternum and ribs; preparing alkaline protease solutions with a concentration of 0.3% and 0.5%; dividing the skull, limb bones and Put the sternal ribs in 0.3% alkaline protease solution and soak them in a constant temperature incubator at 50°C for 36h, 66h, and 60h respectively; put the spine bones in a 0.5% alkaline protease solution and soak them in a constant temperature incubator at 50°C for 60h ; Take out the bone and rinse it with water. After the soft tissue is removed by the method of the present invention, the skeleton of the prepared specimen is complete, the residual oil of the skeleton is small, and the bone color of the prepared specimen is white.

Description

technical field [0001] The invention belongs to the technical field of specimen preparation, and in particular relates to a method for preparing a novel dog bone specimen. Background technique [0002] The steps of making canine bone specimens mainly include removing soft tissue, degreasing, bleaching, and imitating the normal and natural posture of animals for stringing. Among them, the removal of soft tissue is an important step. In the process of removing soft tissue, it is easy to cause bone damage, and the residual oil will cause the bone to turn black in subsequent production. The degree of soft tissue removal directly affects the subsequent degreasing time, bleaching effect and Skeletal integrity is the key to making high-quality canine bone specimens. Because the bone size and bone density of different species are different, the methods for removing soft tissue are also different. At present, the most commonly used method is to use low-concentration sodium hydroxide...

AI Technical Summary

Problems solved by technology

Because the bone size and bone density of different species are different, the methods for removing soft tissue are also different. At present, the most commonly used method is to use low-concentration sodium hydroxide to remove soft tissue and degrease, and sodium hydroxide solution is corrosive. Bones are prone to damage and require constant observation. Failure to master the concentration and time will cause irreversible damage to bone specimens. Improper operation will also cause damage to human skin. Therefore, it is necessary to improve the method of removing soft tissues from dog bone specimens. On the basis of improvement, perfect the degreasing and bleaching procedures to obtain a complete white bone

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