Method for extracting polyose from Hojothuria leucospilota, said polyose and its medicine use

A technology of sea cucumber and polysaccharides, which is applied in the field of extracting polysaccharides from jade ginseng, can solve the problems of not being able to effectively prevent and treat ischemic stroke and thrombotic diseases, and being unable to accurately control the intake of effective substances

Active Publication Date: 2008-11-12
黑龙江红豆杉药业有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, simply eating sea cucumbers cannot effectively prevent and treat ischemic stroke and thrombotic diseases. The reasons are: 1. The amount of active substances contained in different types of sea cucumbers and their composition are very different; 2. Simply eating sea cucumbers cannot accurately control the intake of active substances

Method used

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  • Method for extracting polyose from Hojothuria leucospilota, said polyose and its medicine use
  • Method for extracting polyose from Hojothuria leucospilota, said polyose and its medicine use
  • Method for extracting polyose from Hojothuria leucospilota, said polyose and its medicine use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, get Yuzu sea cucumber dry product 1000g, pulverize into powder (particle size is below 20 orders), with 10% NaHCO 3 Solution 8000ml, alkaline hydrolysis at 55-60°C for 8h; use 60mol / L HCL to adjust the pH to 8, add papain 10g, enzymolyze at 55-60°C for 10h, inactivate at 95°C for 30min, statically at 0-5°C Leave it overnight, take the clear liquid, adjust the pH to 2.0 with 6mol / L HCL, let it stand for 1h, take the clear liquid, adjust the pH to 8.0 with 1mol / L NaOH, and concentrate the clear liquid in a thin film evaporator to a relative density of 1.05- 1.10 (room temperature). Add ethanol to the concentrated solution to adjust the alcohol content to 50%, let it stand overnight, and collect the precipitate, which is the crude product.

[0019] Dissolve the crude product in an appropriate amount of water, adjust the pH to 8-9 with 1mol / L NaOH, add NaHSO 3 and 30%H 2 o 2 Decolorize and place in a refrigerator at 0-5°C overnight. The next day, take the...

Embodiment 2

[0021] 1. Test drug and configuration

[0022] (1) The ginseng polysaccharide powder obtained from Example 1 is prepared with 1% hydroxymethylcellulose sodium (CMC-Na), 10, 20, 40 mg / ml suspension;

[0023] (2) Aspirin: produced by Tianjin Lisheng Pharmaceutical Factory, batch number 971009, content 99.74%.

[0024] (3) Red tetracyanazole (TTC): Shanghai Reagent No. 3 Factory, batch number: 970410.

[0025] (4) Ferric chloride: Tianjin No. 3 Chemical Reagent Factory, batch number: 920410.

[0026] (5) Polymer dextran: Provided by Institute of Hematology, Chinese Academy of Medical Sciences, molecular weight: 300,000, 10% sterile saline solution, packaged in ampoules, 10ml each.

[0027] (6) Tianbaoning Ginkgo biloba leaves: produced by the Institute of Materia Medica, Chinese Academy of Sciences and Zhejiang Kangenbei Pharmaceutical Co., Ltd., batch number: 980601, each tablet contains 40 mg of Ginkgo biloba leaf extract. During the experiment, 40mg / ml and 5mg / ml suspension...

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Abstract

The present invention relates to method of extracting polysaccharide from Hojothuria leucosppilota. The method includes the steps of: alkaline hydrolysis of Hojothuria leucosppilota, enzymolysis, eliminating acid protein, alcohol precipitation, etc. The obtained Hojothuria leucosppilota polysaccharide product contains Hojothuria leucosppilota polysaccharide over 55 wt%. The Hojothuria leucosppilota polysaccharide has the functions of reducing cerebral ischemia, improving cerebral pia mater microcirculation, preventing platelet aggregation and thrombosis, and resisting coagulation, so that the Hojothuria leucosppilota polysaccharide may be used in preparing medicine for treating ischemic cerebral apoplexy and thrombotic diseases.

Description

technical field [0001] The invention relates to a method for extracting ginseng polysaccharide from yuzu sea cucumber, the ginseng polysaccharide itself and the medicinal use of the ginseng polysaccharide. Background technique [0002] Ischemic stroke and blood embolism are common and frequently occurring diseases, which seriously threaten human health. For the prevention and treatment of this type of disease, people have developed a variety of drugs. The field of traditional Chinese medicine has made its own contribution to this. According to the theory of traditional Chinese medicine, sea cucumber has the effects of nourishing yin and blood, promoting blood circulation and dredging collaterals, nourishing kidney and essence. However, simply eating sea cucumbers cannot effectively prevent and treat ischemic stroke and thrombotic diseases. The reasons are: 1. The amount of active substances contained in different types of sea cucumbers and their composition are very differ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/715A61P9/10
Inventor 陈任重王志国
Owner 黑龙江红豆杉药业有限责任公司
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