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Method for preparing antihypertensive peptides by utilizing probiotic fermented marine organism processing leftovers

A technology for marine organisms and blood pressure-lowering peptides, applied in the biological field, can solve problems such as no blood pressure-lowering peptides, and achieve good application prospects, social and economic benefits, and the effect of increasing added value.

Inactive Publication Date: 2015-06-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the preparation of antihypertensive peptides using collagen-rich marine bioprocessing scraps as raw materials, combined with protease liquefaction and probiotic fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A method for preparing hypotensive peptides by fermenting fish skin with probiotics, the specific process steps are as follows:

[0013] a. Selection and pretreatment of raw materials: select 10kg of fresh fish skin, wash it with clean water, remove and drain the water, add 100L of pure water and use a beater for beating treatment to obtain a slurry;

[0014] b. Protease liquefaction: heat the obtained slurry until the central temperature reaches 100°C, keep it for 10 minutes, kill microorganisms, cool to 50°C, adjust the pH to 5.2 with HCl, add 50g of papain for liquefaction treatment for 2 hours, and liquefy The solution was heated until the central temperature reached 100°C, and kept for 10 minutes to inactivate the enzyme activity to obtain an enzyme-inactivated liquefaction solution;

[0015] c. Preparation of the initial fermentation broth: add 2 kg of glucose to the obtained enzyme-inactivating liquefaction solution, and adjust the pH to 7.0 with NaOH to obtain t...

Embodiment 2

[0020] A method for preparing hypotensive peptides by fermenting squid skin with probiotics, the specific process steps are as follows:

[0021] a. Selection and pretreatment of raw materials: select 10kg of fresh squid skin, wash it with clear water, remove and drain the water, add 100L of pure water, and use a beater to perform beating treatment to obtain a slurry;

[0022] b. Protease liquefaction: heat the obtained slurry until the central temperature reaches 95°C, keep it for 10 minutes, kill microorganisms, cool to 55°C, add 50g of flavored protease for liquefaction treatment for 2 hours, and heat the liquefied liquid until the central temperature reaches 95°C Keep at ℃ for 10 minutes to inactivate the enzyme activity to obtain the liquefied solution of inactivated enzyme;

[0023] c. Preparation of the initial fermentation broth: add 1 kg of glucose to the resulting enzyme-inactivated liquefaction fluid, adjust the pH to 7.0, and obtain the initial fermentation broth; ...

Embodiment 3

[0028] A method for preparing hypotensive peptides by fermenting swim bladder with probiotics, the specific process steps are as follows:

[0029] a. Selection and pretreatment of raw materials: select 10kg of fresh fish maw, wash it with clean water, remove and drain the water, add 100L of pure water, and use a beater for beating treatment to obtain a slurry;

[0030] b. Protease liquefaction: heat the obtained slurry until the central temperature reaches 100°C, keep it for 10 minutes, kill microorganisms, cool to 55°C, add 50g flavored protease for liquefaction treatment for 2 hours, and heat the liquefied liquid until the central temperature reaches 100°C Keep at ℃ for 10 minutes to inactivate the enzyme activity to obtain the liquefied solution of inactivated enzyme;

[0031] c. Preparation of the initial fermentation broth: add 2 kg of glucose to the obtained enzyme-inactivating liquefaction solution, and adjust the pH to 7.0 with NaOH to obtain the initial fermentation b...

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Abstract

The invention relates to a method for preparing antihypertensive peptides by utilizing probiotic fermented marine organism processing leftovers. The method uses fish skin, squid skin, swimming bladder or fish scale and other collagen-enriched marine organism processing leftovers as raw materials, and comprises the following steps: washing and pulping; heating to kill microorganisms; cooling, and adding protease to perform liquefaction treatment; deactivating enzyme, adding glucose, and putting into a fermentation tank; sterilizing, inoculating a probiotic starter, fermenting and then removing residues; adding a cryoprotectant into the fermentation filtrate; freezing and drying to directly obtain a composite antihypertensive bio-preparation containing activated probiotics and bioactive peptides; furthermore, filtering the fermentation filtrate with a membrane, purifying, concentrating, and spray-drying to obtain the antihypertensive peptide product. By adopting the technical scheme combining protease liquefaction and probiotic fermentation, compared with a traditional enzyme preparation process, the method has the advantages that the preparation efficiency of high-activity antihypertensive oligopeptide can be improved, and the additional value of marine organism processing leftovers can be increased. The method is reasonable and effective in preparation process, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing blood pressure-lowering peptides by using probiotics to ferment marine biological processing leftovers, and belongs to the field of biotechnology. Background technique [0002] Hypertension is a chronic cardiovascular disease that plagues the world. It has a long incubation period and many complications. It requires long-term drug treatment to control the development of the disease. Although synthetic antihypertensive drugs have remarkable curative effects, they have produced various side effects in the process of clinical application. However, the antihypertensive functional factors present in natural animal and plant resources have the characteristics of safety, effectiveness, and no side effects, and have received widespread attention at home and abroad. Among them, antihypertensive peptides have become a hot spot in the research of prevention and treatment of hypertension. A large number of studies ...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P21/06C07K14/78C07K1/14A61P9/12C12R1/25C12R1/245C12R1/225C12R1/23C12R1/46C12R1/01
Inventor 周鹏刘大松刘小鸣胡锦华陈卫
Owner JIANGNAN UNIV
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