Extraction and preparation methods of swim bladder collagen oligopeptide

Abstract

The invention discloses extraction and preparation methods of a swim bladder collagen oligopeptide. The preparation method comprises the following specific steps: cleaning is conducted, specifically,a swim bladder is selected, cleaned, and crushed into small blocks, then a sodium hydroxide solution is added for soaking, washing is conducted with water till neutrality is achieved, then soaking isconducted through 5-10% of a n-butyl alcohol or isopropanol solution, washing is conducted with water till neutrality is achieved, and then slurry uniformizing is conducted for standby application; pretreatment is conducted, specifically, tannic acid is added into uniformized slurry, filtering is conducted, proteases are added under an alkaline environment for enzymolysis, and a mixture passes through a resin column; enzymolysis is conducted, specifically, the pH is adjusted, and 0.1-1.0% of compound proteases are added for enzymolysis; and purification is conducted, specifically, purificationcomprises membrane filtration, nanofiltration, concentration, sterilization and spray drying. According to the extraction and preparation methods, an extraction process is simple while the fishlike smell is effectively removed, and the swim bladder collagen oligopeptide is high in yield and good in quality.

Description

technical field

[0001] The invention belongs to the technical field of bioactive component extraction, and in particular relates to an extraction and preparation method of swim bladder collagen oligopeptide. Background technique

[0002] Active oxygen and free radicals are related to the pathogenesis of many human diseases such as cancer, aging, atherosclerosis, etc. Exogenous antioxidants help reduce the level of free radicals in the body, prevent lipid peroxidation, and help the body resist diseases. Although synthetic antioxidants such as BHA, BHT, TBHQ, and PG have good antioxidant effects, they have cumulative carcinogenic effects on human liver, spleen, and lungs; in recent years, natural antioxidant active peptides have strong activity and high Its safety has attracted the attention of scientific researchers and has become a research focus at home and abroad. Natural antioxidant peptides have relatively low molecular weight and are easily digested and absorbed by the...

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