Extraction and preparation methods of swim bladder collagen oligopeptide

A technology of swim bladder collagen and oligopeptides, which is applied in the field of bioactive component extraction, can solve the problems of unsuitability for large-scale production, high equipment requirements, complicated operation, etc., and achieve the effect of complete enzymatic hydrolysis, good profit, and the effect of reducing fishy smell

Active Publication Date: 2019-12-17
福州海锐黎思生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as one of the by-products, the swim bladder is rarely used or processed, and most of it is discarded as waste, resulting in a large waste of resources; the swim bladder is rich in colloid, with collagen as high as 80%, which can be used as a good and rich collagen Extracting resources, such as the application number CN105219827A discloses a preparation method of swim bladder collagen multifunctional peptide, which specifically includes cleaning, enzymatic hydrolysis, purification and other steps. After activity screening, the component with the strongest antioxidant activity is selected as the amino acid sequence of the target peptide , but this method requires high equipment and complicated operation, and is not suitable for large-scale production

Method used

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  • Extraction and preparation methods of swim bladder collagen oligopeptide

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Embodiment 1

[0022] A preparation method of swim bladder collagen oligopeptide, specifically comprising the following steps:

[0023] (1) Cleaning: Select the swim bladder for cleaning, break it into small pieces, add 0.1M sodium hydroxide solution according to the ratio of material to liquid 1:4, soak and stir constantly, wash with running water until neutral after 2 hours, and then press the material Add 5% isopropanol solution at a liquid ratio of 1:4 for soaking. After 12 hours, wash repeatedly with distilled water until neutral, then drain fully, then add deionized water at a ratio of solid to liquid of 1:4, homogenate, and set aside;

[0024] (2) Pretreatment: Add 0.5% tannic acid to the homogenate, stir for 45 minutes and filter, add 3 times the volume of deionized water to the obtained precipitate, and adjust the pH range to 7.8~ with sodium hydroxide Within the range of 8.5, add trypsin according to the amount of enzyme added at 0.05%, heat up to inactivate the enzyme after the pr...

Embodiment 2

[0032] A preparation method of swim bladder collagen oligopeptide, specifically comprising the following steps:

[0033] (1) Cleaning: Select the swim bladder for cleaning, break it into small pieces, add 0.1M sodium hydroxide solution according to the ratio of material to liquid 1:6, soak and stir constantly, wash with running water until neutral after 2 hours, and then press the material Add 5% isopropanol solution for soaking at a liquid ratio of 1:4, wash repeatedly with distilled water until neutral after 12 hours, then drain fully, then add deionized water at a ratio of 1:5 for solid-liquid ratio, homogenate, and set aside;

[0034] (2) Pretreatment: add 0.2% tannic acid to the homogenate, stir for 35 minutes and filter, add 3 times the volume of deionized water to the obtained precipitate, and adjust the pH range to 7.8~ with sodium hydroxide Within the range of 8.5, add trypsin according to the amount of enzyme added at 0.01%, heat up to inactivate the enzyme after the...

Embodiment 3

[0042] A preparation method of swim bladder collagen oligopeptide, specifically comprising the following steps:

[0043](1) Cleaning: Select the swim bladder for cleaning, break it into small pieces, add 0.1M sodium hydroxide solution according to the ratio of material to liquid 1:8, soak and stir constantly, wash with running water until neutral after 2 hours, and then press the material Add 5% isopropanol solution for soaking at a liquid ratio of 1:4. After 12 hours, wash repeatedly with distilled water until neutral, then fully drain, then add deionized water at a ratio of 1:6 for solid-liquid ratio, homogenate, and set aside;

[0044] (2) Pretreatment: add 0.6% tannic acid to the homogenate, stir for 50 minutes and filter, add 3 times the volume of deionized water to the obtained precipitate, and adjust the pH range to 7.8~ with sodium hydroxide Within the range of 8.5, add trypsin according to the amount of enzyme added at 0.08%, heat up to inactivate the enzyme after the...

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Abstract

The invention discloses extraction and preparation methods of a swim bladder collagen oligopeptide. The preparation method comprises the following specific steps: cleaning is conducted, specifically,a swim bladder is selected, cleaned, and crushed into small blocks, then a sodium hydroxide solution is added for soaking, washing is conducted with water till neutrality is achieved, then soaking isconducted through 5-10% of a n-butyl alcohol or isopropanol solution, washing is conducted with water till neutrality is achieved, and then slurry uniformizing is conducted for standby application; pretreatment is conducted, specifically, tannic acid is added into uniformized slurry, filtering is conducted, proteases are added under an alkaline environment for enzymolysis, and a mixture passes through a resin column; enzymolysis is conducted, specifically, the pH is adjusted, and 0.1-1.0% of compound proteases are added for enzymolysis; and purification is conducted, specifically, purificationcomprises membrane filtration, nanofiltration, concentration, sterilization and spray drying. According to the extraction and preparation methods, an extraction process is simple while the fishlike smell is effectively removed, and the swim bladder collagen oligopeptide is high in yield and good in quality.

Description

technical field [0001] The invention belongs to the technical field of bioactive component extraction, and in particular relates to an extraction and preparation method of swim bladder collagen oligopeptide. Background technique [0002] Active oxygen and free radicals are related to the pathogenesis of many human diseases such as cancer, aging, atherosclerosis, etc. Exogenous antioxidants help reduce the level of free radicals in the body, prevent lipid peroxidation, and help the body resist diseases. Although synthetic antioxidants such as BHA, BHT, TBHQ, and PG have good antioxidant effects, they have cumulative carcinogenic effects on human liver, spleen, and lungs; in recent years, natural antioxidant active peptides have strong activity and high Its safety has attracted the attention of scientific researchers and has become a research focus at home and abroad. Natural antioxidant peptides have relatively low molecular weight and are easily digested and absorbed by the...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC07K14/78C12P21/06
Inventor 陆晓丹皮俊飞
Owner 福州海锐黎思生物科技有限责任公司
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