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Process of utilizing sturgeon raw material to extract collagen

A collagen and raw material technology, applied in the field of aquatic product processing, can solve the problems of declining efficiency of aquaculture industry, limited market capacity, increased burden on enterprises, etc., to achieve the effect of improving degreasing efficiency and time, shortening removal time, and weakening rigid structure

Pending Publication Date: 2019-08-16
湖北清江鲟龙渔业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since 2000, the national sturgeon production has increased rapidly, but the market capacity is limited, resulting in a continuous decline in the price of commercial sturgeon fish and a decline in the efficiency of the aquaculture industry
In the process of processing, sturgeon skin is produced in the form of by-products, if it cannot be utilized, it will increase the burden on enterprises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The process for extracting collagen from sturgeon raw materials comprises the following steps:

[0029] 1) Select washed sturgeon skin as raw material, cut into cubes with a length of 5mm~10mm, and then add 5wt% sodium hydroxide solution to soak for 10 hours. The ratio of raw material to liquid of sturgeon is 1:10 ( g / mL), filter and wash the material after soaking;

[0030] 2) The material obtained in step 1) is added to a solution mixed with isopropanol, ethyl acetate and water at a weight ratio of 1:2:10 for degreasing treatment. The material-liquid ratio of the raw material and the degreasing mixed solution is 1:5 (g / mL), during the process of ultrasonic treatment, the ultrasonic frequency is 40KHz, the power is 300w, the treatment time is 2h, and it is cleaned after completion.

[0031] 3) Add the material obtained in step 2) to glacial acetic acid for swelling, then homogenize it, adjust the pH to 4.0-5.5 with sodium hydroxide solution, add papain and pepsin, ...

Embodiment 2

[0035] The process for extracting collagen from sturgeon raw materials comprises the following steps:

[0036] 1) Choose washed sturgeon skin as raw material, cut it into cubes with a length of 5mm~10mm, and then add it into a mixed solution of sodium hydroxide and sodium chloride for 10 hours, wherein the concentration of sodium chloride is 5wt%. Sodium hydroxide is also 5wt%, the ratio of solid to liquid of sturgeon raw material and solution is 1:10 (g / mL), stir during soaking, filter and wash the material after completion;

[0037] 2) The material obtained in step 1) is added to a solution mixed with isopropanol, ethyl acetate and water at a weight ratio of 1:2:10 for degreasing treatment. The material-liquid ratio of the raw material and the degreasing mixed solution is 1:5 (g / mL), during the process of ultrasonic treatment, the ultrasonic frequency is 40KHz, the power is 300w, the treatment time is 2h, and it is cleaned after completion.

[0038] 3) Add the material obt...

Embodiment 3

[0042] The process for extracting collagen from sturgeon raw materials comprises the following steps:

[0043] 1) Choose washed sturgeon skin as raw material, cut it into cubes with a length of 5mm~10mm, and then add it into a mixed solution of sodium hydroxide and sodium chloride to soak for 5 hours, wherein the concentration of sodium chloride is 10wt%. Sodium hydroxide is also 10wt%, the ratio of solid to liquid of sturgeon raw material and solution is 1:10 (g / mL), stir during soaking, filter and wash the material after completion;

[0044] 2) The material obtained in step 1) is added to a solution mixed with isopropanol, ethyl acetate and water at a weight ratio of 0.5:2:10 for degreasing treatment. The material-liquid ratio of the raw material and the degreasing mixed solution is 1:5 (g / mL), during the process of ultrasonic treatment, the ultrasonic frequency is 32KHz, the power is 300w, the treatment time is 2h, and it is cleaned after completion.

[0045] 3) Add the m...

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Abstract

The invention discloses a process of utilizing a sturgeon raw material to extract collagen. The process includes following steps: 1), selecting sturgeon skin and / or swim bladder which is washed cleanas a raw material, slicing, adding into 5-10wt% sodium hydroxide solution for soaking for 5-10h, filtering, and washing the material clean; 2), adding a material obtained in the step 1) into a defatting mixed solution for defatting for 1-2h, performing ultrasonic treatment during defatting, and washing clean after ultrasonic treatment is completed; 3), adding a material obtained in the step 2) into acid for swelling, homogenizing, using a sodium hydroxide solution to adjust pH to 4.0-5.5, adding protease for enzymolysis, adding yeast powder after enzymolysis is completed, controlling temperature at 30-35 DEG C, stirring for reaction for 2h, and performing enzyme deactivation; 4), discoloring enzymolysis liquid obtained after enzyme deactivation in the step 3), centrifuging, purifying, concentrating, and spray-drying to obtain collagen powder. The process can quickly extract collagen, and the collagen extracted by the process is high in purity, small in molecular weight and easy to absorb.

Description

technical field [0001] The invention belongs to the field of aquatic product processing, and relates to deep processing of sturgeon products, in particular to a process for extracting collagen by utilizing sturgeon raw materials. Background technique [0002] Sturgeon farming is an emerging industry in my country and has occupied a certain position in fishery production. With the pace of my country's agricultural product structure adjustment and the high profits of sturgeon farming, the sturgeon farming industry has gradually occupied an important position in my country's aquaculture industry and has become a new growth point in my country's freshwater aquaculture industry. It is a main direction of fishery industrial structure adjustment and fishermen's change of industry and industry, and the sturgeon aquaculture industry develops rapidly. [0003] Since 2000, the national sturgeon production has increased rapidly, but the market capacity is limited, resulting in a continu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Inventor 田开红田永康张书凡
Owner 湖北清江鲟龙渔业有限公司
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