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Preparation and preservation method of biovalve material treated by combination of double-bond polymerization and enzyme inhibitor

A biological valve and double-bond polymerization technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of limited source of pericardial material, inconvenient collection and transportation of raw materials, and improve anti-calcification performance and stability Effect

Inactive Publication Date: 2020-02-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porcine or bovine pericardial materials have limited sources, especially when there is a large-scale animal epidemic, and the collection and transportation of raw materials are inconvenient
Moreover, the bioprosthetic valve made of glutaraldehyde-crosslinked pericardium has a service life of only about 10 years due to problems such as calcification.

Method used

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  • Preparation and preservation method of biovalve material treated by combination of double-bond polymerization and enzyme inhibitor
  • Preparation and preservation method of biovalve material treated by combination of double-bond polymerization and enzyme inhibitor

Examples

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Effect test

Embodiment 1

[0029] A preferred embodiment of the present invention provides a method for the preparation and storage of a biological valve material treated with a combination of double bond polymerization and enzyme inhibitors. The specific steps are as follows:

[0030] a. Cut the swim bladder and decellularize it: place the swim bladder in a mixed solution of equal volumes of 0.5 wt% sodium lauryl sulfate and 0.5 wt% sodium deoxycholate for 24 hours at room temperature, and then wash it with sterile PBS solution Rinse in medium for 1 day.

[0031] b. Soak the decellularized fish swim bladder in glycidyl methacrylate solution and initiator for double bond polymerization and cross-linking: soak the decellularized fish swim bladder in 5wt% glycidyl methacrylate solution for 3 days at room temperature, then The double bond polymerization was thermally initiated at 37°C for 24 hours using a potassium persulfate initiator.

[0032] c. Soak the decellularized swim bladder in the glycosaminogl...

Embodiment 2

[0036] A preferred embodiment of the present invention provides a method for the preparation and storage of a biological valve material treated with a combination of double bond polymerization and enzyme inhibitors. The specific steps are as follows:

[0037] a. Cut the swim bladder and decellularize it: place the swim bladder in a mixed solution of equal volumes of 0.5 wt% sodium lauryl sulfate and 0.5 wt% sodium deoxycholate for 24 hours at room temperature, and then wash it with sterile PBS solution Rinse in medium for 1 day.

[0038] b. Soak the decellularized fish swim bladder in glycidyl methacrylate solution and initiator for double bond polymerization and cross-linking: soak the decellularized fish swim bladder in 5wt% glycidyl methacrylate solution for 3 days at room temperature, then The double bond polymerization was thermally initiated at 37°C for 24 hours using a potassium persulfate initiator.

[0039] c. Soak the decellularized swim bladder in a glycosaminoglyc...

Embodiment 3

[0043] A preferred embodiment of the present invention provides a method for the preparation and storage of a biological valve material treated with a combination of double bond polymerization and enzyme inhibitors. The specific steps are as follows:

[0044] a. Cut the swim bladder and decellularize it: place the swim bladder in a mixed solution of equal volumes of 0.5 wt% sodium lauryl sulfate and 0.5 wt% sodium deoxycholate for 24 hours at room temperature, and then wash it with sterile PBS solution Rinse in medium for 1 day.

[0045] b. Soak the decellularized fish swim bladder in glycidyl methacrylate solution and initiator for double bond polymerization and cross-linking: soak the decellularized fish swim bladder in 5wt% glycidyl methacrylate solution for 3 days at room temperature, then The double bond polymerization was thermally initiated at 37°C for 24 hours using a potassium persulfate initiator.

[0046] c. Soak the decellularized swim bladder in the glycosaminogl...

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Abstract

The invention discloses a preparation and preservation method of a biovalve material treated by combination of double-bond polymerization and an enzyme inhibitor. The preparation method comprises thefollowing steps that a, a swim bladder is cut and decellularized; b, the decellularized swim bladder is soaked in a glycidyl methacrylate solution and an initiator for double-bond polymerization and crosslinking; c, a glycosaminoglycase inhibitor is adopted for soaking; d, a mixed solution of carbodiimide and N-hydroxysuccinimide is adopted for crosslinking and fixation; and e, a bacteriostatic solvent is adopted for preservation after rinsing, or preservation is conducted after an alcoholic solution is adopted for dehydrating and drying. According to the prepared biovalve material, the stability and anti-calcification performance of glycosaminoglycan are improved, and a novel biovalve material with application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological valve materials, and in particular relates to a preparation and preservation method of a biological valve material treated in combination with double bond polymerization and enzyme inhibitors. Background technique [0002] The artificial bio-heart valve materials currently on the market are mainly made of pig or bovine pericardium as a raw material, and are prepared by cross-linking with glutaraldehyde solution. The source of pericardium material from pigs or cattle is limited, especially when there is a large-scale animal epidemic, the collection and transportation of raw materials are inconvenient. Moreover, the bioprosthetic valve made of glutaraldehyde-crosslinked pericardium has a service life of only about 10 years due to problems such as calcification. Based on this, the development of new biological valve materials with anti-calcification properties has important clinical value and soc...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/3687A61L27/50A61L2430/20A61L2430/40
Inventor 王云兵雷洋兰小蓉
Owner SICHUAN UNIV
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