Swim bladder source biological valve material as well as preparation method and application thereof

A technology of source organisms and swim bladders, applied in tissue regeneration, medical science, prosthesis, etc., to achieve the effects of good homogeneity, high yield, and avoiding insufficient service life

Active Publication Date: 2018-09-28
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that carp swim bladder has been used to successfully repair rabbit dura mate

Method used

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  • Swim bladder source biological valve material as well as preparation method and application thereof
  • Swim bladder source biological valve material as well as preparation method and application thereof
  • Swim bladder source biological valve material as well as preparation method and application thereof

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preparation example Construction

[0026] The invention provides a method for preparing a swim bladder-derived biological valve material, comprising the steps of:

[0027] S1 (decellularization of swim bladder): fresh bony fish swim bladders were preserved in PBS solution containing penicillin and streptomycin, and then placed in ice packs for later use; among them, bony fish include silver carp, grass carp, carp, yellow croaker, and sturgeon One or more of fish, Spanish mackerel and totoaba; in the PBS solution containing penicillin and streptomycin, the concentration of penicillin is 100U / mL, and the concentration of streptomycin is 0.1mg / mL;

[0028] Under sterile conditions, cut the swim bladder of bony fish, rinse it several times with sterile PBS solution, and then perform decellularization treatment, including the steps of: placing the swim bladder in 1% SDS solution at room temperature for 6-24 hours, and then transferring Treat in 1% Triton X-100 at room temperature for 0-120min, then soak and rinse in...

Embodiment 1

[0032] This embodiment provides a method for preparing a biological valve material derived from silver carp swim bladder, comprising steps:

[0033] S1 (decellularization treatment of silver carp swim bladder): fresh silver carp swim bladder was preserved in PBS solution containing penicillin and streptomycin, and then placed in ice pack for later use; in the PBS solution containing penicillin and streptomycin, the concentration of penicillin 100U / mL, the concentration of streptomycin is 0.1mg / mL;

[0034] Under sterile conditions, cut the silver carp swim bladder, and then rinse it several times with sterile PBS solution; then perform decellularization, including the steps: place the silver carp swim bladder in 1% SDS solution for 6 hours at room temperature, and then transfer Treat in 1% Triton X-100 at room temperature for 30 minutes, then soak and rinse in PBS solution for 48 hours, and then put it in a solution containing 0.2 mg / mL DNase and 20 mg / mL RNase for overnight d...

Embodiment 2

[0037] This embodiment provides a method for preparing a biological valve material derived from grass carp swim bladder, comprising steps:

[0038] S1 (grass carp swim bladder decellularization treatment): fresh grass carp swim bladder was stored in a PBS solution containing penicillin and streptomycin, and then placed in an ice pack for later use; in the PBS solution containing penicillin and streptomycin, the concentration of penicillin was 100U / mL, the concentration of streptomycin is 0.1mg / mL;

[0039] Under sterile conditions, the aortic valve of the grass carp swim bladder was cut, and then rinsed several times with sterile PBS solution; and then the grass carp swim bladder was decellularized, including the steps of: placing the grass carp swim bladder in 1% SDS solution for 6 hours at room temperature , then transferred to 1% Triton X-100 at room temperature for 30 minutes, then soaked and rinsed in PBS solution for 48 hours, and then placed in a solution containing 0....

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Abstract

The invention relates to a swim bladder source biological valve material as well as a preparation method and an application thereof. The preparation method comprises the following steps: cutting a swim bladder and conducting decellularization treatment on the swim bladder, so that a decellularized swim bladder is obtained; conducting immobilized cross-linking on the decellularized swim bladder, and rinsing the decellularized swim bladder obtained from cross-linking, so that the swim bladder source biological valve material is obtained. In comparison with existing bovine pericardium source biological valve materials, the swim bladder source biological valve material provided by the invention is smooth in both sides and good in uniformity of material thickness, and the swim bladder source biological valve material is nearly free from fat and other adhesion tissues, so that a raw material processing course becomes simpler to operate, and a yield of preparing biological valves from swim bladders is improved; and the swim bladder source biological valve material is equivalent with the bovine pericardium source biological valve materials in mechanical performance, however anti-calcification performance of the swim bladder material is much better than that of the bovine pericardium material, so that a shortcoming of bovine pericardium biological valves which are insufficient in service life can be overcome, and problems of the existing bovine pericardium source biological valve materials which are poor in durability, easy to cause calcification and the like can be solved.

Description

technical field [0001] The invention relates to the technical field of biological heart valves, in particular to a swim bladder-derived biological valve material and its preparation method and application. Background technique [0002] In the 1960s, biological valves emerged as the times require. They do not require long-term anticoagulant therapy and have excellent hemodynamic performance. In recent years, they have been used more and more in heart surgery valve replacement operations. Especially for elderly patients who are at risk of anticoagulation, biological valves are undoubtedly the best choice for heart valve replacement surgery. At the same time, as Transcatheter aortic valve replacement (TAVR) has become a hot new technology for valve treatment in the world, the artificial valve used in TAVR is a biological valve, which shows that the demand for biological valves will increase sharply in the future. surge. At present, most of the bioprosthetic valves used clinic...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/36
CPCA61L27/3604A61L27/3687A61L27/50A61L2430/20A61L2430/40A61L2430/36
Inventor 王志红刘婧秦怡博冷希岗孔德领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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