Antihypertensive peptide of swim bladder of large yellow croaker and preparation method and use thereof

A technology of antihypertensive peptide and large yellow croaker, which is applied in the preparation method of peptide, food preparation, chemical instruments and methods, etc., can solve the problems of preparation of antihypertensive peptide that have not been reported, and achieve the improvement of processing added value and significant effect of inhibiting ACE , no toxic side effects

Active Publication Date: 2014-08-13
ZHEJIANG OCEAN UNIV
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Problems solved by technology

[0004] However, the applicant found that the research on the preparation of antihypertensive enzymatic hydrolyzate of large yellow croaker swim bladder using enzymatic hydrolysis technology using large yellow croaker swim bla

Method used

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  • Antihypertensive peptide of swim bladder of large yellow croaker and preparation method and use thereof
  • Antihypertensive peptide of swim bladder of large yellow croaker and preparation method and use thereof
  • Antihypertensive peptide of swim bladder of large yellow croaker and preparation method and use thereof

Examples

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Embodiment 1

[0027] Example 1: A preparation method of a large yellow croaker swim bladder antihypertensive peptide, the preparation process is as follows: Large yellow croaker swim bladder "tissue crushed" buffer solution to extract crude protein "double enzyme enzymatic hydrolysis" enzymatic hydrolyzate "ultrafiltration" macroporous resin Segmented "Gel Filtration Chromatography" High Performance Liquid Chromatography Preparation of "Antihypotensive Peptides".

[0028] 1) Wash the swim bladder of large yellow croaker, crush and homogenate, add NaH according to the ratio of material to liquid 1g:10 ml 2 PO 4 -Na 2 HPO 4 Buffer solution (0.2mol / L, pH 7.0), shaking and extracting at 4 °C for 48 h, centrifuging at 12000 rpm for 15 min to obtain a supernatant; adding ammonium sulfate to the supernatant to make the ammonium sulfate concentration (weight / (volume percentage) reaches 30%, after standing for 1 h, centrifuge at 12000 rpm for 15 min to remove the precipitate, continue to add amm...

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Abstract

The invention discloses an antihypertensive peptide of a swim bladder of a large yellow croaker and a preparation method and use thereof. The amino acid sequence of the antihypertensive peptide is Leu-Arg-Pro-Ile(SEQ ID No:1). The molecular weight detected through ESI-MS (Electrospray Ionization Mass Spectrometry) is 497.65Da([M+H]+498.48Da). The preparation method comprises the following steps: washing the swim bladder of the large yellow croaker by distilled water, homogenizing by a high speed tissue stamp mill, extracting by a Na2HPO4-NaH2PO4 buffer liquid, and fractionally precipitating by ammonium sulfate to obtain a coarse protein; and sequentially carrying out enzymolysis on the coarse protein by trypsin and alkaline protease, centrifugalizing, ultrafiltering and chromatographically refining the zymolyte to obtain the antihypertensive peptide. The preparation process of the antihypertensive peptide disclosed by the invention is scientific and reasonable, the enzymolysis process by double enzymes is easy to control, and the prepared antihypertensive peptide has a remarkable inhibiting effect on the angiotensin converting enzyme (ACE), has the advantages of easy digestive absorption, safety, no toxic and side effects and the like and can be used as a medicine and a health-care food.

Description

technical field [0001] The invention relates to a fish active peptide and its preparation method and use, in particular to a large yellow croaker swim bladder antihypertensive peptide and its preparation method and use. Background technique [0002] Human blood pressure is regulated by many factors, the most important of which is the balance between the pressor system (renin-angiotensin system, RAS) and the depressor system (kallikrein-kinin system, KKS). Angiotensin-converting enzyme (ACE) is an important factor affecting the balance between the pressor system and the depressor system. ACE mainly raises human blood pressure in three ways: (1) cutting off the His-Leu at the carbon end of inactive angiotensin I (AngI), so that angiotensin I is converted into angiotensin II with strong vasoconstriction activity (AngⅡ), causing blood pressure to rise; (2) inactivating the hypotensive substance bradykinin in the KKS system, resulting in blood pressure rising; (3) stimulating th...

Claims

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Application Information

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IPC IPC(8): C07K5/103C07K1/30C07K1/36C07K1/34C07K1/20C07K1/16C12P21/06A61P9/12A23L1/29A23L33/00
Inventor 迟长凤王斌陈荫胡发远
Owner ZHEJIANG OCEAN UNIV
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