Jerusalem artichoke low-salt compound flavor pickle and preparation method thereof
A technology of Jerusalem artichoke and kimchi, which is applied in biochemical equipment and methods, microorganism-based methods, preservation of fruits and vegetables, etc., can solve the problems of complex starter preparation process, difficult to control fermentation process, black color and other problems, and achieve huge market development. Value, increase the added value of processing, the effect of fresh and natural smell
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Embodiment 1
[0050] Take the preserved strain of Lactobacillus pentosus glycerol, purchased from the China Common Microorganism Culture Collection Management Center, No. 1.3637, inoculate it on a fresh MRS solid plate by streaking, and activate it at 30°C for 24 hours; pick it up with a sterilized toothpick A single colony was inoculated into a test tube containing 10ml of modified MRS liquid medium, cultured at 30°C for 24 hours, and the cultured bacterial solution was transferred to a 500 ml tube containing 200ml of modified MRS liquid medium at a volume percentage of 2%. The Erlenmeyer flask was cultured at 30°C for 24 hours, centrifuged at 3000r / min for 5 minutes, the supernatant was removed, and the volume of sterilized water equal to the supernatant was added to resuspend the bacteria to prepare Lactobacillus pentosus seed solution for later use. The Lactobacillus plantarum preparation Paolamei-lactic acid bacteria powder II, purchased from Sichuan Gaofuji Biotechnology Co., Ltd., was...
Embodiment 2
[0056] Take the preserved strain of Lactobacillus pentosus glycerol, purchased from the China Common Microorganism Culture Collection Management Center, No. 1.3637, inoculate it on a fresh MRS solid plate, activate and cultivate it at 35°C for 36 hours; pick it up with a sterilized toothpick A single colony was inoculated into a test tube containing 10ml of modified MRS liquid medium, cultured at 35°C for 24 hours, and the cultured bacterial solution was transferred to a 500 ml tube containing 200ml of modified MRS liquid medium at a volume percentage of 2%. Erlenmeyer flask, culture at 35°C for 24 minutes, centrifuge at 3000r / min for 5 minutes, remove the supernatant, add the same volume of sterilized water as the supernatant to resuspend the bacteria, and prepare Lactobacillus pentosus seed solution for later use. The Lactobacillus plantarum preparation Paulome - Lactic Acid Bacteria Powder II was purchased from Sichuan Gaofuji Biotechnology Co., Ltd., added to sterilized wat...
Embodiment 3
[0062] Take the preserved strain of Lactobacillus pentosus glycerol (purchased from the China General Microorganism Culture Collection Management Center, No. 1.3637), inoculate it on a fresh MRS solid plate, activate and culture it at 30°C for 48 hours; pick it with a sterilized toothpick. Take a single colony and inoculate it into a test tube containing 10ml of modified MRS liquid medium, culture it at 25°C for 24h, transfer the cultured bacterial solution to a 500ml tube containing 200ml of modified MRS liquid medium at a volume percentage of 2%. ml Erlenmeyer flask, culture at 35°C for 24, centrifuge at 3000r / min for 5min, remove the supernatant, add the same volume of sterilized water as the supernatant to resuspend the bacteria, and make Lactobacillus pentosus seed liquid for later use. Add the Lactobacillus plantarum preparation Paulome—Lactic Acid Bacteria Powder II (purchased from Sichuan Gaofuji Biotechnology Co., Ltd.) into sterilized water at 35°C, then add 2% glucose,...
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