Production method for extracting chitosan oligosaccharide from shrimp shells

A production method and chitosan oligosaccharide technology are applied in the field of food processing, which can solve the problems of environmental pollution, waste of resources and the like, and achieve the effects of simple production equipment, low cost, and increased processing added value.

Inactive Publication Date: 2021-10-22
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shrimp shells are the main waste generated during shrimp processing. If they are thrown away as garbage, it will pollute the environment and waste resources.

Method used

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  • Production method for extracting chitosan oligosaccharide from shrimp shells
  • Production method for extracting chitosan oligosaccharide from shrimp shells
  • Production method for extracting chitosan oligosaccharide from shrimp shells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A method of producing shell oligosaccharides from a shrimp shell, specific steps:

[0064] (1) Step 1: Dry the shrimp shell at 50 ° C to constant weight, then pulverize the dried shrimp shell, over 80 mesh sieve, to obtain shrimp chopping powder;

[0065] (2) Step 2: Try 1 of the shrimp powder in step 1 to add 1.0 mol / L L-malic acid, which is 1 g: 10 mL, and after 20 min, centrifuge at 4000 rpm 5 min to give the precipitate. Drying to the constant weight to give a shrimp shell powder at a low temperature 50 ° C;

[0066] (3) Step 3: The step 2 is obtained by the decalcinated shrimp chrome; the mass ratio of shrimp chopper powder and water is 1 g: 14ml mixed paste, the pH is 7, and the mass concentration is 3% composite protease (medium) Protease: Alkaline protease is 3: 1), and the enzymatic hydrolysis was found to be found in 65 ° C, then the enzymatic hydrolyzed solution was centrifuged at 4000 rpm for 5 min, and precipitated, and the precipitate dried at a low temperat...

Embodiment 2

[0072] A method of producing shell oligosaccharides from a shrimp shell, specific steps:

[0073] (1) Step 1: Dry the shrimp shell at 50 ° C to constant weight, then pulverize the dried shrimp shell, over 80 mesh sieve, to obtain shrimp chopping powder;

[0074] (2) Step 2: Try 1 of the shrimp powder in step 1 to add 1.0 mol / L L-malic acid, which is 1 g: 10 mL, and after 20 min, centrifuge at 4000 rpm 5 min to give the precipitate. Drying to the constant weight to give a shrimp shell powder at a low temperature 50 ° C;

[0075] (3) Step 3: Section 2 to obtain a shrimp chopper powder; pressing the mass ratio of the shrimp shell and water to 1 g: 18ml mixed paste, adjusting pH 7, adding mass concentration of 4% composite protease (medium Protease: Alkaline protease is 3: 1), and the enzymatic hydrolysis is found to be 3.5 h under 60 ° C, then the enzymatic hydrolyzide is centrifuged at 4000 rpm for 5 min, and the precipitate is taken, and the precipitate is dried at a low temperat...

Embodiment 3

[0081] A method of producing shell oligosaccharides from a shrimp shell, specific steps:

[0082] (1) Step 1: Dry the shrimp shell at 50 ° C to constant weight, then pulverize the dried shrimp shell, over 80 mesh sieve, to obtain shrimp chopping powder;

[0083] (2) Step 2: Try 1 of the shrimp powder in step 1 to add 1.0 mol / L L-malic acid, which is 1 g: 10 mL, and after 20 min, centrifuge at 4000 rpm 5 min to give the precipitate. Drying to the constant weight to give a shrimp shell powder at a low temperature 50 ° C;

[0084] (3) Step 3: Section 2 to obtain a shrimp chopper powder; pressing the mass ratio of the shrimp shell and water to 1 g: 18ml mixed paste, adjusting pH 7, adding mass concentration of 4% composite protease (medium Protease: Alkaline protease is 3: 1), 55 ° C under conditions, and then centrifuge at 4000 rpm under 4000 rpm, and precipitate, dry the precipitate at a low temperature 50 ° C to constant weight Shrimp shell powder obtained by deprotein;

[0085] ...

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Abstract

The invention discloses a production method for extracting chitosan oligosaccharide from shrimp shells. The production method sequentially comprises the following steps: mashing, decalcifying, carrying out enzymolysis to remove protein, carrying out enzymolysis to extract sugar, concentrating, freeze-drying and the like to prepare the chitosan oligosaccharide. By means of the method, the chitosan oligosaccharide which is similar to commercially available food-grade chitosan oligosaccharide in the aspects of purity and average polymerization degree and higher in antioxidant activity can be obtained. The method not only effectively utilizes leftover shrimp shells and other wastes in shrimp processing, improves the processing added value of shrimp products, but also reduces environmental pollution, the technological process is green and pollution-free, the production equipment is simple, the cost is low, industrial production is easy to realize, and the method has very important significance for healthy development of the shrimp industry.

Description

Technical field [0001] The present invention relates to a production method of extracting shell oligosaccharides from a shrimp shell, belonging to the field of food processing. Background technique [0002] Chitosan is a natural cationic polysaccharide, which can be produced by a rich in natural circulation. Chitooligosaccharide, COS) is a polymerization obtained by D-amino glucose (or a small amount of N-acetyl-D-amino glucose) by β-1,4-glycoside linkage (DP) Low glycans at 2-20. Shell oligosaccharides As an important functional oligosaccharide, which has low toxicity, weak in vivo accumulation, and good water solubility. At the same time, it is also confirmed that important physiological activities such as immunization, liver protection and tumor suppression is an important new food raw material, which also has many related applications in many fields such as medicine, cosmetics, food and agriculture. In recent years, it has become a research hotspot in the field of food and he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/003C08B37/0003
Inventor 陈丽花曾文华蔡孝文
Owner SHANGHAI INST OF TECH
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