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Preparation method of swimming bladder bioprosthetic valve material and preservation method thereof

A biological valve and preservation method technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of inconvenient collection and transportation of raw materials, limited sources of pericardial materials, etc. Effect

Inactive Publication Date: 2020-02-07
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porcine or bovine pericardial materials have limited sources, especially when there is a large-scale animal epidemic, and the collection and transportation of raw materials are inconvenient
Moreover, the bioprosthetic valve made of glutaraldehyde-crosslinked pericardium has a service life of only about 10 years due to problems such as calcification.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation and storage method of a swim bladder biological valve material provided by a preferred embodiment of the present invention, the specific steps are as follows:

[0030] a. Cut the swim bladder and decellularize it: place the swim bladder in a mixed solution of equal volumes of 0.5 wt% sodium lauryl sulfate wt and 0.5 wt% sodium deoxycholate for 24 hours at room temperature, and then in sterile PBS Rinse in solution for 1 day.

[0031] b. Cross-linking and fixing the decellularized swim bladder with glutaraldehyde: soak the decellularized swim bladder in 0.1vt%, 0.5vt%, and 1vt% glutaraldehyde PBS solution for 24 hours to cross-link.

[0032] c. Post-crosslinking treatment with glycosaminoglycanase inhibitors: soak the glutaraldehyde-crosslinked swim bladder material in a PBS solution of 50 mM neomycin sulfate for 2 hours at room temperature.

[0033] d. Preserve with antibacterial solvent after rinsing or dehydrate and dry with alcohol solution: Soak the...

Embodiment 2

[0035] The preparation and storage method of a swim bladder biological valve material provided by a preferred embodiment of the present invention, the specific steps are as follows:

[0036] a. Cut the swim bladder and decellularize it: place the swim bladder in a mixed solution of equal volumes of 0.5 wt% sodium lauryl sulfate wt and 0.5 wt% sodium deoxycholate for 24 hours at room temperature, and then in sterile PBS Rinse in solution for 1 day.

[0037] b. Cross-linking and fixing the decellularized swim bladder with glutaraldehyde: soak the decellularized swim bladder in 0.1vt%, 0.5vt%, and 1vt% glutaraldehyde PBS solution for 24 hours to cross-link.

[0038] c. Use polyphenolic compounds and ferric chloride for cross-linking post-treatment: soak the swim bladder material cross-linked by glutaraldehyde in 50mM curcumin PBS solution at room temperature for 2h, and then soak in 1wt% ferric chloride aqueous solution Middle 2h.

[0039] d. Preserve with antibacterial solvent...

Embodiment 3

[0041]The preparation and storage method of a swim bladder biological valve material provided by a preferred embodiment of the present invention, the specific steps are as follows:

[0042] a. Cut the swim bladder and decellularize it: place the swim bladder in a mixed solution of equal volumes of 0.5 wt% sodium lauryl sulfate wt and 0.5 wt% sodium deoxycholate for 24 hours at room temperature, and then in sterile PBS Rinse in solution for 1 day.

[0043] b. Cross-linking and fixing the decellularized swim bladder with glutaraldehyde: soak the decellularized swim bladder in 0.1vt%, 0.5vt%, and 1vt% glutaraldehyde PBS solution for 24 hours to cross-link.

[0044] c. Post-crosslinking treatment with glycosaminoglycanase inhibitors: Soak the glutaraldehyde-crosslinked swim bladder material in a PBS solution of 50 mM neomycin and 5 mg / mL proanthocyanidins at room temperature for 2 hours.

[0045] d. Preserve with antibacterial solvent after rinsing or dehydrate and dry with alcoh...

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Abstract

The invention discloses a preparation method of a swimming bladder bioprosthetic valve material and a preservation method thereof. The preparation method comprises the following steps: a, cutting anddecellularizing swimming bladders; b, carrying out cross-linking immobilization on the decellularized swimming bladders by adopting glutaraldehyde; c, carrying out cross-linking post-treatment by adopting a polyphenol glycosaminoglycan enzyme inhibitor or carrying out cross-linking post-treatment by adopting a polyphenol compound and ferric chloride; and d, after rinsing, preserving by adopting anantibacterial solvent or preserving after dehydrating and drying by adopting an alcoholic solution. According to the swimming bladder bioprosthetic valve material prepared by the invention, the stability and the anti-calcification performance of glycosaminoglycan are improved, and the swimming bladder bioprosthetic valve material is the novel bioprosthetic valve material with an application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological valve materials, and in particular relates to a method for preparing and preserving a swim bladder biological valve material. Background technique [0002] The artificial bio-heart valve materials currently on the market are mainly made of pig or bovine pericardium as a raw material, and are prepared by cross-linking with glutaraldehyde solution. The source of pericardium material from pigs or cattle is limited, especially when there is a large-scale animal epidemic, the collection and transportation of raw materials are inconvenient. Moreover, the bioprosthetic valve made of glutaraldehyde-crosslinked pericardium has a service life of only about 10 years due to problems such as calcification. Based on this, the development of new biological valve materials with anti-calcification properties has important clinical value and social significance. Contents of the invention [0003] The purpose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/3641A61L27/3683A61L27/3687A61L27/50A61L2430/20
Inventor 兰小蓉王云兵雷洋
Owner SICHUAN UNIV
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