Preparation of boron-doped graphene quantum dot electrochemiluminescence sensor for detecting miRNA-20a and application of sensor

A graphene quantum dot, miRNA-20a technology, applied in the field of environmental analysis, can solve the problems of complex operation, poor practicability, cumbersome and time-consuming, etc., and achieve the effects of simple operation, strong practicability and high sensitivity

Active Publication Date: 2015-07-08
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention solves the shortcomings of the existing miRNA-20a detection method such as complicated operation, tedious time-consuming, high cost and poor practicability, and provides a simple, fast and practical quantitative analysis method of miRNA-20a

Method used

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  • Preparation of boron-doped graphene quantum dot electrochemiluminescence sensor for detecting miRNA-20a and application of sensor
  • Preparation of boron-doped graphene quantum dot electrochemiluminescence sensor for detecting miRNA-20a and application of sensor
  • Preparation of boron-doped graphene quantum dot electrochemiluminescence sensor for detecting miRNA-20a and application of sensor

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Experimental program
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Embodiment 1

[0027] The preparation of electrochemiluminescence probe BGQDs-DNA comprises the following steps:

[0028] (1) Add 23ml of concentrated sulfuric acid to 1g of high-purity graphite powder in an ice bath and stir, then add 3g of potassium permanganate to the above mixture in multiple times, and continue stirring for 24 hours. Add 50ml of water and let stand for 15 minutes, then add 150ml of water and 10ml of H 2 o 2 Stop the reaction. The mixture was centrifuged, and the precipitate was washed with 5% hydrochloric acid and water, respectively, to finally obtain graphite oxide.

[0029] (2) add 0.002g H in the graphite oxide that step (1) obtains 3 BO 3, sonicate for 10 minutes to make it evenly mixed. The mixture was then transferred to a glass tube and heated to 80 °C for 12 hours in a forced air oven. The product was washed several times with water, then vacuum freeze-dried, and finally boron-doped graphene rods were obtained.

[0030] (3) The boron-doped graphene rod o...

Embodiment 2

[0033] The preparation of electrochemiluminescence probe BGQDs-DNA comprises the following steps:

[0034] (1) Add 30ml of concentrated sulfuric acid to 1.2g of high-purity graphite powder in an ice bath and stir, then add 1.8g of potassium permanganate to the above mixture in multiple times, and continue stirring for 24 hours. Add 100ml of water and let stand for 15 minutes, then add 180ml of water and 15ml of H 2 o 2 Stop the reaction. The mixture was centrifuged, and the precipitate was washed with 5% hydrochloric acid and water, respectively, to finally obtain graphite oxide.

[0035] (2) add 0.0025g H in the graphite oxide that step (1) obtains 3 BO 3 , sonicate for 10 minutes to make it evenly mixed. The mixture was then transferred to a glass tube and heated to 80 °C for 12 hours in a forced air oven. The product was washed several times with water, then vacuum freeze-dried, and finally boron-doped graphene rods were obtained.

[0036] (3) The boron-doped graphen...

Embodiment 3

[0039] Configure the determination of miRNA-20a content in the sample:

[0040] (1) Add 25ml of concentrated sulfuric acid to 1.2g of high-purity graphite powder in an ice bath and stir, then add 1.5g of potassium permanganate to the above mixture in multiple times, and continue stirring for 24 hours. Add 45ml of water and let stand for 15 minutes, then add 200ml of water and 15ml of H 2 o 2 Stop the reaction. The mixture was centrifuged, and the precipitate was washed with 5% hydrochloric acid and water, respectively, to finally obtain graphite oxide.

[0041] (2) add 0.0045g H in the graphite oxide that step (1) obtains 3 BO 3 , sonicate for 10 minutes to make it evenly mixed. The mixture was then transferred to a glass tube and heated to 80 °C for 12 hours in a forced air oven. The product was washed several times with water, then vacuum freeze-dried, and finally boron-doped graphene rods were obtained.

[0042] (3) The boron-doped graphene rod obtained in step (2) i...

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Abstract

The invention belongs to the field of biology and relates to preparation of a boron-doped graphene quantum dot electrochemiluminescence sensor for detecting miRNA-20a and application of the sensor. The preparation method comprises the following steps: dropwise adding nanogold and BGQDs-DNA into a platinum electrode, so that the volumes of the nanogold and BGQDs-DNA on the electrode surface are 2-8 mu L / mm<2> and 2-6 mu L / mm<2>; and drying at room temperature, immersing the electrode into a buffer solution containing to-be-detected miRNA-20a of different concentrations, reacting at the temperature of 37 DEG C for 0.5-3 hours, and cleaning the electrode by using PBS buffer solution. The electrochemiluminescence signal of the electrode is detected by using a multi-channel chemiluminescence detection system, the electrolyte refers to 0.1M PBS and contains 0.1M KCl and 0.05M K2S2O8, the voltage is 0.7-1.15V, the scanning rate is 0.1V / s, and the high voltage of a photomultiplier is 600V. Compared with the traditional analysis method, the electrochemiluminescence detection method disclosed by the invention is low in cost, easy to operate and high in practicality.

Description

technical field [0001] The invention belongs to the field of environmental analysis, and relates to the preparation and application of a boron-doped graphene quantum dot electrochemical luminescence sensor for detecting miRNA-20a. Background technique [0002] miRNAs are a class of endogenous non-protein-coding RNA molecules with a length of about 20-25 nucleotides, which can participate in the regulation of various biological behaviors, such as cell differentiation, developmental processes, apoptosis, diseases, tumors, etc. Studies have shown that 50% of miRNAs are located in tumor-associated genome regions or vulnerable sites, and the expression of different types of miRNAs also indicates different tumor diseases. The expression of miRNA-20a has been proven to be associated with at least three solid tumors . Therefore, the quantitative detection of miRNA-20a plays a vital role in the clinical diagnosis of tumors and the evaluation of drug effects. [0003] Traditional mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N21/76
Inventor 赵慧敏张婷婷李亚璇李莉范高峰全燮
Owner DALIAN UNIV OF TECH
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