Microbial flora for preventing and treating wheat scab and separation method thereof

A technology of microbial flora and wheat scab, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unsatisfactory repair effects, achieve quick results in field application, easy large-scale promotion, Separation to obtain low-cost effects

Inactive Publication Date: 2020-07-24
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Traditional studies on the use of microorganisms to control wheat head blight mainly focus on the isolation of a single microbial strain, and the isolated effective control single bacteria is made into a microbial a...

Method used

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  • Microbial flora for preventing and treating wheat scab and separation method thereof
  • Microbial flora for preventing and treating wheat scab and separation method thereof
  • Microbial flora for preventing and treating wheat scab and separation method thereof

Examples

Experimental program
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Effect test

example 1

[0035] Example 1: Isolation, identification and biocontrol effect verification of microbial flora:

[0036] Preparation of Fusarium graminearum spore liquid:

[0037] The activated Fusarium graminearum was transferred to multiple PDA plates, cultured for 5 days at 25°C, picked mycelia into mung bean medium, cultured in a light shaker at 25°C, 180 rpm for 5 days, filtered through gauze, The filtrate was centrifuged at 7000 rpm for 10 min, and the precipitate was resuspended in sterile water at a concentration of 10 5 cells / mL, put in a refrigerator at 4°C for later use.

[0038] Isolation, identification and biocontrol effect verification of microbial flora:

[0039] Straw material pretreatment: Infected straw was collected from the wheat field in Nanjing City, Jiangsu Province, crushed to powder, air-dried naturally, and set aside.

[0040]Isolation of microbial flora: Put 100 mL of sterile inorganic salt medium into a 250 mL Erlenmeyer flask, add 5% of the straw into the E...

Embodiment 2

[0044] Embodiment two, field application

[0045] Expanded culture of microbial flora:

[0046] Straw material pretreatment: Infected straw was collected from the wheat field in Nanjing City, Jiangsu Province, crushed to powder, air-dried naturally, and set aside.

[0047] Isolation of microbial flora: Put 100 mL of sterile inorganic salt medium into a 250 mL Erlenmeyer flask, add 5% of the straw into the Erlenmeyer flask by liquid volume, and incubate on a shaker at 30°C at 180 rpm for 48 h. The flora suspension was transferred to a fresh 100 mL sterile inorganic salt medium at 10% of the liquid volume, continued to be cultured for 48 h, and subcultured 5 times according to the above method.

[0048] Preparation of fermentation broth: 400 L of inorganic salt medium was loaded into a 500 L fermenter, sterilized at 121 °C and 0.1 MPa for 30 min, and cooled to room temperature.

[0049] Expansion culture of the flora: add the treated stalks into the fermenter according to 5% o...

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Abstract

The invention discloses a microbial flora for preventing and treating wheat scab. The microbial flora mainly comprises two major components, namely bacteria and actinomycetes, wherein the identified bacterial components also comprise bacillus, pseudomonas, pantoea, bifidobacterium, bacillus aceticus, lactobacillus, stenotrophomonas, klebsiella, citrobacter and flavobacterium. The microbial flora has a relatively strong prevention and control effect on wheat scab and gibberellin thereof; the microbial flora is wide in raw material source, low in production and preparation cost, quick in effectin field application and free of secondary pollution to the environment; and the microbial flora disclosed by the invention is separated from infected straws, and is relatively strong in adaptabilityto a complex repair environment in the field and relatively significant in repair effect.

Description

technical field [0001] The invention belongs to the field of composite flora, and in particular relates to a microbial flora for preventing and treating wheat scab and an isolation method thereof. Background technique [0002] Wheat head blight (Fusarium head blight, FHB) is a common disease in wheat fields caused by one or more fungi, which can cause ear rot of wheat and seriously affect wheat yield. In the eastern part of my country, Fusarium graminearum is the main pathogen that induces wheat scab, and its infection process mainly occurs in the flowering stage; the climate characteristics of the flowering stage are: warmer weather, abundant rain, and high humidity in the air, which is very suitable for Fungal growth; infestation fungi are often spread in the field by wind or rain, and spread from the roots of the plants to the ears. After the wheat harvest of that year is completed, the pathogenic bacteria still remain in large quantities in the weeds, straw and soil, and...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01N63/22A01N63/27A01N63/20A01P3/00C12R1/07C12R1/38C12R1/22C12R1/20C12R1/02C12R1/01
CPCA01N63/00C12N1/02C12N1/20
Inventor 杨洪杏赵建荣项进王翔张浩曹胜飞马婷婷韩振周椿富程嘉蓉鲍文梅程嘉慧
Owner ANHUI SCI & TECH UNIV
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