Preparation method and application of dye decolorizing complex microbial inoculant

A compound bacterial agent and dye decolorization technology, which is applied in the field of preparation of dye decolorizing compound bacterial agent, can solve the problems of limiting the industrial application of dye-degrading bacteria, having great influence on bacterial decolorization ability, and unsatisfactory decolorization effect, and achieving good decolorization and degradation ability. , the effect of strengthening decolorization and degradation ability, good application prospects and ecological benefits

Inactive Publication Date: 2019-02-22
QIANNAN NORMAL UNIV FOR NATTIES
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of bacteria have been screened out in the natural environment with the ability to decolorize and degrade, such as: Rhodococcus (Rhodococcus), Pseudomonas (Pseudomonas), Bacillus (Bacillus), Lysinibacillus ( Lysinibacillus), Sphingobacterium (Sphingobacterium), etc., but their decolorization effect is not ideal
Environmental factors (such as temperature, pH and salt concentration, etc.) have a great influence on the decolorization ability of bacteria, which greatly limits the industrial application of dye-degrading bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of dye decolorizing complex microbial inoculant
  • Preparation method and application of dye decolorizing complex microbial inoculant
  • Preparation method and application of dye decolorizing complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method for dye decolorization composite bacteria agent, comprising the following steps:

[0034] (1) Take the mangrove sea mud, screen and separate the bacteria in the mangrove sea mud, and obtain Citrobacter H-3 and Novosphingobium TH-5 with strong dye decolorization ability and Paenibacillus TH-7;

[0035] (2) Inoculate Citrobacter H-3, Novosphingobium TH-5 and Paenibacillus TH-7 isolated in step (1) into liquid LB medium respectively Carry out aerobic culture, and obtain Citrobacter liquid, Neosphingobacterium liquid and Paenibacillus liquid respectively after the culture is completed; the temperature of the aerobic culture is 28°C, the rotational speed is 130rpm, and cultivated under aerobic conditions for 12h;

[0036] (3) Equal volumes of the Citrobacter liquid, Neosphingobacterium liquid and Paenibacillus liquid obtained in step (2) are inoculated in the mixed medium of liquid LB and dyes for fermentation and cultivation, and then domesticated, and...

Embodiment 2

[0041] A preparation method for dye decolorization composite bacteria agent, comprising the following steps:

[0042] (1) Take the mangrove sea mud, screen and separate the bacteria in the mangrove sea mud, and obtain Citrobacter H-3 and Novosphingobium TH-5 with strong dye decolorization ability and Paenibacillus TH-7;

[0043] (2) Inoculate Citrobacter H-3, Novosphingobium TH-5 and Paenibacillus TH-7 isolated in step (1) into liquid LB medium respectively Carry out aerobic culture, and obtain Citrobacter liquid, Neosphingobacterium liquid and Paenibacillus liquid respectively after the culture is completed; the temperature of the aerobic culture is 32°C, the rotational speed is 180rpm, and cultivated under aerobic conditions for 24h;

[0044] (3) Equal volumes of the Citrobacter liquid, Neosphingobacterium liquid and Paenibacillus liquid obtained in step (2) are inoculated in the mixed medium of liquid LB and dyes for fermentation and cultivation, and then domesticated, and...

Embodiment 3

[0049] A preparation method for dye decolorization composite bacteria agent, comprising the following steps:

[0050] (1) Take the mangrove sea mud, screen and separate the bacteria in the mangrove sea mud, and obtain Citrobacter H-3 and Novosphingobium TH-5 with strong dye decolorization ability and Paenibacillus TH-7;

[0051] (2) Inoculate Citrobacter H-3, Novosphingobium TH-5 and Paenibacillus TH-7 isolated in step (1) into liquid LB medium respectively Carry out aerobic culture, and obtain Citrobacter liquid, Neosphingobacterium liquid and Paenibacillus liquid respectively after the cultivation is completed; the temperature of the aerobic culture is 29° C., the rotational speed is 140 rpm, and cultivated under aerobic conditions for 15 hours;

[0052] (3) Equal volumes of the Citrobacter liquid, Neosphingobacterium liquid and Paenibacillus liquid obtained in step (2) are inoculated in the mixed medium of liquid LB and dyes for fermentation and cultivation, and then domes...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
decolorization rateaaaaaaaaaa
decolorization rateaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method of dye decolorizing complex microbial inoculant. The method comprises the following steps: separating mangrove sea mud to obtain Citrobacter H-3, Novosphingobium TH-5 and Paenibacillus TH-7 with relatively strong dye decolorizing performance, and respectively culturing to obtain bacterial liquids; and mixing the bacterial liquids, culturing and domesticating to obtain the complex microbial inoculant. The complex microbial inoculant has excellent decolorizing and degrading performance on simulated dyeing wastewater, is simple in preparation method and low in cost, can widely grow in a pH and saline environment, and has decolorizing and degrading performance on various dyes. Compared with single bacterium, the complex microbial inoculant has the advantage that the decolorizing effect is remarkably improved. Due to synergistic combination of Citrobacter, Novosphingobium and Paenibacillus, the dye in the dyeing wastewater can be quickly decolorized and degraded by the complex microbial inoculant, and secondary pollution cannot be caused.

Description

technical field [0001] The invention belongs to the technical field of printing and dyeing wastewater treatment, and in particular relates to a preparation method and application of a dye decolorization composite bacterial agent. Background technique [0002] In recent years, with the rapid development of textile and printing and dyeing industries, the number and variety of chemically synthesized dyes have been increasing. About 20-30% of dyes are released into the environment during production and use, and the environmental pollution caused by printing and dyeing wastewater is becoming more and more serious. Synthetic dyes are mainly artificially synthesized aromatic compounds, which have stable structure and have the characteristics of anti-acid and alkali, anti-microbial and anti-photodegradation, etc. The wastewater containing dyes in the discharge and environment has potential three effects on organisms (carcinogenic, teratogenic, Mutagenic); therefore, the development...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/01C02F103/30C02F101/30
CPCC12N1/20C12N1/36C02F3/341C02F2103/30C02F2101/308
Inventor 毛海立李锋徐平黄德娜
Owner QIANNAN NORMAL UNIV FOR NATTIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap