Enhanced high-efficiency nitrogen-fixing complex microbial system added with non-nitrogen-fixing bacteria and application thereof

A nitrogen-fixing bacteria and enhanced technology, applied in the field of agricultural microorganisms, can solve the problem of not considering the synergistic effect of nitrogen-fixing bacteria, etc., and achieve the effects of improved root growth, strong environmental adaptability, and good corn growth.

Active Publication Date: 2021-08-20
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the above inventions considered the synergistic effect between nitrogen...

Method used

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  • Enhanced high-efficiency nitrogen-fixing complex microbial system added with non-nitrogen-fixing bacteria and application thereof
  • Enhanced high-efficiency nitrogen-fixing complex microbial system added with non-nitrogen-fixing bacteria and application thereof
  • Enhanced high-efficiency nitrogen-fixing complex microbial system added with non-nitrogen-fixing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Construction of Enhanced High Efficiency Nitrogen Fixing Compound Bacteria and Verification of Nitrogen Fixation Effect

[0042] 1. Screening of strains in complex strains

[0043] 1) The juices from the stems of five maize varieties, Kenwo 6, Liangyu 66, Jundan 20, Zhengdan 958, and Yedan 13, were collected at the experimental base of the Institute of Agricultural Resources and Agricultural Regional Planning, Chinese Academy of Agricultural Sciences. Select the central position of the third stem above the base of the corn, and cut off the corn stem with pruning shears. Due to the pressure of the corn root, the juice of the conducting tissue of the corn stem will flow out from the cross-section, and use 0.5g sterile absorbent cotton balls to absorb the corn stem juice. For juice, put absorbent cotton balls into 50mL sterile centrifuge tubes, place them on ice and bring them back to the laboratory.

[0044] 2) Add 2 mL of 0.9% sterile normal saline to a centri...

Embodiment 2

[0077] Example 2 Application of Enhanced High Efficiency Nitrogen-fixing Compound Bacteria Lines in Maize Growth

[0078] 1) Select the corn variety Zhengdan 958, sterilize the corn seeds with 75% alcohol for 3 minutes, then sterilize them with 5% sodium hypochlorite for 8 minutes, and then wash them with sterile water for 3 times. Irrigate the quartz sand with bacterial water, and germinate in the dark at 30°C for 3 days in the incubator. After 3 days, transfer the germinated corn seeds to the artificial climate box, with 16 hours of light during the day, 8 hours of darkness at night, and an air humidity of 60%.

[0079] 2) After the corn grows three leaves, carefully pull out the corn seedling from the quartz sand, and remove the corn seed endosperm. The nitrogen-fixing bacteria group A and the non-nitrogen-fixing bacteria group B were mixed at a ratio of 1:1 to form an enhanced high-efficiency nitrogen-fixing compound bacteria solution. The corn roots were soaked in the com...

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Abstract

The invention discloses an enhanced high-efficiency nitrogen-fixing complex microbial system added with non-nitrogen-fixing bacteria and application thereof, and belongs to the technical field of agricultural microorganisms. The invention discloses an enhanced high-efficiency nitrogen-fixing bacteria, which comprises at least one of klebsiella MNAZ1050, citrobacter MNAZ1397 and pseudomonas MNAZ228; the invention further discloses an enhanced high-efficiency nitrogen-fixing complex microbial system which comprises nitrogen-fixing bacteria and non-nitrogen-fixing bacteria; wherein the nitrogen-fixing bacteria comprise at least one of the above three bacteria; the non-nitrogen-fixing bacteria comprises at least one of acinetobacter ACZLY512 and kluyveromyces AZ981. The nitrogen-fixing bacteria in the nitrogen-fixing complex microbial system have a better nitrogen-fixing effect when being independently used, and the nitrogen-fixing effect of the nitrogen-fixing complex microbial system constructed by cooperatively using the nitrogen-fixing bacteria and the non-nitrogen-fixing bacteria is more remarkable than that of the nitrogen-fixing bacteria when being independently used, so that crop growth can be promoted under the condition of obviously reducing nitrogen fertilizer application, and the nitrogen-fixing complex microbial system has important significance for improving the crop yield.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, and relates to an enhanced high-efficiency nitrogen-fixing composite bacterial line added with non-nitrogen-fixing bacteria and its application. Background technique [0002] Nitrogen fertilizer is a key factor for high and stable crop yield, contributing more than 50% to crop yield increase. However, when the world began to use nitrogen fertilizers on a large scale, especially in some countries and regions, the excessive use of nitrogen fertilizers led to a series of ecological and environmental problems, such as soil acidification, greenhouse gas emissions, water eutrophication, and excessive nitrate content in agricultural products. , seriously hampering the sustainable development of global agriculture. In this context, biological nitrogen fixation has become one of the important ways to reduce agricultural nitrogen fertilizer application. [0003] Biological nitrogen fi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/22C12R1/38C12R1/01
CPCC12N1/20C05F11/08C12N1/205C12R2001/22C12R2001/01C12R2001/38A01N63/20
Inventor 艾超张俪予周卫
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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