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Low-molecular-weight alfalfa polysaccharide as well as preparation method and application thereof in regulating intestinal flora

A kind of alfalfa polysaccharide, low molecular weight technology, used in drug combination, digestive system and other directions

Active Publication Date: 2021-04-30
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on alfalfa polysaccharides with low molecular weight and uniform monosaccharide content and their application in regulating intestinal flora

Method used

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  • Low-molecular-weight alfalfa polysaccharide as well as preparation method and application thereof in regulating intestinal flora
  • Low-molecular-weight alfalfa polysaccharide as well as preparation method and application thereof in regulating intestinal flora
  • Low-molecular-weight alfalfa polysaccharide as well as preparation method and application thereof in regulating intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Preparation of alfalfa polysaccharide

[0028] Step 1: Raw material pretreatment: Dry the alfalfa raw material in a blast drying oven at 60°C for 24 hours, crush it through a 40-mesh sieve, degrease it with petroleum ether until it is colorless, filter, evaporate the residue to dry petroleum ether, and dry it at 60°C for 24 hours , to get alfalfa dry powder.

[0029] Step 2: Extraction: Weigh 50 g of alfalfa dry powder, add pH 5 disodium hydrogen phosphate-citrate buffer according to the ratio of solid to liquid 1:30, then add 6% cellulase, extract alfalfa polysaccharide at 50°C for 40 min, and sterilize at 90°C The enzyme was activated for 10 min, centrifuged at 8000 r / min for 15 min, and the supernatant was collected. The supernatant was concentrated to 1 / 5 of the original volume with a rotary evaporator to obtain a concentrated alfalfa polysaccharide. Add 95% ethanol to the concentrate at a volume ratio of 1:4, ethanol precipitation for 24 h, centrifug...

Embodiment 2

[0039] The preparation of embodiment 2 alfalfa polysaccharides

[0040] The difference from Example 1 is that in step 2, a pH5 disodium hydrogen phosphate-citric acid buffer solution is added according to a solid-to-liquid ratio of 1:20, and then 8% cellulase is added.

[0041] The molecular weight of the prepared alfalfa polysaccharide was 3-5 KDa, and the monosaccharide composition included 1.16% mannose: 68.57% glucose: 4.53% galactose.

Embodiment 3

[0042] Example 3 Application of alfalfa polysaccharides to regulate intestinal flora

[0043] Preparation of human intestinal flora: Take fresh feces of the same quality from three volunteers with healthy intestinal tract and who have not taken antibiotics within 3 months, and immediately add D-PBS solution with a volume fraction of 10% after mixing to prepare a mass of The solid-liquid mixture with a fraction of 20% was stirred evenly with a sterile glass rod, filtered through four layers of sterile gauze, and the filtrate was collected to obtain the human intestinal flora.

[0044] In vitro fermentation: the alfalfa polysaccharide obtained in Example 1 was mixed evenly with the fermentation medium to prepare a culture solution with a concentration of alfalfa polysaccharide of 2%, and 9 mL / bottle was dispensed into anaerobic culture bottles and sterilized. Under anaerobic and aseptic conditions, add 1 mL of the human intestinal flora obtained in step 1 into the above-mentione...

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Abstract

The invention discloses low-molecular-weight alfalfa polysaccharide as well as a preparation method and application thereof in regulating intestinal flora, the molecular weight of the low-molecular-weight alfalfa polysaccharide is 3-6 KDa, and monosaccharide comprises 0.75-1.16% of mannose, 68.57-81.02% of glucose and 1.10-4.53% of galactose; The low-molecular-weight alfalfa polysaccharide is prepared by cellulase extraction, Sevage reagent deproteinization, cotton-wool-shaped DEAE-52 cellulose decoloration and granular DEAE-52 cellulose separation and purification. The low-molecular-weight alfalfa polysaccharide has the effects of regulating intestinal flora, promoting the growth of probiotics such as bifidobacterium and lactobacillus, inhibiting the growth of pathogenic bacteria such as citrobacter and escherichia coli, generating short-chain fatty acids such as acetic acid through glycolysis, and reducing the pH value of the intestinal tract.

Description

technical field [0001] The invention belongs to the technical field of plant polysaccharides, and in particular relates to a low-molecular-weight alfalfa polysaccharide, a preparation method thereof and an application for regulating intestinal flora. Background technique [0002] Alfalfa is a perennial herb belonging to the leguminous family Alfalfa, which is one of the most widely cultivated forage varieties in the world; alfalfa is also a traditional Chinese herbal medicine, which has the functions of clearing heat and detoxifying, cooling blood and treating stranguria, nourishing qi and strengthening spleen, warming kidney, and lowering blood fat. and other effects. Alfalfa contains bioactive components such as polysaccharides, saponins, flavonoids, and polyphenols. Among them, alfalfa polysaccharides have various pharmacological activities such as anti-oxidation, anti-inflammation, anti-tumor, and immune regulation, and can also be used to promote poultry production. It...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P1/00
CPCC08B37/006C08B37/0003A61P1/00
Inventor 李凤伟彭梦黎余晓红王笃军商曰玲闵晓冉朱袁平
Owner YANCHENG INST OF TECH
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