Citrobacter vinegaticus and application thereof in chloramphenicol removal and phosphorus and potassium dissolving

A technology of Citrobacter bacillus and chloramphenicol, which is applied in the field of microbial restoration of organic pollutants, can solve the problems of harsh reaction conditions, secondary pollution, and difficult to popularize, and achieves the effect of removing or residual and reducing residual.

Active Publication Date: 2021-11-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods commonly used to degrade chloramphenicol are mainly physical or chemical methods such as Fenton advanced oxidation method, photocatalytic reduction degradation method, microwave radiation, etc., but there are high processing costs, harsh reaction conditions, large energy consumption, and easy formation of secondary pollution. and other shortcomings, it is not easy to widely promote

Method used

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  • Citrobacter vinegaticus and application thereof in chloramphenicol removal and phosphorus and potassium dissolving
  • Citrobacter vinegaticus and application thereof in chloramphenicol removal and phosphorus and potassium dissolving
  • Citrobacter vinegaticus and application thereof in chloramphenicol removal and phosphorus and potassium dissolving

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Screening, separation and purification and identification of strain SZW2

[0058] First, chloramphenicol degradation strain SZW2 screening method

[0059] Material preparation

[0060] Sample Sample Sample Sample Sample Sample: From the dry water of Longping Town, Liyuan, Qingyuan, Guangdong Province, it has been sealed. Contents.

[0061] LB medium: 5.0 g of protein 胨, 5.0 g of yeast extract powder, NaCl 10.0 g, pH 7.0 ~ 7.2, distilled water to 1 L, solid medium additional 18.0 g agar powder. Determination of 121 ° C for 15 min.

[0062] Inorganic Salt Media (MSM): 5 ml of phosphate buffered solution (kh 2 PO 4 8.5g · L -1 K 2 HPO 4 · H 2 O21.75g ​​· L -1 NA 2 HPO 4 12h 2 O 33.4g · L -1 NH 4 CL 5.0g · L -1 ), 3.0ml 22.5g · L -1 MGSO 4 Solution (Mgso 4 · 7h 2 O 46.125g · L -1 ), 1.0ml 0.25g · L -1 FECL 3 Solution (FECL 3 · 6h 2 O 0.42g · L -1 ), 1.0ml 36.4g · L -1 CaCL 2 Solution (CaCl 2 · 2h 2 O 48.22g · L -1 ), 1.0ml trace element solution (including 39.9mg · L ...

Embodiment 2

[0084] Example 2 strain SZW2 removal of chloramphenicol test

[0085] The strain SZW2 scribe was cultured from 30 ° C for 20 hours of LB plate, and the proportion of 2% (V / V) was inoculated in a shaker of 30 ° C, 30 ° C, and 150 rpm. Chloramphenicol concentration is 10mg · L -1 Containing 10g · L -1 In inorganic salt liquid medium of protein, 30 ° C, 150R · min -1 Oscillating culture 0, 0.25, 0.5, 1, 2, 3, 4, 5, and 6 days (D), with a non-adding strain SZW2 as a blank control (CK), three repeated tests, determination of strain SZW2 on chloramphenicol Removal and removal efficiency; where CAP content is measured by HPLC (the method is in Example 1).

[0086] The strain SZW2 on chloramphenicol removal efficiency is 0, 5.79, 16.73, 21.55, 29.6, 30.95, 31.98, 35.04 and 36.80%. Strain SZW2 in 10g · L -1 The removal of chloramphenicol in the MSM medium, as well as the growth of strains and pH changes, image 3 As shown: SZW2 growth presents "S-type" curve, in 2 days (d), the maximum co...

Embodiment 3

[0087] Example 3 Phosphorus Potassium Potassium Potassium Test of Strain SZW2

[0088]Solicosphocetin (Phosphate / Potassium Solubilizing Bacteria) intended to convert an insoluble phosphorus and potassium substance into soluble phosphorus and potassium) (specific reference: rawat, p., DAS, S., Shankhdhar, D., et al, phosphate-solubilizing microorganisms:. Mechanism andtheir role in phosphate solubilization and uptake [J] .Journal of Soil Scienceand Plant Nutrition.2020, https: / / doi.org / 10.1007 / s42729- 020-00342-7.) Functional Test:

[0089] The strain SZW2 is inoculated in LB liquid medium, from 125 to 150r · min -1 28 ~ 30 ° C shaker oscillated into 18 to 24 h, after 2% (V / V) was connected to NBrip or pitcass bacteria liquid medium, and three repetition tests were placed at 30 ° C, 150R · min. -1 Culture in the shaker was cultured, determined the content of effective phosphorus and quick-fitting potassium in the solution (measured by the flame photometric meter of quick-actin...

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Abstract

The invention discloses citrobacter vinegaticus and application of the citrobacter vinegaticus in removal of chloramphenicol and dissolution of phosphorus and potassium. The name of the citrobacter vinegatus is Citrobacter portucalensis SZW2, the preservation number of the citrobacter vinegatus is GDMCC NO: 61695, and the citrobacter vinegatus is preserved in Guangdong Province Microbial Culture Collection Center on the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City on May 27, 2021. The Citrobacter portucalensis SZW2 can remove chloramphenicol in a co-metabolism mode and can secrete organic acid substances to reduce the pH value in a system, so insoluble phosphorus and potassium substances are dissolved, the content of available phosphorus and potassium in soil can be increased, and therefore, the Citrobacter portucalensis SZW2 can be used for removing chloramphenicol or promoting plant growth.

Description

Technical field [0001] The present invention belongs to the bioremediation of organic pollutants, and in particular relates to a citric acid and its Application in Portugal subtilis chloramphenicol and removal of the potassium phosphate solubilization solution. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is a first synthetic antibacterial antibiotics are a class of a class of broad-spectrum antibiotics, Gram-positive, negative bacteria are inhibited, especially typhoid, paratyphoid bacillus stronger effect its mechanism of action is to inhibit bacterial protein synthesis. Due to good antibacterial activity and pharmacokinetic properties, and is widely used in the treatment of human and animal diseases, especially for the control and treatment of various infectious diseases of all kinds of poultry, livestock, aquatic products play an important role. [0003] In recent years, chloramphenicol lead to irrational use of drug residues in soil, accumulation of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34A01N63/20A01P21/00C09K17/14C12R1/01C02F101/38C09K101/00
CPCC12N1/20B09C1/10C02F3/34A01N63/20C09K17/14C02F2101/38C09K2101/00
Inventor 李永涛谭泽文龚贝妮杨秀月刘怡灵陈恋
Owner SOUTH CHINA AGRI UNIV
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