A Strain of Aeromonas intermedia and Its Application in Removing Chloramphenicol and Solubilizing Phosphate and Potassium

A technology of Aeromonas and chloramphenicol, applied in the field of microbial remediation of organic pollutants, can solve the problems of difficult hydrolysis and photolysis, damage to bone marrow hematopoietic function, etc., and achieve the effect of increasing soil available phosphorus and potassium content

Active Publication Date: 2022-06-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CAP is stable and heat-resistant, so it is not easy to be hydrolyzed and photolyzed in the environment; and long-term and repeated use of CAP can damage the hematopoietic function of bone marrow and cause aplastic anemia

Method used

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  • A Strain of Aeromonas intermedia and Its Application in Removing Chloramphenicol and Solubilizing Phosphate and Potassium
  • A Strain of Aeromonas intermedia and Its Application in Removing Chloramphenicol and Solubilizing Phosphate and Potassium
  • A Strain of Aeromonas intermedia and Its Application in Removing Chloramphenicol and Solubilizing Phosphate and Potassium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Screening, isolation, purification and identification of strain SZW3

[0057] 1. Screening method of chloramphenicol degrading strain SZW3

[0058] 1. Material preparation

[0059] Strain screening sample source: An earthworm was excavated from a dry canal next to a pig farm in Longping Town, Lianzhou City, Qingyuan City, Guangdong Province. The earthworm and surrounding soil samples were sealed in a sampling bag, and brought back to the laboratory at 4°C. content.

[0060] LB medium: 10.0 g of peptone, 5.0 g of yeast extract powder, 10.0 g of NaCl, pH 7.0-7.2, dilute to 1 L with distilled water, and add 18.0 g of agar powder to the solid medium. Sterilize at 121°C for 15min.

[0061] Mineral salt medium (MSM): 5 mL of phosphate buffered solution (KH 2 PO 4 8.5g·L -1 , K 2 HPO 4 ·H 2 O21.75g·L -1 , Na 2 HPO 4 ·12H 2 O 33.4g·L -1 , NH 4 Cl 5.0g·L -1 ), 3.0mL 22.5g·L -1 MgSO 4 solution (MgSO 4 ·7H 2 O 46.125g·L -1 ), 1.0mL 0.25g·L -1 FeCl...

Embodiment 2

[0083] Example 2 Bacterial strain SZW3 removes chloramphenicol test

[0084] After streaking the strain SZW3 and culturing it on LB plate at 30°C for 20h, pick a single colony and inoculate it in LB liquid medium, cultivate it in a shaker at 30°C and 150rpm for 20h, and then inoculate it at a ratio of 2% (v / v). Chloramphenicol concentration is 10mg·L -1 contains 10g·L -1 In the inorganic salt liquid medium of peptone, 30℃, 150r·min -1 Shake culture for 0, 0.25, 0.5, 1, 2, 3, 4 and 6 days (d), with no addition of strain SZW3 as blank control (CK), three replicate experiments were set. The removal efficiencies of chloramphenicol were 0, 7.88, 20.76, 38.06, 55.86, 59.56, 64.01 and 64.28%, respectively.

[0085] Strain SZW3 contains 10g·L -1 The degradation kinetics of chloramphenicol in peptone MSM medium is shown in Fig. image 3 As shown, the degradation rate of chloramphenicol as well as strain growth and pH changes as Figure 4 As shown: the growth of SZW3 showed an "S-...

Embodiment 3

[0086] Qualitative test of dissolving phosphorus and potassium of embodiment 3 strain SZW3

[0087] Strain phosphate / potassium solubilizing bacteria (phosphate / potassium solubilizing bacteria, meaning converting insoluble phosphorus and potassium elements into soluble phosphorus and potassium) (specific references: Rawat, P., Das, S., Shankhdhar, D., et al., Phosphate-solubilizing microorganisms: Mechanism and their role in phosphate solubilization and uptake[J]. Journal of Soil Science and Plant Nutrition. 2020, https: / / doi.org / 10.1007 / s42729- 020-00342-7.) Functional test:

[0088] Strain SZW3 was inoculated in LB liquid medium at 125-150r·min -1, 28 ~ 30 ℃ shaking incubator for 18 ~ 24h, then 2% (v / v) was connected to NBRIP or potassium-solubilizing bacterial liquid medium, set up 3 repeated experiments, placed at 30 ℃, 150r·min -1 Incubate in a shaker for 6 days, and measure the content of available phosphorus and potassium in the solution. in,

[0089] NBRIP medium fo...

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Abstract

The invention discloses a strain of Aeromonas intermedia and its application in removing chloramphenicol and dissolving phosphorus and potassium. The name of the Aeromonas intermedia is Aeromonas intermedia (Aeromonas media) SZW3, and the preservation number is GDMCC NO: 61503. The strain was preserved in Building 59, Compound No. 100, Xianlie Middle Road, Guangzhou City on February 5, 2021 Guangdong Microbial Culture Collection Center on the 5th floor. Aeromonas intermedia SZW3 in the present invention can remove chloramphenicol through co-metabolism, and can also secrete organic acid substances to reduce the pH in the system, thereby dissolving insoluble phosphorus and potassium substances. Therefore, the bacterial strain and its culture can be applied to remove chloramphenicol in soil and water, and can be used to increase the content of available phosphorus and potassium in soil to promote plant growth.

Description

technical field [0001] The invention belongs to the technical field of microbial restoration of organic pollutants, and particularly relates to a strain of Aeromonas intermedia and its application in removing chloramphenicol and dissolving phosphorus and potassium. Background technique [0002] Chloramphenicol (CAP) was first discovered as an antibiotic produced by Actinomycete Streptomyces venezuela, and now it has been produced by chemical synthesis. As a broad-spectrum antibiotic, CAP has inhibitory effects on both gram-negative bacteria and positive bacteria, but the inhibitory effect on gram-negative bacteria is stronger than that of positive bacteria; its inhibitory mechanism is mainly to specifically prevent mRNA and ribosomes. bind, thereby preventing bacterial protein synthesis. CAP is mainly used for the treatment of typhoid, paratyphoid and typhus, as well as pertussis, trachoma, bacillary dysentery and urinary tract infection. [0003] Due to the low production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14B09C1/10C02F3/34C12R1/01C02F101/38
CPCC12N1/20C09K17/14B09C1/10C02F3/34C02F2101/38
Inventor 龚贝妮谭泽文李永涛徐会娟杨秀月徐雨晴
Owner SOUTH CHINA AGRI UNIV
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