Klebsiella variicola and application thereof in removal of chloramphenicol and cadmium ions

A technology of Klebsiella and Klebsiella, applied in the field of microbial remediation of organic pollutants, can solve problems such as secondary pollution, high energy consumption, and threats to ecosystems at trace levels, and achieve the goal of reducing chloramphenicol content Effect

Active Publication Date: 2021-11-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In traditional wastewater treatment, the main removal techniques of CAP include chemical precipitation, activated sludge degradation, adsorption, extraction, electrochemical, oxidation, reduction and other physical and chemical methods and biological methods. It has been reduced, but its trace level may also pose a threat to the ecosystem. At present, these methods for treating chloramphenicol wastewater have high degradation efficiency, but have the disadvantages of high energy consumption and secondary pollution.

Method used

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  • Klebsiella variicola and application thereof in removal of chloramphenicol and cadmium ions
  • Klebsiella variicola and application thereof in removal of chloramphenicol and cadmium ions
  • Klebsiella variicola and application thereof in removal of chloramphenicol and cadmium ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Screening, isolation and purification and identification of embodiment 1 bacterial strain YB1

[0065] 1. Screening method for chloramphenicol-degrading strain YB1

[0066] 1. Material preparation

[0067] Source of strain screening sample: An earthworm was excavated from a dry ditch next to a pig farm in Longping Town, Lianzhou City, Qingyuan, Guangdong Province. The earthworm and surrounding soil samples were sealed with sampling bags, brought back to the laboratory at 4°C, and immediately dissected to obtain intestinal tract contents.

[0068] LB medium: peptone 10.0g, yeast extract powder 5.0g, NaCl 10.0g, pH 7.0-7.2, distilled water to 1L, solid medium plus 18.0g agar powder. Sterilize at 121°C for 15 minutes.

[0069] Mineral salt medium (MSM): 5mL phosphate buffer solution (KH 2 PO 4 8.5g·L -1 、K 2 HPO 4 ·H 2 O2 1.75g·L -1 、Na 2 HPO 4 12H 2 O 33.4g·L -1 , NH 4 Cl 5.0g L -1 ), 3.0mL 22.5g·L -1 MgSO 4 Solution (MgSO 4 ·7H 2 O 46.125g·L -1 ), 1...

Embodiment 2

[0091] Example 2 Bacterial strain YB1 removes chloramphenicol test

[0092] Streak the strain YB1 on the LB plate and culture it at 30°C for 20h, pick a single colony and inoculate it in the LB liquid medium, culture it in the shaking table at 30°C and 150rpm for 20h, then inoculate it in the ratio of 2% (v / v) The concentration of chloramphenicol is 10mg·L -1 Contains 10g·L -1 In peptone MSM medium, 30°C, 150r min -1 Shaking culture was performed for 0, 0.25, 0.5, 1, 2, 3, 4, 5 and 6 days (d), with no addition of strain YB1 as the blank control (CK), and three repeated experiments were set up. Determination of strain YB1 to the removal amount and removal efficiency of chloramphenicol; Wherein the content of CAP is determined by HPLC (method is the same as in Example 1).

[0093] Strain YB1 contained 10g·L -1 Degradation kinetics of chloramphenicol in peptone MSM medium image 3 As shown, the degradation rate of chloramphenicol and the strain growth and pH changes as Fig...

Embodiment 3

[0094]The combined pollution test of cadmium ion and chloramphenicol of embodiment 3 bacterial strain YB1

[0095] Inoculate strain YB1 in LB liquid medium, at 125~150r·min -1 , 28~30℃ shaker shake culture for 18~24h, then inoculate Cd with 2% (v / v) inoculum 2+ (adding cadmium chloride) concentrations were 0, 2, 5, 10, 20, 50 mg / L and chloramphenicol concentration was 10 mg L -1 Contains 10g·L -1 In the MSM medium of peptone, set up 3 repeated experiments, place at 30°C, 150r min -1 Cultured in a shaker for 2 days. The removal amount and removal efficiency of cadmium ions and chloramphenicol were measured by bacterial strain YB1; wherein the content of CAP was measured by HPLC (the same method as in Example 1), and the content of cadmium ions was measured by AAS.

[0096] Strain YB1 contained cadmium ion concentrations of 0, 2, 5, 10, 20, 50 mg / L and chloramphenicol concentration of 10 mg·L -1 10g·L -1 The removal efficiency of cadmium ions in peptone MSM medium, as well...

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Abstract

The invention discloses Klebsiella variicola and an application thereof in removal of chloramphenicol and cadmium ions. The name of the strain is Klebsiella variicola YB1, the preservation number of the strain is GDMCCNO: 61696, and the strain is preserved in the Guangdong Microbial Culture Collection Center on the fifth floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou on May 27, 2021. The klebsiella variicola YB1 can remove chloramphenicol in a co-metabolism manner, and can also increase the pH value of a system to precipitate cadmium ions, so that the klebsiella variicola YB1 can be used for removing or reducing residues of chloramphenicol and cadmium ions in the environment.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic pollutants, in particular to a strain of Klebsiella mutans and its application in removing chloramphenicol and cadmium ions. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is a broad-spectrum antibiotic, anti-infective drugs. It inhibits the growth of bacteria by inhibiting the synthesis of bacterial proteins, and is used to treat infections caused by typhoid bacillus, Shigella, Escherichia coli, influenza bacillus, Brucella, pneumococcus, etc. It is widely used in clinical treatment and aquaculture of poultry and livestock, and can also be used as an animal growth promoter. It is usually white or colorless needle-like or flaky crystals, easily soluble in methanol, ethanol, propanol, chloramphenicol is extremely stable, and its aqueous solution does not lose efficacy even after being boiled for 5 hours. [0003] As chloramphenicol is widely used in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/22C02F101/38C02F101/20
CPCC12N1/20C02F3/34B09C1/10C02F2101/38C02F2101/20
Inventor 谭泽文杨秀月龚贝妮李永涛刘怡灵陈恋
Owner SOUTH CHINA AGRI UNIV
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