A strain of Pseudomonas taiwanensis capable of degrading tetracycline and its application

A Taiwan Pseudomonas and tetracycline technology, applied in the field of microbial remediation of organic pollutants, can solve problems such as environmental microbial diversity and ecological security threats

Active Publication Date: 2021-04-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that tetracycline residues have been detected in a variety of media, such as crops, soil, animal manure, irrigation water, and tap water, and even tetracycline pollution has been detected in groundwater; and tetracyclines enter the environment. Potential threats to soil fertility, plant growth, environmental microbial diversity, and ecological security

Method used

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  • A strain of Pseudomonas taiwanensis capable of degrading tetracycline and its application
  • A strain of Pseudomonas taiwanensis capable of degrading tetracycline and its application
  • A strain of Pseudomonas taiwanensis capable of degrading tetracycline and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening, separation, purification and identification of strain TC952

[0026] 1. Screening method of tetracycline-degrading strain TC952

[0027] 1. Material preparation

[0028] Sludge source for strain screening: Taken from the farmhouse fertilized vegetable field in Zuolong Village, Chaling County, Zhuzhou City, Hunan Province.

[0029] LB medium: 10.0 g of peptone, 5.0 g of yeast extract powder, 10.0 g of NaCl, pH 7.0-7.2, dilute to 1 L with distilled water, and add 18.0 g of agar powder to the solid medium. Sterilize at 121°C for 15min.

[0030] Mineral salt medium (MSM): 5 mL of phosphate buffered solution (KH 2 PO 4 8.5g·L -1 , K 2 HPO 4 ·H 2 O21.75g·L -1 , Na 2 HPO 4 ·12H 2 O 33.4g·L -1 , NH 4 Cl 5.0g·L -1 ), 3.0mL22.5g·L -1 MgSO 4 solution (MgSO 4 ·7H 2 O 46.125g·L -1 ), 1.0mL 0.25g·L -1 FeCl 3 solution (FeCl 3 ·6H 2 O 0.42g·L -1 ), 1.0mL 36.4g·L -1 CaCl 2 Solution (CaCl 2 ·2H 2 O 48.22 g·L -1 ), 1.0mL trace element so...

Embodiment 2

[0054] The determination of phosphorus-dissolving ability of embodiment 2 strain TC952

[0055]Qualitative test of the phosphorus-dissolving function of the strain: inoculate the strain TC952 into LB medium by streaking method, culture in a 30°C incubator for 24 hours, then pick a single colony and inoculate it on the PKO solid medium, and set up 3 repeated experiments , placed in a 37°C incubator for 5-7 days, observe whether there is a transparent circle formed by the hydrolysis of dissolved phosphorus around the colony in the medium, and observe the size of the transparent circle, measure the diameter of the hydrolysis circle and the colony circle with a ruler, and take pictures . According to the ratio of the diameter of the hydrolysis circle to the colony circle, it is judged whether the strain has phosphorus-dissolving effect and the relative size of the phosphorus-dissolving ability. Among them, PKO medium formula: glucose 10g L -1 , (NH 4 ) 2 SO 4 0.5g·L -1 , NaC...

Embodiment 3

[0057] Example 3 Degradation of Tetracycline by Bacterial Strain TC952 in Inorganic Salt Medium Containing Tetracycline and Peptone

[0058] Streak the strain TC952 on the LB plate and culture it at 30°C for 20 hours, pick a single colony and inoculate it in the LB liquid medium, 30°C, 150r min -1 After culturing in a shaker for 20 hours, inoculate at a ratio of 2% (v / v) to TC content of 5, 10, 20, 50 mg·L -1 containing 10g·L -1 Cultured in tryptone-based MSM medium, and samples were taken after 6 d (days); the strain TC952 was not added as a control (CK). After the solution was filtered, the residual amount of TC was measured by HPLC (tetracycline determination method was the same as that in Example 1). The results showed that after 6 days, the control tetracycline without adding bacteria was hydrolyzed, and after adding bacteria TC952, the degradation of tetracycline was significantly accelerated and strengthened. The hydrolysis rate was 32.70%, 33.18%, 23.26%, and 20.32%...

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Abstract

The invention discloses a strain of Pseudomonas taiwanensis capable of degrading tetracycline and its application. The name of the Pseudomonas taiwanensis is Pseudomonas taiwanensis TC952, and the preservation number is GDMCC NO:60636. species collection center. The Pseudomonas taiwanensis of the present invention can degrade tetracycline through co-metabolism, so it can be applied to the degradation of low-concentration tetracycline in soil and water, and can remove or reduce tetracycline in the environment for the degradation of medium and low-concentration tetracycline At the same time, the strain also has phosphorus-dissolving properties, and when applied to soil, it also has the effect of increasing soil phosphorus nutrients and enhancing soil fertility.

Description

technical field [0001] The invention belongs to the technical field of microbial restoration of organic pollutants, and particularly relates to a strain of Pseudomonas Taiwan which can degrade tetracycline and its application. Background technique [0002] Tetracycline (TC) is a broad-spectrum antibiotic used to treat infections caused by a variety of Gram-positive and negative bacteria, and has an effect on certain rickettsiae, viral viruses and protozoa. Due to its good bacteriostatic effect, it has been continuously used in the prevention and treatment of human and animal and plant diseases and the promotion of animal growth. After tetracycline is applied to the body, only a small part of it is absorbed, and it is not completely metabolized in the tissues; while most of it cannot be absorbed by the body (35-90%), and is excreted in urine and feces in the form of the original parent or metabolite Outside the body, and ultimately directly or indirectly into the environment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38C02F101/34C02F101/38
CPCB09C1/10C02F3/34C02F2101/34C02F2101/345C02F2101/38C12N1/20C12N1/205C12R2001/38
Inventor 龚贝妮谭泽文李永涛徐会娟杨行健
Owner SOUTH CHINA AGRI UNIV
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